Ergosterol biosynthesis pathway in Aspergillus fumigatus

Alcàzar-Fuoli, Laura; Mellado, Emilia; García‐Effrón, Guillermo; López, Jordi F.; Grimalt, Joan O.; Cuenca-Estrella, J. Manuel; Rodríguez-Tudela, Juan Luis

doi:10.1016/j.steroids.2007.11.005

Cited by 128 publications

(144 citation statements)

References 40 publications

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“…We know of no study that found PTMEs in rice seeds but arundoin (fern-9(11)-en-3β-ol ME) and cylindrin (arbor-9(11)-en-3β-ol ME) have been detected in rice leaves (Ohmoto et al, 1970b). We did not detect any miliacin in the rice on which C. olivaceum developed but ergosterol, which is known to be synthetized de novo by fungi (Fryberg et al, 1973;Alcazar-Fuoli et al, 2008) and is therefore classically used as a fungal biomarker (Grant and West, 1986), was detected in addition to the sterols found in the control rice.…”

Section: Miliacin In Chaetomium Olivaceummentioning

confidence: 55%

Distribution of miliacin (olean-18-en-3β-ol methyl ether) and related compounds in broomcorn millet (Panicum miliaceum) and other reputed sources: Implications for the use of sedimentary miliacin as a tracer of millet

Bossard

Jacob

Milbeau

et al. 2013

Organic Geochemistry

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bution of miliacin (olean-18-en-3β-ol methyl ether) and related compounds in broomcorn millet (Panicum miliaceum) and other reputed sources: Implications for the use of sedimentary miliacin as a tracer of millet. Organic Geochemistry, Elsevier, 2013Elsevier, , 63, pp.48-55. <10.1016Elsevier, /j.orggeochem.2013 Miliacin biosynthesis has been proposed for other Panicum species, Setaria italica (Italian or foxtail millet), Pennisetum sp., and Chaetomium olivaceum (an olive green mold). We found miliacin concentrations in seeds of different varieties of P. miliaceum to be similarly high (with trace amounts of β-and α-amyrin methyl ethers). It was absent from hulls and roots, and nominally present in leaves and stems. The transfer of miliacin from plant to sediments is therefore mostly from seeds. Miliacin was abundant (often with larger amounts of β-and α-amyrin methyl ethers) in all other Panicum species studied but only in some species of the genus Pennisetum and absent in * Corresponding author e-mail address: jeremy.jacob@univ-orleans.fr 2 Setaria italica. Neither C. olivaceum nor its growth medium (rice) showed any trace of miliacin.Our results of no miliacin from S. italica and C. olivaceum , high miliacin concentrations in seed of P. miliaceum relative to other PTMEs and to other grasses, and considering the high biomass that cultivated broomcorn millet has relative to other potential plant sources, support the use of sedimentary records of miliacin in some contexts, to track past millet agricultural dynamics.

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Section: Miliacin In Chaetomium Olivaceummentioning

confidence: 55%

Distribution of miliacin (olean-18-en-3β-ol methyl ether) and related compounds in broomcorn millet (Panicum miliaceum) and other reputed sources: Implications for the use of sedimentary miliacin as a tracer of millet

Bossard

Jacob

Milbeau

et al. 2013

Organic Geochemistry

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“…Sterols were extracted from C. tropicalis after 18 h of growth in Sabouraud liquid medium at 37°C with shaking (150 rpm), using the protocol described previously (44,45). Briefly, cells were harvested by centrifugation, washed with sterile water, lyophilized, and weighed to determine the dry weight (in grams) of the fungus.…”

Section: Methodsmentioning

confidence: 99%

Candida tropicalis Antifungal Cross-Resistance Is Related to Different Azole Target (Erg11p) Modifications

Forastiero

Mesa-Arango

Alastruey‐Izquierdo

et al. 2013

Antimicrob Agents Chemother

100

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“…Sterols were extracted following 24-h growth in liquid minimal medium under iron-depleted or iron-replete conditions as previously described (54). To 100-200 mg of freeze-dried mycelia, 3 mL of 25% alcoholic (3:2 methanol:ethanol) potassium hydroxide solution was added.…”

Section: Methodsmentioning

confidence: 99%

“…Injector, transfer line, and ion source were at 300, 200, and 250°C, respectively. Sterols were identified by comparison of mass spectra and retention time data (54). Relative sterol composition was expressed as the ratio of raw peak areas with respect to the total sterol content.…”

Section: Methodsmentioning

confidence: 99%

Mevalonate governs interdependency of ergosterol and siderophore biosyntheses in the fungal pathogen Aspergillus fumigatus

Yasmin

Alcàzar-Fuoli

Gründlinger

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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Aspergillus fumigatus is the most common airborne fungal pathogen for humans. In this mold, iron starvation induces production of the siderophore triacetylfusarinine C (TAFC). Here we demonstrate a link between TAFC and ergosterol biosynthetic pathways, which are both critical for virulence and treatment of fungal infections. Consistent with mevalonate being a limiting prerequisite for TAFC biosynthesis, we observed increased expression of 3-hydroxy-3-methyl-glutaryl (HMG)-CoA reductase (Hmg1) under iron starvation, reduced TAFC biosynthesis following lovastatin-mediated Hmg1 inhibition, and increased TAFC biosynthesis following Hmg1 overexpression. We identified enzymes, the acyl-CoA ligase SidI and the enoyl-CoA hydratase SidH, linking biosynthesis of mevalonate and TAFC, deficiency of which under iron starvation impaired TAFC biosynthesis, growth, oxidative stress resistance, and murine virulence. Moreover, inactivation of these enzymes alleviated TAFC-derived biosynthetic demand for mevalonate, as evidenced by increased resistance to lovastatin. Concordant with bilateral demand for mevalonate, iron starvation decreased the ergosterol content and composition, a phenotype that is mitigated in TAFC-lacking mutants.siderophore | isoprenoide | statins

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Ergosterol biosynthesis pathway in Aspergillus fumigatus

Cited by 128 publications

References 40 publications

Distribution of miliacin (olean-18-en-3β-ol methyl ether) and related compounds in broomcorn millet (Panicum miliaceum) and other reputed sources: Implications for the use of sedimentary miliacin as a tracer of millet

Distribution of miliacin (olean-18-en-3β-ol methyl ether) and related compounds in broomcorn millet (Panicum miliaceum) and other reputed sources: Implications for the use of sedimentary miliacin as a tracer of millet

Candida tropicalis Antifungal Cross-Resistance Is Related to Different Azole Target (Erg11p) Modifications

Mevalonate governs interdependency of ergosterol and siderophore biosyntheses in the fungal pathogen Aspergillus fumigatus

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