Eradication of meticillin-resistant Staphylococcus aureus from human skin by the novel LL-37-derived peptide P10 in four pharmaceutical ointments

Nibbering, Peter H.; Göblyös, Anikó; Adriaans, A.; Cordfunke, Robert A.; Ravensbergen, Bep; Rietveld, Marion; Zwart, S.J.; Commandeur, Suzan; Leeuwen, Remko van; Haisma, Elisabeth M.; Schimmel, Kirsten J. M.; Hartigh, Jan den; Drijfhout, Jan W.; Ghalbzouri, Abdoelwaheb El

doi:10.1016/j.ijantimicag.2019.07.014

Cited by 11 publications

(5 citation statements)

References 22 publications

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“…For instance, Hylin-a1 peptide, derived from the skin of the frog Heleioporus albopunctatus, was recently reported by our group as a potent antimicrobial agent capable of inhibiting S. aureus invasion and modulating levels of inflammatory cytokines [16]. Furthermore, the interest in the identification and design of new molecules with greater potency, selectivity, and cost-effectiveness has been driven by the approval of some AMPs for human use [26,[29][30][31]. Among Staphylococci, S. aureus is of most clinical concern because of its great biofilm-forming capacity on biomaterials and medical devices, the production of several toxins, and the ability to evolve resistance mechanisms [22,32].…”

Section: Discussionmentioning

confidence: 99%

Oreoch-1: A Peptide from Oreochromis niloticus as a Potential Tool against Staphylococci

Palma,

Chianese,

Panico

et al. 2023

Pathogens

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Staphylococci, including Staphylococcus aureus and Staphylococcus epidermidis, are important human pathogens associated with potentially life-threatening infections. Their great biofilm-producing ability and the development of resistance mechanisms often account for therapeutic failure. Hence, the scientific community has devoted intensive efforts to the development of antimicrobial compounds active against both planktonic and sessile bacterial populations. Contextually, antimicrobial peptides (AMPs) are natural peptides produced by the innate immunity of every organism, representing a potential new therapeutic solution against human microbial pathogens. Our work focused on the in vitro activity of Oreoch-1, an AMP from the gills of Nile tilapia (Oreochromis niloticus), against standard and clinical S. aureus and S. epidermidis strains. Firstly, the cytotoxicity profile of Oreoch-1 was determined in human colon carcinoma cells. Secondly, its antibacterial spectrum was explored against staphylococcal strains to set up the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). Our results highlighted an antibacterial activity in the range 6.25–25 μM, with a general bacteriostatic effect. Therefore, the biofilm-inhibitory property was assessed against S. aureus ATCC 25923 and S. epidermidis ATCC 35984, indicating a significant reduction in S. aureus biomass at sub-MIC concentrations. Overall, our study indicates Oreoch-1 as a promising new therapeutic weapon against staphylococcal infections.

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Section: Discussionmentioning

confidence: 99%

Oreoch-1: A Peptide from Oreochromis niloticus as a Potential Tool against Staphylococci

Palma,

Chianese,

Panico

et al. 2023

Pathogens

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“…A formulation containing magainin and the surfactant chemical enhancer N -lauroylsarcosine in 50% ethanol could synergistically increase skin permeability to fluorescein by 47-fold. There are many other transdermal delivery systems for MDPs, having also been incorporated into cream 299 , ointment 300 , and wafer 301 , which have made good attempts for their clinical application.…”

Section: Mdps-based Formulations and Their Applicationsmentioning

confidence: 99%

Membrane-disruptive peptides/peptidomimetics-based therapeutics: Promising systems to combat bacteria and cancer in the drug-resistant era

Lin

Chi

Yan

et al. 2021

Acta Pharmaceutica Sinica B

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Membrane-disruptive peptides/peptidomimetics (MDPs) are antimicrobials or anticarcinogens that present a general killing mechanism through the physical disruption of cell membranes, in contrast to conventional chemotherapeutic drugs, which act on precise targets such as DNA or specific enzymes. Owing to their rapid action, broad-spectrum activity, and mechanisms of action that potentially hinder the development of resistance, MDPs have been increasingly considered as future therapeutics in the drug-resistant era. Recently, growing experimental evidence has demonstrated that MDPs can also be utilized as adjuvants to enhance the therapeutic effects of other agents. In this review, we evaluate the literature around the broad-spectrum antimicrobial properties and anticancer activity of MDPs, and summarize the current development and mechanisms of MDPs alone or in combination with other agents. Notably, this review highlights recent advances in the design of various MDP-based drug delivery systems that can improve the therapeutic effect of MDPs, minimize side effects, and promote the co-delivery of multiple chemotherapeutics, for more efficient antimicrobial and anticancer therapy.

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“…161 Staphefekt SA.100, a recombinant endolysin produced by bacteriophages, has been successfully used to treat SA folliculitis in three cases. 162 Staphefekt SA.100 demonstrated activity against SA and led to a Wang et al 174 Ong et al 27 Nibbering et al 175 Human beta-defensin 2 (HBD-2) Decreases SA in an in vitro reconstructed human epidermis model SA infection induces HBD-2 expression in human skin infection Decreased HBD-2 expression in AD human skin biopsy samples Treatment of AD human skin explants with anti-IL-10 augmented HBD-2 expression Superficial skin injury induces increased secretion in AD lesional and nonlesional skin vs controls Ong et al 27 Nomura et al 176 Howell et al 177 Rosignoli et al 100 Harder et al 178 Zanger et al 179 Human beta-defensin 3 (HBD-3) Keratinocytes secrete HBD-3 onto SA to kill SA in human skin explants SA infection induces HBD-3 expression and HBD-3 levels are inversely associated with human skin infection severity Keratinocytes isolated from AD human skin were unable to mobilize HBD-3 to kill SA Decreased HBD-3 expression in AD human skin biopsy samples Treatment of AD human skin explants with antibodies against Th2 cytokines had augmented HBD-3 expression Superficial skin injury induces increased secretion in AD lesional and nonlesional skin vs controls Kisich et al 180 Nomura et al 176 Kisich et al 181 Howell et al 182 Zanger et al 179 S 178 Zanger et al 186 decreased EASI score in a double-blind, vehicle-controlled, RCT of 100 adult AD patients, but these differences were not significant. 163…”

Section: Bacteriophagesmentioning

confidence: 99%

Updated understanding of Staphylococcus aureus in atopic dermatitis: From virulence factors to commensals and clonal complexes

Hwang

Thompson

Jaros

et al. 2021

Experimental Dermatology

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Atopic dermatitis (AD) is an inflammatory dermatosis with a pathogenesis believed to be due to a combination of genetic, immunologic and environmental factors that affects up to 5% of adults and 20% of children worldwide. 1,2 Common genetic factors include polymorphisms in the filaggrin structural protein, interleukin (IL)-4 receptor and vitamin D receptor. [3][4][5] AD is driven by multiple immune pathways, most commonly with activation of Th2 and Th22 T-cell subsets. 6 Crosstalk between commensals and the host immune system modulates adaptive and innate immune responses in AD. 7 Staphylococcus aureus (SA) in AD lesions and surrounding normal skin was first noted by Leyden et al. in the 1970s; since then, the understanding of its role in AD has drastically evolved. 8,9 SA is a well-established exacerbator of AD; it is frequently isolated from the skin of AD patients and increased SA density is found during flares. 10 A meta-analysis in 2016 reported a pooled prevalence of SA colonization among AD patients of 70% in lesional skin, 39% in non-lesional skin and 62% in the nose. 11 More recently, Kong et al 12 found that AD treatments and flares were closely associated with shifts in the cutaneous microbial diversity. While current evidence has established that SA proliferates during flares and plays a role in the cycle of epidermal breakdown and inflammation, a true causal relationship has not been established. Herein, we will summarize the

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Eradication of meticillin-resistant Staphylococcus aureus from human skin by the novel LL-37-derived peptide P10 in four pharmaceutical ointments

Cited by 11 publications

References 22 publications

Oreoch-1: A Peptide from Oreochromis niloticus as a Potential Tool against Staphylococci

Oreoch-1: A Peptide from Oreochromis niloticus as a Potential Tool against Staphylococci

Membrane-disruptive peptides/peptidomimetics-based therapeutics: Promising systems to combat bacteria and cancer in the drug-resistant era

Updated understanding of Staphylococcus aureus in atopic dermatitis: From virulence factors to commensals and clonal complexes

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