Eremostachys Binalodensis, A Potential Therapeutic Choice for Gingival Inflammatory Wounds

Abstract

Objectives: In this study we aimed to evaluate the effect of E. binalodensis on gingival inflammatory wounds. Methods: In-vitro wound was induced by scratching the surface layer of human gingival fibroblasts (hGFs). Cells were pretreated with 1,10,100,1000 µg/ml of E. binalodensis methanol extract prior to 1µg/ml LPS stimulation. hGFs proliferation was assessed by MTT test. Also levels of critical inflammatory cytokines such as IL-1𝛽, IL-6 and TNF-𝛼 were determined by enzyme-linked immunosorbent assay (ELISA). Results: Wound induction was associated with secretion of IL-1β, IL-6 and TNF-α from hGFs. E. binalodensis enhanced the hGFs proliferation besides reducing the level of IL-1β, IL-6 and TNF-α in LPS-scratch-stimulated hGFs. Conclusions: Regarding anti-inflammatory and proliferative effects of E. binalodensis on hGFs, availability and safety of it, it is suggested for enhancing the wound healing process in gingival inflammatory wounds.