Erhualian and Pietrain pigs exhibit distinct behavioral, endocrine and biochemical responses during transport

Li, Liuan; Xia, Dong; Bao, Endong; Shi, Wei; Xiao, Jin-Song; Bao, Jing-Wei; Chen, Weihua; Chen, Jie; Hartung, J.; Zhao, Ruqian

doi:10.1016/j.livsci.2007.03.008

Cited by 35 publications

(34 citation statements)

References 43 publications

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“…In the two social stressors investigated, we found breed-specific coping responses to social isolation stress on the farm and transport stress with LY pigs showing higher cortisol concentration in comparison with LYLD pigs. Although this corroborates previously reported results of breed differences in pigs (Li et al, 2008), it may be that our LY pigs generally responded more toward different stressors due to having a higher diurnal activity. The difference in the basal stress axis activity was found between sexes as well, with castrated males having higher activity than females.…”

Section: Discussionsupporting

confidence: 93%

Peripheral regulation of stress and fear responses in pigs from tail-biting pens

Zupan

Zanella

2017

R. Bras. Zootec.

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-This study focused on stress physiology by characterizing cortisol responses to stressors in tail biters (n = 10), victims (n = 10), and control pigs (n = 10) of two different breeds (Landrace × Yorkshire, LY; Landrace/Yorkshire × Landrace/ Duroc; LYLD) and sexes (females and castrated males). We exposed pigs to 10 min of isolation with a human at the farm and transported them to a controlled environment. There, the behaviour was registered for 10 min during sessions when subjected to a novel object test and to a novel arena test. Sampling times of salivary cortisol were in all the fear tests before testing and 60 min thereafter, while for transportation as well 120 min after the beginning of transportation. We additionally measured cortisol at 7:00 and 16:00 h during three days following transportation. The basal stress axis activity followed a distinct diurnal rhythm between sexes and breeds, with castrated males having higher cortisol level than females and LY pigs higher than LYLD pigs. Following isolation at the farm and transportation, the concentration of salivary cortisol was higher in LY than in LYLD pigs. Pigs considered the exposure to a novel arena, but not to a novel object, stressful by showing a cortisol level after testing higher than before testing. The results suggest a genotypic effect on sensitivity to stress in pigs that have performed tail biting, have been victimized, or have not been involved in tail biting.

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Section: Discussionsupporting

confidence: 93%

Peripheral regulation of stress and fear responses in pigs from tail-biting pens

Zupan

Zanella

2017

R. Bras. Zootec.

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“…In previously studies it has been confirmed that there is different stress response between the two breeds (Désautés et al 1999, Li et al 2008a). The present study implied the different capacity of gluconeogenesis may be partly responsible for the distinct stress reaction.…”

Section: Discussionmentioning

confidence: 72%

Breed-specific patterns of hepatic gluconeogenesis and glucocorticoid action in pigs

Yang

Albrecht

et al. 2012

Arch. Anim. Breed.

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In the present study, Erhualian and Pietrain pigs were employed to investigate the breedspecific patterns of hepatic gluconeogenesis by detecting the related enzyme mRNA expression, and to analyse the relationship with the hepatic glucocorticoid receptors and 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) expression. Furthermore, hepatic DNA methyltransferase 1 (DNMT1) expression was determined to detect DNA methyltransferase state difference between the two breeds. The results demonstrated that the Erhualian pigs exhibited significantly lower plasma lactate acid concentration, but higher liver lactate acid content than the Pietrain pigs. A significantly higher expression of pyruvate carboxylase (PC), fructose-1, 6-bisphosphatase (FBP) and the mitochondria phosphoenolpyruvate carboxykinase (PCK2) mRNA were observed in Erhualian pigs. The Erhualian pigs demonstrated a significantly higher expression of glucocorticoid receptor and 11β-HSD1 mRNA than the Pietrain pigs, though there was no difference in the hepatic cortisol content between the two breeds. The hepatic DNMT1 mRNA expression in the Erhualian pigs was significantly lower, and the DNMT1 protein content in Erhualian pigs tended to be decreased compared with Pietrain pigs (P=0.066). The results suggest that the Erhualian pigs demonstrated higher hepatic gluconeogenesis capacity in comparison to the Pietrain pigs. The up-regulation of hepatic glucocorticoid receptor and 11β-HSD1 expression may be involved in the enhanced hepatic gluconeogenesis in Erhualian pigs. Moreover, the down-regulation of DNMT1 in Erhualian pigs implies possible involvement of DNA methylation in a breed-specific pattern of hepatic gluconeogenesis.

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“…This stress may induce intense physiological changes resulting in reduction in the animal's productivity and the quality of its products (von Borell and Schäffer 2005). Enzyme activities of CK and AST in the blood serum are often interpreted as an index of muscle damage and muscle fatigue (Fàbrega et al 2002;Pérez et al 2002;Li et al 2007). CK mainly distributes in cardiac muscle fiber; when it gets pathological damage, metabolic enzyme in cardiac muscle fiber will release into the blood.…”

Section: Discussionmentioning

confidence: 99%

Expression of heat shock protein 60 in the tissues of transported piglets

Zhu

Bao

Zhao

et al. 2009

Cell Stress and Chaperones

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In this study, we sought to determine the distribution and expression of heat shock protein 60 (Hsp60) in the tissues of transported piglets. A total of 24 Chinese Erhualian piglets with an average body weight of 20±1 kg were assessed under both 2-h transported and normal housing conditions. Results of enzymatic analysis showed that the serum creatine kinase and aspartate aminotransferase concentrations were significantly increased in the 2-h transported piglets. Acute cellular lesions characterized by granular and vacuolar degeneration of the parenchyma cells in the tested heart, liver, and kidney were also confirmed by histopathological test after 2 h transportation. These results indicate that transport stress induces tissue damage to heart, liver, and kidney. Hsp60-positive immunostaining was consistently detected in the cytoplasm of myocardial cells, hepatocytes, renal tubular epithelial cells, and epithelial cells of fundic gland. However, results of enzyme-linked immunosorbent assay indicated that Hsp60 expression was only significantly elevated in the stomach, with lower expression in the heart and a nonsignificant trend of increased liver and kidney expression of Hsp60. These results indicate that different tissues had different sensitivities to transport stress, possibly resulting in varying levels of cytoprotection by Hsp60 in the different tissues. The expression of Hsp60 following 2 h transportation coincided with deterioration of cardiac cytoprotection in the heart and protection in the stomach. However, the direct role of Hsp60 in cytoprotection of heart and stomach tissues needs further investigation.

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Erhualian and Pietrain pigs exhibit distinct behavioral, endocrine and biochemical responses during transport

Cited by 35 publications

References 43 publications

Peripheral regulation of stress and fear responses in pigs from tail-biting pens

Peripheral regulation of stress and fear responses in pigs from tail-biting pens

Breed-specific patterns of hepatic gluconeogenesis and glucocorticoid action in pigs

Expression of heat shock protein 60 in the tissues of transported piglets

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