Era of TB elimination: Growing need to understand diversities of Mycobacterium tuberculosis lineages!

Tejashree, A; Modesti, Pietro Amedeo; Maruthachalam, Karthik; Nirmala,; Azeem, Abdul; Reddy, Raveendra H R; Ravichandra, Chinnappareddy; Nagaraja, Sharath Burugina

doi:10.1016/j.ijtb.2021.03.013

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…A more granular analysis of the WGS to identify genetic features of the pan-genomes of the isolates from DM vs. non-DM TB patients may reveal features associated with DM or non-DM patients. Table 5 shows studies determining lineages and spoligotypes distributed across the world and specifically in India [ 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 ]. An overview of various studies conducted in different regions around the world to determine the predominant lineages and spoligotypes of Mycobacterium tuberculosis , offers valuable insights into the distribution and diversity of the strains in different populations, and also raises several points for critical discussion.…”

Section: Discussionmentioning

confidence: 99%

Whole-Genome Sequencing of Mycobacterium tuberculosis Isolates from Diabetic and Non-Diabetic Patients with Pulmonary Tuberculosis

Shankaregowda

Sumana

et al. 2023

Microorganisms

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The association of tuberculosis and type 2 diabetes mellitus has been a recognized re-emerging challenge in management of the convergence of the two epidemics. Though much of the literature has studied this association, there is less knowledge in the field of genetic diversities that might occur in strains infecting tuberculosis patients with and without diabetes. Our study focused on determining the extent of diversity of genotypes of Mycobacterium tuberculosis in both these categories of patients. We subjected 55 M. tuberculosis isolates from patients diagnosed with pulmonary TB with and without type 2 diabetes mellitus to whole-genome sequencing on Illumina Hi Seq platform. The most common lineage identified was lineage 1, the Indo-Oceanic lineage (n = 22%), followed by lineage 4, the Euro-American lineage (n = 18, 33%); lineage 3, the East-African Indian lineage (n = 13, 24%); and lineage 2, the East-Asian lineage (n = 1, 2%). There were no significant differences in the distribution of lineages in both diabetics and non-diabetics in the South Indian population, and further studies involving computational analysis and comparative transcriptomics are needed to provide deeper insights.

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Section: Discussionmentioning

confidence: 99%

Whole-Genome Sequencing of Mycobacterium tuberculosis Isolates from Diabetic and Non-Diabetic Patients with Pulmonary Tuberculosis

Shankaregowda

Sumana

et al. 2023

Microorganisms

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Spoligotyping of the Mycobacterium tuberculosis complex using on-Chip PCR

Lapa

Kuźmin

Сhernousova

et al. 2022

Journal of Applied Microbiology

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Aims The aim of this study was to develop a rapid PCR-based method for spoligotyping of Mycobacteria in the microarray format and to compare it to conventional spoligotyping by hybridization. Methods and results The method employs the on-Chip PCR technique with primers specific for 43 spacers that separate direct repeats (DRs) in the DR region of mycobacterial DNA. The primers were immobilized on gel-based microarrays, and PCR was performed directly on the chips. The PCR fluorescence images were acquired and processed using a portable fluorescence analyzer equipped with dedicated software. Analysis takes 1.5–2 hours and can be carried out on clinical samples without additional handling. The analytical sensitivity of the method was 103 copies of target DNA. The spoligotyping results of 51 samples produced by the proposed method and by conventional reverse hybridization approach were in full concordance. Conclusions High throughput capacity, computerized data analysis, compact equipment, and reliable results make the on-Chip PCR an attractive alternative to intra- and interspecific spoligotyping of Mycobacterium tuberculosis complex bacteria. Significance and Impact of Study Fast microarray-based spoligotyping technique using on-Chip PCR was developed.

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Era of TB elimination: Growing need to understand diversities of Mycobacterium tuberculosis lineages!

Cited by 2 publications

References 11 publications

Whole-Genome Sequencing of Mycobacterium tuberculosis Isolates from Diabetic and Non-Diabetic Patients with Pulmonary Tuberculosis

Whole-Genome Sequencing of Mycobacterium tuberculosis Isolates from Diabetic and Non-Diabetic Patients with Pulmonary Tuberculosis

Spoligotyping of the Mycobacterium tuberculosis complex using on-Chip PCR

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