Eremothecium ashbyi causes soybean yeast-spot and is associated with stink bug, Riptortus  clavatus

Kimura, Shigemitsu; Tokumaru, Susumu; Kuge, Kazuhiko

doi:10.1007/s10327-008-0097-1

Cited by 15 publications

(14 citation statements)

References 8 publications

(20 reference statements)

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“…In Japan, E. coryli and E. ashbyi are known as causal agents of yeast spot of soybean (Kimura 2007;Kimura et al 2008). To our knowledge, no disease of azuki bean caused by E. coryli and E. ashbyi has ever been reported.…”

Section: Name Of the Diseasementioning

confidence: 83%

“…Results of an assimilation test with API 20C AUX were in accordance with the previous description of E. ashbyi (de Hoog et al 1998) (Table 1). These isolates closely matched E. ashbyi, which was isolated and identified by Kimura et al (2008), in cultural, morphological and biological characteristics.…”

Section: Identification Of the Pathogenmentioning

confidence: 83%

“…Shin-Kyotodainagon), which had been sucked by bean bugs, and were identified using methods previously described (Kimura 2007;Kimura et al 2008). Of 27 isolates 19 (AZC1-19) produced yeastlike colonies that were a light cream color, pasty and slightly raised on solid nutrient agar (SNA) (Clarke and Wilde 1970).…”

Section: Identification Of the Pathogenmentioning

confidence: 99%

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Eremothecium coryli and E. ashbyi cause yeast spot of azuki bean

Kimura¹,

Tokumaru²,

Kuge³

2009

J Gen Plant Pathol

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Yeast-like fungi were isolated from lesions on azuki bean (cv. Shin-Kyotodainagon) seeds that had been sucked by bean bugs in Kyoto Prefecture, Japan. On the basis of morphological and physiological characteristics and sequence data of the internal transcribed spacer (ITS) regions including the 5.8S rDNA, these yeasts were identified as Eremothecium coryli and E. ashbyi. Pathogenicity of those yeasts was confirmed by a reinoculation test. To our knowledge, this is the first report of the occurrence of yeast spot in azuki bean in Japan.

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Section: Name Of the Diseasementioning

confidence: 83%

Section: Identification Of the Pathogenmentioning

confidence: 83%

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Eremothecium coryli and E. ashbyi cause yeast spot of azuki bean

Kimura¹,

Tokumaru²,

Kuge³

2009

J Gen Plant Pathol

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“…Sections on CMA+CC plates were observed with an inverted microscope at 1 day intervals. Eremothecium species were identified from the elongating mycelia or multiplying buds on CMA+CC plates using methods previously described (Kimura 2007;Kimura et al 2008b). …”

Section: Microscopic Examination Of Field-collected Bugsmentioning

confidence: 99%

“…clavatus), Nezara antennata Scott, Piezodorus hybneri (Gmelin) and Dolycoris baccarum (Linnaeus), are associated with yeast spot in Japan (Kimura et al 2008a). In addition, E. ashbyi (Guilliermond ex Routien) Batra was shown to cause yeast spot and, E. coryli was transmitted by the true bug R. pedestris (Kimura et al 2008b). Clarke and Wilde (1970) showed Acrosternum hilare (Say) is capable of retaining E. coryli throughout their adult life span once they acquire it.…”

Section: Introductionmentioning

confidence: 98%

Mode of transmission and morphological structures of two Eremothecium species between Riptortus pedestris and soybean

Kimura¹,

Tokumaru²,

Kuge³

2008

J Gen Plant Pathol

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Eremothecium ashbyi and E. coryli were present in the maxillary and mandibular stylet pouches of the contaminated heads of the true bug Riptortus pedestris collected during a field survey, 2007. Ascospores of E. ashbyi and E. coryli were taken up in the stylet pouch when R. pedestris fed on soybean seeds infected with the respective fungus. In the case of E. ashbyi, the ascospores had subsequently germinated and were recognized as large masses of mycelia in the stylet pouch. In contrast, masses of E. coryli including buds of irregular size and shape were recognized about 3 days after. These results proved that these yeasts are taken up and become lodged in the stylet pouch when the insect feeds on infected host plants. In stylet sheaths stained with erythrosine, E. ashbyi was found as fragments of mycelia and E. coryli was found as small buds. These observations yield evidence to confirm that E. ashbyi and E. coryli are transmitted as fragments of mycelia and as small buds, respectively, after the insect has fed on infected host plants.

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Potential for Nezara viridula (Hemiptera: Pentatomidae) to Transmit Bacterial and Fungal Pathogens into Cotton Bolls

et al. 2009

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Recently, we showed that the southern green stink bug (SGSB), Nezara viridula (L.), can transmit Pantoea agglomerans (Ewing and Fife), an opportunistic bacterium, into green cotton bolls resulting in plant disease. Here, we hypothesized that our established model could be used to determine if the SGSB was a general, non-discriminate vector by using two other opportunistic bacterial pathogens of bolls (Pantoea ananatis [Serano] and Klebsiella pneumoniae [Schroeter]) and the known fungal pathogen Nematospora coryli (Peglion). Variants of P. ananatis (strain Pa-1R) and K. pneumoniae (strain Kp 5-1R) selected for rifampicin (Rif) resistance were used as bacterial opportunists. N. coryli was detected only from laboratory-reared SGSB directly exposed to the fungus. Both Pa-1R and Kp 5-1R were recovered from SGSB previously provided a contaminated food source (2 days), sterile food (5 days), and then harvested after being caged on bolls (2 days) at levels reaching 10(3) and 10(4) colony forming units (cfus) per insect, respectively. However, bolls caged with insects infected with Pa-1R or Kp 5-1R and with evidence of feeding did not become diseased nor were either opportunists detected from boll tissues. Insects infected with N. coryli transmitted the pathogen, which resulted in diseased bolls and fungi concentrations reached 10(6) cfus/g locule tissue at 2 weeks following the caging period. Notably, each of the three pathogens independently caused boll disease when mechanically inoculated using a needle puncture. Generally, these results suggest that cotton pathogen acquisition by the SGSB was not sufficient to determine whether the insects were disease vectors of the opportunists.

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Eremothecium ashbyi causes soybean yeast-spot and is associated with stink bug, Riptortus clavatus

Cited by 15 publications

References 8 publications

Eremothecium coryli and E. ashbyi cause yeast spot of azuki bean

Eremothecium coryli and E. ashbyi cause yeast spot of azuki bean

Mode of transmission and morphological structures of two Eremothecium species between Riptortus pedestris and soybean

Potential for Nezara viridula (Hemiptera: Pentatomidae) to Transmit Bacterial and Fungal Pathogens into Cotton Bolls

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