ERECTA family signaling constrains<i>CLAVATA3</i>and<i>WUSCHEL</i>to the center of the shoot apical meristem

Zhang, Liang; DeGennaro, Daniel; Lin, Ge; Chai, Jijie; Shpak, Elena D.

doi:10.1101/2020.02.24.962787

“…In [42] it is discussed that a dose-dependent inhibitory effect of WUS on proliferation rates may play a role in this context. Furthermore, ERECTA signalling may be involved in confining the WUS and CLV3 expression to the meristem centre by repressing both signals [48,49]. These mechanisms could be compared in future extension of our model.…”

Section: Discussionmentioning

confidence: 98%

Mathematical modeling of plant cell fate transitions controlled by hormonal signals

Klawe

¹

,

Stiehl

²

,

Bastian

³

et al. 2019

Preprint

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Coordination of fate transition and cell division is crucial to maintain the plant architecture and to achieve efficient production of plant organs. In this paper, we analysed the stem cell dynamics at the shoot apical meristem (SAM) that is one of the plant stem cells locations. We designed a mathematical model to elucidate the impact of hormonal signaling on the fate transition rates between different zones corresponding to slowly dividing stem cells and fast dividing transit amplifying cells. The model is based on a simplified two-dimensional disc geometry of the SAM and accounts for a continuous displacement towards the periphery of cells produced in the central zone. Coupling growth and hormonal signaling results in a nonlinear system of reaction-diffusion equations on a growing domain with the growth velocity depending on the model components. The model is tested by simulating perturbations in the level of key transcription factors that maintain SAM homeostasis. The model provides new insights on how the transcription factor HECATE is integrated in the regulatory network that governs stem cell differentiation. 1 SummaryPlants continuously generate new organs such as leaves, roots and flowers. This process is driven by stem cells which are located in specialized regions, so-called meristems. Dividing stem cells give rise to offspring that, during a process referred to as cell fate transition, become more specialized and give rise to organs. Plant architecture and crop yield crucially depend on the regulation of meristem dynamics. To better understand this regulation, we develop a computational model of the shoot meristem. The model describes the meristem as a two-dimensional disk that can grow and shrink over time, depending on the concentrations of the signalling factors in its interior. This allows studying how the non-linear interaction of multiple transcription factors is linked to cell division and fate-transition. We test the model by simulating perturbations of meristem signals and comparing them to experimental data. The model allows simulating different hypotheses about signal effects. Based on the model we study the specific role of the transcription factor HECATE and provide new insights in its action on cell dynamics and in its interrelation with other known transcription factors in the meristem. 4 ing molecules. Such models have been applied e.g., to investigate cytokinin signaling [11] and patterning of the shoot apical meristem [14,17]. To investigate the impact of HEC on fate transition and proliferation rates of cells in the CZ and PZ, we have recently proposed a model based on the population dynamics approach, in which dynamics of different cell subpopulations are described by ordinary differential equations [9]. Such approach allows tracking how changes in cell proliferation, fate transition, primordia formation and primordia separation affect the time evolution and steady-state size of the different SAM zones but does not take into account spatio-temporal dynamics of the underlying signal...

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“…In contrast, in A. thaliana the close homologs EPFL4 and EPFL6 have been shown to have distinct functions in inflorescence growth 62 , 63 . However, two very recent studies revealed that EPFL4 and EPFL6 are also expressed in the periphery of the shoot apical meristem (SAM) and function redundantly with EPFL1 and EPFL2 to regulate the meristem size and leaf initiation 64 , 65 . In particular, signaling downstream of EFPL ligands and their receptors confines the WUS-CLV3 pathway to the center of the SAM by inhibiting its expression in the meristem periphery 65 .…”

Section: Resultsmentioning

confidence: 99%

“…However, two very recent studies revealed that EPFL4 and EPFL6 are also expressed in the periphery of the shoot apical meristem (SAM) and function redundantly with EPFL1 and EPFL2 to regulate the meristem size and leaf initiation 64 , 65 . In particular, signaling downstream of EFPL ligands and their receptors confines the WUS-CLV3 pathway to the center of the SAM by inhibiting its expression in the meristem periphery 65 . In this regard, it is especially interesting that AqEPFL4 came out as one of the most connected hub genes in a module that is strongly associated with s1, suggesting that our results provide further evidence for the role of the EPFLs in the meristem homeostasis.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome profiling and weighted gene co-expression network analysis of early floral development in Aquilegia coerulea

Min

¹

,

Kramer

²

2020

Sci Rep

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The earliest phases of floral development include a number of crucial processes that lay the foundation for the subsequent morphogenesis of floral organs and success in reproduction. Currently, key transcriptional changes during this developmental window have been characterized in the model species Arabidopsis thaliana, but little is known about how transcriptional dynamics change over the course of these developmental processes in other plant systems. Here, we have conducted the first in-depth transcriptome profiling of early floral development in Aquilegia at four finely dissected developmental stages, with eight biological replicates per stage. Using differential gene expression analysis and weighted gene co-expression network analysis, we identified both crucial genes whose expression changes mark the transitions between developmental stages and hub genes in co-expression modules. Our results support the potential functional conservation of key genes in early floral development that have been identified in other systems, but also reveal a number of previously unknown or overlooked loci that are worthy of further investigation. In addition, our results highlight not only the dynamics of transcriptional regulation during early floral development, but also the potential involvement of the complex, essential networks of small RNA and post-translational regulation to these developmental stages.

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“…In [ 41 ] it is discussed that a dose-dependent inhibitory effect of WUS on proliferation rates may play a role in this context. Furthermore, ERECTA signalling may be involved in confining the WUS and CLV3 expression to the meristem centre by repressing both signals [ 48 , 49 ]. These mechanisms could be compared in future extension of our model.…”

Section: Discussionmentioning

confidence: 99%

Mathematical modeling of plant cell fate transitions controlled by hormonal signals

Klawe

¹

,

Stiehl

²

,

Bastian

³

et al. 2020

View full text Add to dashboard Cite

Coordination of fate transition and cell division is crucial to maintain the plant architecture and to achieve efficient production of plant organs. In this paper, we analysed the stem cell dynamics at the shoot apical meristem (SAM) that is one of the plant stem cells locations. We designed a mathematical model to elucidate the impact of hormonal signaling on the fate transition rates between different zones corresponding to slowly dividing stem cells and fast dividing transit amplifying cells. The model is based on a simplified two-dimensional disc geometry of the SAM and accounts for a continuous displacement towards the periphery of cells produced in the central zone. Coupling growth and hormonal signaling results in a nonlinear system of reaction-diffusion equations on a growing domain with the growth rate depending on the model components. The model is tested by simulating perturbations in the level of key transcription factors that maintain SAM homeostasis. The model provides new insights on how the transcription factor HECATE is integrated in the regulatory network that governs stem cell differentiation.

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ERECTA family signaling constrainsCLAVATA3andWUSCHELto the center of the shoot apical meristem

Cited by 8 publications

References 42 publications

Mathematical modeling of plant cell fate transitions controlled by hormonal signals

Mathematical modeling of plant cell fate transitions controlled by hormonal signals

Transcriptome profiling and weighted gene co-expression network analysis of early floral development in Aquilegia coerulea

Mathematical modeling of plant cell fate transitions controlled by hormonal signals

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