Ergosterol content as a measure of biomass of potential biological control fungi in liquid cultures

Dawson-Andoh, Benjamin

doi:10.1007/s00107-001-0274-9

Cited by 10 publications

(5 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is suggested that perhaps the abundance of hypha present may have led to higher ergosterol levels, as ergosterol is a primary sterol found in the cell membrane of fungi [41], [42], [43]. The cell membrane acts as a barrier between an organelle and its environment, and also serving as a matrix for the association of proteins with lipids [44].…”

Section: Discussionmentioning

confidence: 99%

Understanding colonization and proliferation potential of endophytes and pathogen in planta via plating, polymerase chain reaction, and ergosterol assay

Chow

Rahman

Ting

2017

Journal of Advanced Research

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Understanding colonization and proliferation potential of endophytes and pathogen in planta via plating, polymerase chain reaction, and ergosterol assay

Chow

Rahman

Ting

2017

Journal of Advanced Research

View full text Add to dashboard Cite

show abstract

“…The pentane fraction was concentrated in a rotary evaporator and redissolved in 3 mL of methanoldichloromethane mixture (1:1). The method of ergosterol extraction and detection used in this study was according to Dawson-Andoh (2002).…”

Section: Extraction Of Ergosterolmentioning

confidence: 99%

Determination of Ergosterol as a Potential Biomarker in Pathogenic Medically Important Fungal Isolates

Lafi¹,

Santhanam

Khaithir

et al. 2018

JSKM

View full text Add to dashboard Cite

Ergosterol, a component of fungal cell membrane, has been frequently detected as an indicator of fungal presence and mass in environmental samples like soil. However, its detection in major pathogenic fungal species has not been investigated. In this study, the ergosterol contents of ten pathogenic fungal species were determined. Liquid chromatography was used for the detection and quantification of ergosterol extracted from fungal broth cultures. Results showed that ergosterol eluted as a single, well resolved peak in the chromatogram profiles of all tested fungi. Based upon relative amounts of ergosterol produced per fungal mycelial dry weight, three groups of fungal pathogens were identified, namely low ergosterol (Aspergillus niger, Candida albicans and Cryptococcus neoformans at 4.62, 6.29 and 7.08 µg/mg, respectively), medium ergosterol (Fusarium solani, Aspergillus fumigatus, Mucor sp., Penicillium sp., Cryptococcus gattii and Rhizopus sp. at 9.40, 10.79, 10.82, 11.38, 12.60 and 13.40 µg/mg, respectively), and high ergosterol (Candida tropicalis at 22.84 µg/ mg), producers. Ergosterol was not detectable in bacterial samples, which were included as controls. This first report on ergosterol detection in major pathogenic fungal species indicates that ergosterol may be used as a biomarker to diagnose invasive fungal infections in clinical samples. ABSTRAK Ergosterol, yang merupakan komponen membran sel fungus, seringkali dikesan sebagai petunjuk kehadiran dan jumlah fungus dalam sampel daripada persekitaran. Walau bagaimanapun, pengesanan ergosterol dalam fungus patogen utama belum dikaji. Dalam kajian ini, kandungan ergosterol dalam sepuluh spesies fungus patogen telah ditentukan. Kaedah kromatografi cecair digunakan untuk mengesan kehadiran dan penentuan kuantiti ergosterol yang diekstrak daripada kultur kaldu fungus. Berdasarkan kuantiti relatif ergosterol yang terhasil pada miselia fungus (berat kering), tiga kumpulan dikenal pasti iaitu mengandungi, ergosterol rendah (Aspergillus niger, Candida albicans dan Cryptococcus neoformans pada 4.62, 6.29 dan 7.08 µg/mg masing-masing), ergosterol sederhana (Fusarium solani, Aspergillus fumigatus, Mucor sp., Penicillium sp., Cryptococcus gattii dan Rhizopus sp. pada 9.40, 10.79, 10.82, 11.38, dan ergosterol tinggi (Candida tropicalis pada 22.84 µg/mg). Ergosterol tidak dapat dikesan pada sampel bakteria yang digunakan sebagai kawalan. Laporan pertama pengesanan ergosterol pada fungus patogen utama ini, menunjukkan bahawa ergosterol mungkin boleh digunakan sebagai biomarker untuk pendiagnosan jangkitan fungus invasif dalam sampel klinikal. Kata kunci: HPLC; ergosterol; biomarker; patogenik; fungus JSKM16(2) 3.indd 15

show abstract

“…The investigations are aimed at finding the correlation between the ergosterol level and the degree of substrate's fungi infestation, as well as preparation of standards that would specify the permitted amount of this compound in materials. However, the ergosterol content in materials infested with fungi does not have to be correlated with the mycelium's biomass, because it depends on many factors such as: fungus species, time and conditions of growth, composition of nutritive substances in the medium, temperature and pH [11]. Additionally, research has revealed that the addition of fungicides and heavy metals in a concentration that stops metabolic activity of chosen white-rot wood-decay fungi does not influence the ergosterol content [12,13].…”

Section: Structural Health Assessment Of Timber Structuresmentioning

confidence: 99%

Influence of Fungi on the State of Preservation and on the Usage Prospects of Antique Wooden Floor from Manor Houses in South-Eastern Poland

Andres

Różańska

Jankowska

et al. 2013

AMR

View full text Add to dashboard Cite

Floor structures are an integral part and a valuable element of antique buildings, and the only limitation of their preservation consists in their usage properties. A thorough study of their state of preservation is the starting point for choosing the correct conservation programmes. Currently, fungi are the main reason that leads to the replacement of antique wooden parquets in Poland. Simple visual inspections in many cases do not ensure a precise evaluation of the level of fungal infestation and the depth of penetration of the myceliums hyphae in the wood. Tests of ergosterol, which is a component of most fungis cell membrane, allow us to monitor the level of fungi infestation of diverse materials. This makes it possible to determine the depth of mycelium penetration within the wood quite precisely. Unfortunately, in case of basidiomycota it is not possible to correlate ergosterol with the myceliums biomass nor with the grade of wood degradation. Moreover, tests of ergosterol are not able to answer the question whether the fungi infestation is active, because this substance is also present in dead fungi hyphae. The presence of ergosterol in the wood can only give us a hint of presence of a substrate mycelium, which is responsible for the secretion of exoenzymes that degrade structural substances. Therefore, only the test results of ergosterol content and of the woods chemical composition, wettability curve and moisture equivalent, as well as computer simulations of its capacity to transfer own and usage loads (on the basis of the elasticity modulus, bending strength and wood density) together with the hardness, resistance to abrasion and resistance to scratches of wooden parquets permit us to take a correct decision as to the possibility of their further usage. The research carried out shows that, in many cases, parquets infested with fungi can be used safely. The article presents the proprieties that are important in the correct assessment of antique wooden parquets state of preservation and usage prospects. It specifies the factors that permit to eliminate the problem of fungi infestation and describes the proper usage conditions, proposing efficient methods of conservation.

show abstract

Ergosterol content as a measure of biomass of potential biological control fungi in liquid cultures

Cited by 10 publications

References 16 publications

Understanding colonization and proliferation potential of endophytes and pathogen in planta via plating, polymerase chain reaction, and ergosterol assay

Understanding colonization and proliferation potential of endophytes and pathogen in planta via plating, polymerase chain reaction, and ergosterol assay

Determination of Ergosterol as a Potential Biomarker in Pathogenic Medically Important Fungal Isolates

Influence of Fungi on the State of Preservation and on the Usage Prospects of Antique Wooden Floor from Manor Houses in South-Eastern Poland

Contact Info

Product

Resources

About