Eradication of posterior capsule opacification

Apple, David J.; Peng, Qun; Visessook, Nithi; Werner, Liliana; Pandey, Suresh K; Escobar-Gómez, Marcela; Ram, Jagat; Auffarth, Gerd U.

doi:10.1016/s0161-6420(00)00589-3

Cited by 249 publications

(45 citation statements)

References 48 publications

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“…Contrary to expectations, the incidence of capsulotomy for posterior capsule opacification (PCO) has not declined as predicted [1, 2]. Various methods to reduce the number of lens epithelial cells, mechanically or pharmacologically, have been investigated [1, 3, 4].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Growth of Lens Epithelial Cells from Cataract Patients - Association with Possible Risk Factors for Posterior Capsule Opacification

Sundelin¹,

Petersen²,

Soltanpour³

et al. 2014

TOOPHTJ

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Aim : Inter-individual differences in intrinsic proliferative capacity of lens epithelial cells may have importance for the risk of developing posterior capsule opacification (PCO) after cataract surgery. The purpose of the present study was to determine growth of human lens epithelial cells (HLEC) in culture and investigate possible associations with clinical characteristics of the donors, such as age, sex, pseudoexfoliation, uveitis and diabetes.Methods : Pieces of lens capsule and adhering lens epithelial cells were obtained through capsulorhexis at cataract surgery. Specimens were cultured in a humidified CO2-incubator using standard culture medium and 5% fetal calf serum for two weeks after which cultured cells were stained with carboxy-fluorescein diacetate succinimidyl ester. Image processing software was used to determine the area of the confluent epithelial cell layer in relation to the size of the original capsule specimen.Results : The increase in area of confluent HLEC showed a negative correlation with diabetes at the first week after surgery. Lower age and female sex showed border-line significant associations with a higher rate of cell proliferation. The presence of pseudoexfoliation in vivo did not significantly affect cell growth in culture postoperatively. Nor did installation of xylocain in the anterior chamber during surgery.Conclusion : Diabetes is associated with lower rate of proliferation of lens epithelial cells in culture. The lack of strong correlations between in vitro growth and known risk factors for PCO in the donors suggest that other factors than the proliferative capacity of the cells per se are important for PCO formation.

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Section: Introductionmentioning

confidence: 99%

In Vitro Growth of Lens Epithelial Cells from Cataract Patients - Association with Possible Risk Factors for Posterior Capsule Opacification

Sundelin¹,

Petersen²,

Soltanpour³

et al. 2014

TOOPHTJ

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“…Posterior capsular opacification (PCO) is caused by proliferation and migration of residual LEC, fibroblasts, macrophages, and iris-derived pigment cells on the posterior capsule (KAPPELHOF & VRENSEN, 1992).These residual LECs differentiate into fibroblast-like cells via EMT, resulting in fibrous plaque formation. Some authors believe that it is the anterior LECs that re-colonize the anterior capsule, and then migrate to the posterior lens capsule (WORMSTONE, 2002), while others believe that the equatorial LECs are the primary source of PCO formation (APPLE et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…It is also believed that sharp-edged IOLs and capsular rings can produce a "barrier effect" preventing the migration of these cells and, consequently, the PCO formation (APPLE et al, 2001;KUGELBERG et al, 2008;KOHNEN et al, 2008). Histopathology studies showed that a sharp capsular bend was formed at the sharp posterior optic edge of the IOLs and that the migration of lens was inhibited at this site.…”

Section: Discussionmentioning

confidence: 99%

“…The theory that the shape of the square edge is more important than the IOL materials has gained popularity and the IOLs have been modified in an attempt to effectively reduce PCO formation. Some studies have reported that a sharp posterior optic edge is the main factor in preventing PCO (KOHNEN et al, 2008) due to the "barrier effect" against lens epithelial migration (APPLE et al, 2001). …”

Section: Introductionmentioning

confidence: 99%

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Anel de tensão capsular isolado e associado à lente intraocular acrílica dobrável na opacidade de cápsula posterior após facoemulsificação em cães

et al. 2015

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“…Nd: YAG laser posterior capsulotomy is always required to restore vision [6]. However, it may increase the risk of retinal detachment, hemorrhage and lens injury, etc.…”

Section: Introductionmentioning

confidence: 99%

Rapamycin-Induced Apoptosis in HGF-Stimulated Lens Epithelial Cells by AKT/mTOR, ERK and JAK2/STAT3 Pathways

Tian

Dong

Zhou

et al. 2014

IJMS

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Hepatocyte growth factor (HGF) induced the proliferation of lens epithelial cells (LECs) and may be a major cause of posterior capsule opacification (PCO), which is the most frequent postoperative complication of cataract surgery. To date, several agents that can block LECs proliferation have been studied, but none have been used in clinic. Recently, accumulating evidence has suggested rapamycin, the inhibitor of mTOR (mammalian target of Rapamycin), was associated with the induction of apoptosis in LECs. The purpose of our study was to investigate the potential effects of rapamycin on HGF-induced LECs and the underlying mechanisms by which rapamycin exerted its actions. Using cell proliferation, cell viability and flow cytometric apoptosis assays, we found that rapamycin potently not only suppressed proliferation but also induced the apoptosis of LECs in a dose-dependent manner under HGF administration. Further investigation of the underlying mechanism using siRNA transfection revealed that rapamycin could promote apoptosis of LECs via inhibiting HGF-induced phosphorylation of AKT/mTOR, ERK and JAK2/STAT3 signaling molecules. Moreover, the forced expression of AKT, ERK and STAT3 could induce a significant suppression of apoptosis in these cells after treatment of rapamycin. Together, these findings suggested that rapamycin-induced apoptosis in HGF-stimulated LECs is accompanied by inhibition of AKT/mTOR, ERK and JAK2/STAT3 pathways, which supports its use to inhibit PCO in preclinical studies and provides theoretical foundation for future possible practice.

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Eradication of posterior capsule opacification

Cited by 249 publications

References 48 publications

In Vitro Growth of Lens Epithelial Cells from Cataract Patients - Association with Possible Risk Factors for Posterior Capsule Opacification

In Vitro Growth of Lens Epithelial Cells from Cataract Patients - Association with Possible Risk Factors for Posterior Capsule Opacification

Anel de tensão capsular isolado e associado à lente intraocular acrílica dobrável na opacidade de cápsula posterior após facoemulsificação em cães

Rapamycin-Induced Apoptosis in HGF-Stimulated Lens Epithelial Cells by AKT/mTOR, ERK and JAK2/STAT3 Pathways

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