Eradication of prune dwarf virus, prunus necrotic ringspot virus, and apple chlorotic leaf spot virus in sweet cherries by a combination of chemotherapy, thermotherapy, and in vitro culture

Deogratias, Jean-Marc; Dosba, F.; Lutz, A

doi:10.1080/07060668909501076

Cited by 21 publications

(14 citation statements)

References 11 publications

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“…This result complies with the abovementioned effi cacy of ribavirin at concentrations 40 -100 mg l -1 for the elimination of ACLSV (5), except that their study included only DAS-ELISA for re-testing. In addition, phytotoxicity of ribavirin observed in our study, both partially at 60 mg l -1 and at concentrations exceeding 60 mg l -1 (80 to 100 mg l -1 ) was identical to the reported phytotoxicity obtained with application of increased concentrations of ribavirin (50-100 mg l -1 ) in infected sweet cherry and P. mahaleb (5). For other host plants, e.g.…”

Section: Ribavirin Has Been Successfully Utilized For the Eliminationsupporting

confidence: 70%

“…Similarly, Parmessur and Saumtally (15) reported that chemotherapy with 10 -30 mg l -1 of ribavirin was entirely ineffective in elimination of Sugarcane yellow leaf virus and Sugarcane bacilliform virus from infected sugarcane as confi rmed by RT-PCR and PCR. Also, Deogratias et al (5) reported ineffectiveness of low ribavirin rates (10 -20 mg l -1 ) in eliminating ACLSV.…”

Section: Ribavirin Has Been Successfully Utilized For the Eliminationmentioning

confidence: 99%

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In VitroProduction ofPlum Pox Virus- Free Plums by Chemotherapy with Ribavirin

Paunović

Ružić

Vujović

et al. 2007

Biotechnology & Biotechnological Equipment

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Section: Ribavirin Has Been Successfully Utilized For the Eliminationsupporting

confidence: 70%

Section: Ribavirin Has Been Successfully Utilized For the Eliminationmentioning

confidence: 99%

In VitroProduction ofPlum Pox Virus- Free Plums by Chemotherapy with Ribavirin

Paunović

Ružić

Vujović

et al. 2007

Biotechnology & Biotechnological Equipment

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“…After chemotherapy, 100% of isolated apices developed vigorous shoots with leaves about 1 cm and multiplied in standard growth conditions. Th is is contradictory to fi ndings of other authors (Hansen, Lane 1985;Deogratias et al 1989;Cieslinska 2002Cieslinska , 2007, who reported that ribavirin starts to be phytotoxic, when used at concentrations higher than 40 mg/l. It seems that there is a species-and genotype-specifi c diff erence in the susceptibility of in vitro grown plants to higher ribavirin concentrations.…”

Section: Resultscontrasting

confidence: 56%

Results of in vitro chemotherapy of apple cv. Fragrance - Short communication

Paprštein

Sedlák

Svobodová

et al. 2013

Hort. Sci. (Prague)

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Parštein F., Sedlák J., Svobodová L., Polák J., Gadiou S., 2013. Results of in vitro chemotherapy of apple cultivar Fragrance -Short communication. Hort. Sci. (Prague),.Th e eff ect of the chemotherapy with ribavirin on the elimination of the pome fruit viruses from in vitro grown plants of infected apple cv. Fragnance has been investigated. Th e results of ELISA and RT-PCR testing proved the presence of mixed infection of Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) in the initial fi eld-grown tree of this apple cultivar. Obtained actively growing in vitro shoots with welldeveloped leaves and shoot tips were subsequently used for chemotherapy with ribavirin. Attempts to fully eliminate viruses by ribavirin in lower concentration 20 mg/l were not successful. However in vitro plants of one mericlone (FR1R20) sanitated from ASPV and ASGV, which were infected with ACLSV only after the fi rst chemotherapy cycle, were subjected to repeated treatment on medium with higher ribavirin concentration 100 mg/l. Th e success of chemotherapy with ribavirin at 100 mg/l was 76% for ACLSV elimination after the second round. In the course of both chemotherapy cycles (20 mg/l and 100 mg/l), in vitro plants did not display symptoms of phytotoxicity.

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“…Most often, the temperature of thermotherapy treatments is measured in a growth chamber. Some authors have shown that the air temperature measured in the growth chamber was 1 or 2°C (Stein et al 1991;Deogratias et al 1989) lower than that inside the test tubes. When choosing a temperature, there must be a balance between relatively low shoot mortality at higher temperatures and the high percentage of virus-free shoots (Dziedzic 2008).…”

Section: Discussionmentioning

confidence: 92%

Virus elimination from in vitro apple by thermotherapy combined with chemotherapy

Dong

Zhang

et al. 2015

Plant Cell Tiss Organ Cult

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We evaluated the effectiveness of thermotherapy at different temperatures, chemotherapy with different concentrations of ribavirin, and combinations of these two methods on virus elimination from apple. We used Malus cv. 'Xinhongjiangjun', an apple cultivar widely grown in China, infected with Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV), and Apple stem pitting virus (ASPV). The survival and regeneration of plants were evaluated, and the efficiency of virus eradication was determined by reverse-transcription polymerase chain reaction with two primer pairs for each virus. All of the plants treated with 15 and 25 lg/ml ribavirin survived, although their proliferation was slightly inhibited. Ribavirin treatments at 15 and 25 lg/ml resulted in virus elimination rates of 74.4 and 75.0 %, respectively. Higher temperatures significantly affected the growth and proliferation of plants, and almost no axillary shoots regenerated under the highest temperature (38 ± 0.5°C). The average virus elimination rate after 34-36°C treatments for 20 days was no more than 45 %. The virus elimination efficiency was enhanced by combining thermotherapy and chemotherapy treatments. A treatment combining ribavirin (25 lg/ml) and thermotherapy at 36°C (R25 ? T36) resulted in high virus eradication efficiency (95.0 %). Across all of the treatments, the average survival rate of apical shoots (176/273) was 12 % lower than that of axillary shoots (266/349); the average virus elimination efficiency was 65.3 % for apical shoots and 72.7 % for axillary shoots. The results also demonstrated that it was easier to eliminate ASPV than to eliminate ACLSV and ASGV.

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Eradication of prune dwarf virus, prunus necrotic ringspot virus, and apple chlorotic leaf spot virus in sweet cherries by a combination of chemotherapy, thermotherapy, and in vitro culture

Cited by 21 publications

References 11 publications

In VitroProduction ofPlum Pox Virus- Free Plums by Chemotherapy with Ribavirin

In VitroProduction ofPlum Pox Virus- Free Plums by Chemotherapy with Ribavirin

Results of in vitro chemotherapy of apple cv. Fragrance - Short communication

Virus elimination from in vitro apple by thermotherapy combined with chemotherapy

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