Eradication of adenovirus E1-induced tumors by E1A-specific cytotoxic T lymphocytes

Kast, W. Martin; Offringa, Rienk; Peters, Peter J.; Voordouw, A. C.; Meloen, Rob H.; Eb, A. J. Van Der; Melief, Cornelis J.M.

doi:10.1016/0092-8674(89)90006-8

Cited by 277 publications

(142 citation statements)

References 43 publications

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“…After culture, 50 /¿I supernatant were collected. We used EL4 target cells (thymoma cell line of B6 origin) (Gorer, 1950) resuspended in culture medium supplemented with or without 20 fiM S9 or 20 ¡jlM 10 amino acid long ade novirus type 5 E1A peptide (A10: amino acid sequence 234-243) (Kast et al, 1989(Kast et al, , 1991b) be fore adding them 1:1 (v/v) to the effector cells. The percentage specific 5lCr release was calcu lated by the formula: % specific lysis = 100 X [(cpm experimental well -background 51Cr reIease)/(cpm 2% Triton X-100 release -back ground 51Cr release)].…”

Section: Primary Responsementioning

confidence: 99%

A rapid isolation procedure for dendritic cells from mouse spleen by centrifugal elutriation

Ossevoort

Toes

Bruijn

et al. 1992

Journal of Immunological Methods

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The standard isolation procedure for antigen presenting dendritic cells (DC) takes 2 days and includes selective adherence to tissue culture plates which may lead to the activation of these cells. This report describes the isolation of DC by centrifugal elutriation (CE). Murine spleen cells were separated on the basis of size and density into 7 CE fractions. This method took 90 min. Ceils from each CE fraction were characterized by fluorescence activated cell sorter (FACS) analysis and their antigen presenting cell (APC) activity was determined by a secondary Sendai virus specific T cell proliferation assay. CE fraction 5 contained most of the DC with a concentration of 6-10% , representing an approximately 15-fold enrichment compared to unseparated spleen cells ( < 1 % DC). This CE fraction also exhibited the highest APC activity, which was almost completely abolished after depletion of DC by treatment with monoclonal antibody 33D1 (DC-marker) and complement. Further enrichment of CE fraction 5 by discontinuous density gradient centrifugation resulted in a cell population containing 35-55% 33Dl-positive cells with similar characteristics as DC isolated by the standard procedure, such as the capacity to induce a primary viral peptide specific CTL response. Two-color FACS analysis showed an increase in MHC expression on 33Dl-positive cells of CE fraction 5 after 18 h culture involving cell adhesion to a similar level as the M HC expression on DC isolated by the standard procedure. During this same period their morphology changed from a round to a dendritic appearance. In conclusion, our results indicate that CE is well suited for isolating DC more rapidly and without activation of these cells by adherence, a process which readily occurs in the standard isolation procedure.

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Section: Primary Responsementioning

confidence: 99%

A rapid isolation procedure for dendritic cells from mouse spleen by centrifugal elutriation

Ossevoort

Toes

Bruijn

et al. 1992

Journal of Immunological Methods

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“…2 Moreover, (clinical) adoptive immunotherapy studies demonstrated that the transfer of viral or tumor-reactive CD8 + T lymphocytes can result in effective antiviral and antitumor responses, respectively. [3][4][5][6][7] In addition, vaccination studies using MHC class I immuno-dominant peptides have shown that protective antitumor immunity can be generated. 8,9 On the other hand, the important role of CD4 + T lymphocytes in the induction and persistence of effective immunity was demonstrated in studies using CMV-specific CTL clones as well as CD4 knockout mice.…”

Section: Introductionmentioning

confidence: 99%

Redirecting human CD4+ T lymphocytes to the MHC class I-restricted melanoma antigen MAGE-A1 by TCR αβ gene transfer requires CD8α

Willemsen¹,

Ronteltap²,

Heuveling³

et al. 2004

Gene Ther

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“…T lymphocytes (CTL) play an important role in the control of viral infections (Yap et al, 1978;Zinkemagel et al, 1979) and tnmour cell growth (Kast et al, 1989;Greenberg, 1991). For these reasons, the understanding of how CTL are induced in viva is of paramount importance both for vaccine design and cancer therapy.…”

Section: Cytotoxicmentioning

confidence: 99%

In vivo cytotoxic T-lymphocyte induction may take place via CD8+ T helper lymphocytes

Lasarte

Sarobe

Prìeto

et al. 1995

Research in Immunology

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Immunization of mice with peptide constructs, consisting of a determinant recognized by T cytotoxic cells colinearly linked to a determinant recognized by T helper cells (TD,-TD 1 ?was able to induce cytotoxic T lymphocytes in vivo. Interestingly, this induction cou d be achieved in the absence of adjuvant in non-depleted as well as in CD4+-cell-depleted BALB/c mice. In the latter case, induction took place simultaneously with the activation of CD8+ T helper cells specific for a TD, contained within the sequence of the TD, RIQRGPGRAPVTIGK from the immunodommant V3 loop of HIV1 gp120. The possible implications of these findings in HIV infection and AIDS disease are discussed.

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Eradication of adenovirus E1-induced tumors by E1A-specific cytotoxic T lymphocytes

Cited by 277 publications

References 43 publications

A rapid isolation procedure for dendritic cells from mouse spleen by centrifugal elutriation

A rapid isolation procedure for dendritic cells from mouse spleen by centrifugal elutriation

Redirecting human CD4+ T lymphocytes to the MHC class I-restricted melanoma antigen MAGE-A1 by TCR αβ gene transfer requires CD8α

In vivo cytotoxic T-lymphocyte induction may take place via CD8+ T helper lymphocytes

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