Erianin Inhibits Proliferation and Induces Apoptosis of HaCaT Cells via ROS-Mediated JNK/c-Jun and AKT/mTOR Signaling Pathways

Mo, Canlong; Shetti, Dattatrya

doi:10.3390/molecules24152727

Cited by 35 publications

(26 citation statements)

References 43 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar substances were shown to have effects on keratinocytes or endothelial cells, which were also involved in diabetic wound healing. Mo et al explored the effect of erianin, a bibenzyl compound extracted from Dendrobium chrysotoxum, on proliferation and apoptosis in HaCaT cells and demonstrated that erianin could be recognized as a potential antipsoriasis drug that inhibited proliferation and induced apoptosis of HaCaT cells through ROS-mediated JNK/c-Jun and AKT/ mTOR signaling pathways [29]. Erianin was also found to induce a JNK/SAPK-dependent metabolic inhibition in human umbilical vein endothelial cells [30].…”

Section: Discussionmentioning

confidence: 99%

Effects of Dendrobium Polysaccharides on the Functions of Human Skin Fibroblasts and Expression of Matrix Metalloproteinase-2 under High-Glucose Conditions

Cao

et al. 2021

International Journal of Endocrinology

View full text Add to dashboard Cite

The effects of Dendrobium polysaccharides (PDC) on the functions of human skin fibroblasts (HSFs) and expression of matrix metalloproteinase-2 under high-glucose conditions and exploration of the underlying mechanism remain unclear. We used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis and flow cytometry to evaluate the cell viability and apoptosis. The collagen levels were determined by the Sircol™ Collagen Assay. Real-time quantitative polymerase chain reaction (RT-PCR) was used to detect the expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase inhibitor (TIMP-2) mRNA. We found the following: (1) under the high-glucose condition, the HSF cell viability, the expression of TIMP-2 mRNA, and the collagen levels were reduced, while the apoptosis rate and the expression of MMP-2 mRNA increased ( P < 0.05 ). (2) In the high-glucose + PDC group, the PDC reversed the changes in the collagen level, viability, and apoptosis rate of the HSF cells caused by high glucose, with the expression of protein and TIMP-2 mRNA increased and the level of MMP-2 mRNA decreased ( P < 0.05 ). This is the first time attempting to reveal that PDC can exhibit protective effects on HSF under high-glucose conditions, which may be related to the upregulation of the TIMP-2 expression and inhibition of the MMP-2 expression.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of Dendrobium Polysaccharides on the Functions of Human Skin Fibroblasts and Expression of Matrix Metalloproteinase-2 under High-Glucose Conditions

Cao

et al. 2021

International Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…Erianin, a low molecular weight natural product extracted from Dendrobium chrysotoxum Lindl, has been shown therapeutic potential to suppress tumor growth and angiogenesis in vivo and in vitro [8,9]. Recently, we have demonstrated that erianin exhibited growth suppression and apoptosis in HaCaT cells [10]. However, whether erianin induced HaCaT cell apoptosis via mitochondrial and ERS signaling pathways remains unclear.…”

mentioning

confidence: 99%

Development of erianin-loaded dendritic mesoporous silica nanospheres with pro-apoptotic effects and enhanced topical delivery

Mo¹,

Liu²,

Liu³

2020

J Nanobiotechnol

View full text Add to dashboard Cite

Background: Psoriasis is a malignant skin disease characterized as keratinocyte hyperproliferation and aberrant differentiation. Our previous work reported that a bibenzyl compound, erianin, has a potent inhibitory effect on keratinocyte proliferation. To improve its poor water-solubility, increase anti-proliferation activity, and enhance the skin delivery, erianin loaded dendritic mesoporous silica nanospheres (E/DMSNs) were employed. Results: In this work, DMSNs with pore size of 3.5 nm (DMSN 1) and 4.6 nm (DMSN 2) were fabricated and E/DMSNs showed pore-size-dependent, significantly stronger anti-proliferative and pro-apoptotic effect than free erianin on human immortalized keratinocyte (HaCaT) cells, resulting from higher cellular uptake efficiency. In addition, compared to free erianin, treatment with E/DMSNs was more effective in reducing mitochondrial membrane potential and increasing cytoplasmic calcium levels, which were accompanied by regulation of mitochondria and endoplasmic reticulum stress (ERS) pathway. Porcine skin was utilized in the ex vivo accumulation and permeation studies, and the results indicated higher drug retention and less drug penetration in the skin when administered as the E/DMSNsloaded hydrogel compared to the erianin-loaded hydrogel. Conlusions This work not only illustrated the further mechanisms of erianin in anti-proliferation of HaCaT cells but also offer a strategy to enhance the efficiency of erianin and the capacity of skin delivery through the DMSNs drug delivery systems.

show abstract

“…Injury subsequently reduces the antioxidant status as a consequence of an upregulation of ROS generation. ROSs are critical regulators of each step of tissue repair: while low concentrations of ROS generation are cell survival signaling, excessive production of ROS causes oxidative damage and impairs tissue repair, which is one of the main causes of refractory chronic wounds [ 29 , 46 – 52 ]. Malondialdehyde is a lipid peroxidation-derived product whose expression level is frequently used as a readout to evaluate the level of oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

“…This characterization was undertaken because our preliminary study showed that TNX was upregulated in both the stroma and epithelium in an injured mouse cornea during healing. The results clearly showed in a TNX-deficient (KO) mouse that there was impairment of epithelial healing in association with increased infiltration of neutrophils and upregulation of reactive oxygen species (ROS) in the tissues evaluated by the accumulation of ROS-derived product (malondialdehyde) [ 29 ]. This disrupted response included a delay in wound healing, which is attributable to loss of TNX’s control of neutrophil infiltration and regulation of local ROS because systemic ablation of neutrophils or chemical scavenging ROS rescued the KO phenotype.…”

Section: Introductionmentioning

confidence: 99%

Impairment of corneal epithelial wound healing is association with increased neutrophil infiltration and reactive oxygen species activation in tenascin X-deficient mice

Sumioka

Iwanishi

Okada

et al. 2021

Laboratory Investigation

View full text Add to dashboard Cite

The purpose of the study was to uncover the role of tenascin X in modulation of healing in mouse corneas subjected to epithelium debridement. Healing in corneas with an epithelial defect was evaluated at the levels of gene and protein expression. Wound healing-related mediators and inflammatory cell infiltration were detected by histology, immunohistochemistry and real-time RT-PCR. Tenascin X protein was upregulated in the wounded wild-type (WT) corneal epithelium. The lack of tenascin X impaired closure of an epithelial defect and accelerated infiltration of neutrophils into the wound periphery as compared to the response in WT tissue. Expression of wound healing-related proinflammatory and reparative components, i.e., interleukin-6, transforming growth factor β, matrix metalloproteinases, were unaffected by the loss of tenascin X expression. Marked accumulation of malondialdehyde (a lipid peroxidation-derived product) was observed in KO healing epithelia as compared with its WT counterpart. Neutropenia induced by systemic administration of a specific antibody rescued the impairment of epithelial healing in KO corneas, with reduction of malondialdehyde levels in the epithelial cells. Finally, we showed that a chemical scavenging reactive oxygen species reversed the impairment of attenuation of epithelial repair with a reduction of tissue levels of malondialdehyde. In conclusion, loss of tenascin X prolonged corneal epithelial wound healing and increased neutrophilic inflammatory response to debridement in mice. Tenascin X contributes to the control of neutrophil infiltration needed to support the regenerative response to injury and prevent the oxidative stress mediators from rising to cytotoxic levels.

show abstract

Erianin Inhibits Proliferation and Induces Apoptosis of HaCaT Cells via ROS-Mediated JNK/c-Jun and AKT/mTOR Signaling Pathways

Cited by 35 publications

References 43 publications

Effects of Dendrobium Polysaccharides on the Functions of Human Skin Fibroblasts and Expression of Matrix Metalloproteinase-2 under High-Glucose Conditions

Effects of Dendrobium Polysaccharides on the Functions of Human Skin Fibroblasts and Expression of Matrix Metalloproteinase-2 under High-Glucose Conditions

Development of erianin-loaded dendritic mesoporous silica nanospheres with pro-apoptotic effects and enhanced topical delivery

Impairment of corneal epithelial wound healing is association with increased neutrophil infiltration and reactive oxygen species activation in tenascin X-deficient mice

Contact Info

Product

Resources

About