Er-Xian decoction attenuates ovariectomy-induced osteoporosis by modulating fatty acid metabolism and IGF1/PI3K/AKT signaling pathway

Ma, Yujie; Hu, Jing; Song, Changheng; Li, Pei; Cheng, Yin; Wang, Yuhan; Liu, Haixia; Chen, Yanjing; Zhang, Zhiguo

doi:10.1016/j.jep.2022.115835

Cited by 5 publications

(5 citation statements)

References 45 publications

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“…Erxian Decoction (EXD): The main active ingredients are Monotropein, mangiferin, berberine hydrochloride, ferulic acid, curculigoside, and icariin ( 45 ). EXD exerts estrogen-like effects and is effective in reducing bone loss by increasing BMD and improving bone microarchitecture as well as restoring serum levels of the osteoblast-secreted (OCN) protein ( 44 ); it also enhances the proliferation rate of osteoblast-like UMR-0 cells in rats ( 46 ), and promotes the proliferation of mouse embryonic osteoblast precursor cells, Mc3t3-e1 cells, and osteogenic differentiation by regulating the BK channel ( 47 ).…”

Section: Discussionmentioning

confidence: 99%

“…EXD also activates the Insulin-like growth factor 1 receptor/Phosphatidylinositol 3-kinase/Protein kinase B(IGF1/PI3K/AKT) signaling pathway by increasing serum IGF1 concentration and tibial Insulin-like growth factor 1 receptor(IGF1R), PI3K, and AKT expression, thereby promoting osteogenic differentiation and proliferation of osteoblasts. Moreover, it regulates the concentration of medium and long-chain free fatty acid (MLCFA) and inhibits Stearoyl-CoA desaturase 1 (SCD1) activity, restoring disturbed lipid metabolism in adipose tissue and promoting fatty acid synthetase expression through activation of the IGF1/PI3K/AKT signaling pathway to alleviate osteoporosis symptoms ( 45 ).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, key active ingredients such as psoralen, isostatin, and sulforaphane activate adenylate cyclase to promote the biosynthesis of various sex steroid hormones, elevating the levels of 17b-estradiol, luteinizing hormone, 12a-hydroxyprogesterone, and androstenedione (43). Capsule also improves the bone growth factor bone morphogenetic protein 2 (BMP-2) expression in osteoporotic rats with fractures, enhances insulin-like growth factor-1 (IGF-1) expression, and promotes bone (45). EXD exerts estrogen-like effects and is effective in reducing bone loss by increasing BMD and improving bone microarchitecture as well as restoring serum levels of the osteoblast-secreted (OCN) protein (44); it also enhances the proliferation rate of osteoblast-like UMR-0 cells in rats (46), and promotes the proliferation of mouse embryonic osteoblast precursor cells, Mc3t3-e1 cells, and osteogenic differentiation by regulating the BK channel (47).…”

Section: Active Ingredients and Mechanisms Of Action Of Tcmmentioning

confidence: 99%

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Osteoporosis guidelines on TCM drug therapies: a systematic quality evaluation and content analysis

Zhang,

Li,

Xie

et al. 2024

Front. Endocrinol.

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ObjectiveThe aims of this study were to evaluate the quality of osteoporosis guidelines on traditional Chinese medicine (TCM) drug therapies and to analyze the specific recommendations of these guidelines.MethodsWe systematically collected guidelines, evaluated the quality of the guidelines using the Appraisal of Guidelines Research and Evaluation (AGREE) II tool, and summarized the recommendations of TCM drug therapies using the Patient-Intervention-Comparator-Outcome (PICO) model as the analysis framework.Results and conclusionsA total of 20 guidelines were included. Overall quality evaluation results revealed that four guidelines were at level A, four at level B, and 12 at level C, whose quality needed to be improved in the domains of “stakeholder involvement”, “rigor of development”, “applicability” and “editorial independence”. Stratified analysis suggested that the post-2020 guidelines were significantly better than those published before 2020 in the domains of “scope and purpose”, “stakeholder involvement” and “editorial independence”. Guidelines with evidence systems were significantly better than those without evidence systems in terms of “stakeholder involvement”, “rigor of development”, “clarity of presentation” and “applicability”. The guidelines recommended TCM drug therapies for patients with osteopenia, osteoporosis and osteoporotic fracture. Recommended TCM drugs were mainly Chinese patent medicine alone or combined with Western medicine, with the outcome mainly focused on improving bone mineral density (BMD).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Active Ingredients and Mechanisms Of Action Of Tcmmentioning

confidence: 99%

See 1 more Smart Citation

Osteoporosis guidelines on TCM drug therapies: a systematic quality evaluation and content analysis

Zhang,

Li,

Xie

et al. 2024

Front. Endocrinol.

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“…5 The main potential biological pathways were analyzed by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Functional terms and pathways were visually analyzed using R 4.0.2 (R Foundation, Vienna, Austria) ( 16 ).…”

Section: Methodsmentioning

confidence: 99%

Simiao Wan and its ingredients alleviate type 2 diabetes mellitus via IRS1/AKT2/FOXO1/GLUT2 signaling

Xia

Xu²,

et al. 2023

Front. Nutr.

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BackgroundType 2 diabetes mellitus (T2DM) is a metabolic disease. Simiao Wan (SMW) is a commonly used clinical drug for hyperuricemia treatment. SMW has been confirmed to improve insulin resistance and is expected to be a novel hypoglycemic agent. However, the hypoglycemic bioactive ingredients and mechanisms of action of SMW are unclear.ObjectiveTo explore the hypoglycemic effects and reveal the mechanisms of SMW and bioactive ingredients (SMW-BI).Study design and methodsThe hypoglycemic effects of SMW and SMW-BI were verified in a mouse model of T2DM induced by streptozotocin (STZ) and a high-fat and high-sugar diet (HFSD). Network pharmacology was used to predict the mechanisms of SMW and SMW-BI. Histological analysis and real-time quantitative polymerase chain reaction (RT-qPCR) verified network pharmacology results. RT-qPCR results were further verified by immunofluorescence (IFC) and molecular docking. The correlation between proteins and biochemical indicators was analyzed by Spearman’s correlation.ResultsChlorogenic acid, phellodendrine, magnoflorine, jateorhizine, palmatine, berberine, and atractydin were identified as SMW-BI. After 8 weeks of treatment, SMW and SMW-BI decreased the levels of fasting blood glucose (FBG), total cholesterol (TC), triacylglycerols (TG) and low-density lipoprotein cholesterol (LDL-C), increased the level of high-density lipoprotein cholesterol (HDL-C), alleviated weight loss, and increased serum insulin levels in T2DM mice. In addition, SMW and SMW-BI improved hepatocyte morphology in T2DM mice, decreased the number of adipocytes, and increased liver glycogen. Network pharmacological analysis indicated that SMW and SMW-BI may exert hypoglycemic by regulating insulin receptor substrate 1 (IRS1)/RAC-beta serine/threonine-protein kinase (AKT2)/forkhead box protein O1 (FOXO1)/glucose transporter type 2 (GLUT2) signaling. Moreover, correlation analysis showed that SMW and SMW-BI were associated with activation of IRS1, AKT2, and GLUT2, and inhibiting FOXO1. RT-qPCR revealed that SMW and SMW-BI could increase levels of IRS1, AKT2, and GLUT2 in the livers of T2DM mice and lower the level of FOXO1. Furthermore, immunofluorescence analysis showed that FOXO1 expression in the livers of T2DM mice decreased after oral administration of SMW and SMW-BI. Furthermore, molecular docking showed that SMW-BI could bind directly to IRS1 and AKT2.ConclusionSMW and SMW-BI are potential hypoglycemic drugs that alleviate T2DM by regulating IRS1/AKT2/FOXO1 signaling. Our study provides a research idea for screening the bioactive ingredients in traditional Chinese medicine (TCM).

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“…Moreover, EXD, which has been extensively used for the treatment of OP ( 102 ), can improve the bone microstructure and deterioration of bone loss in the OVX-induced OP model through lipid metabolism and the IGF1/PI3K/AKT pathway ( 103 ). BSTLD treatment results in a significant promotion in angiogenesis in OVX-induced OP rats, which could be associated with the promotion of HIF-1α/VEGF-regulated angiogenesis signal transduction and inhibition of the RANKL/OPG ratio ( 53 ).…”

Section: Anti-op Effects Of Tcm and Plant-based Natural Compoundsmentioning

confidence: 99%

Mechanisms of action and synergetic formulas of plant-based natural compounds from traditional Chinese medicine for managing osteoporosis: a literature review

Zhou,

Shen,

Zhang

et al. 2023

Front. Med.

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Osteoporosis (OP) is a systemic skeletal disease prevalent in older adults, characterized by substantial bone loss and deterioration of microstructure, resulting in heightened bone fragility and risk of fracture. Traditional Chinese Medicine (TCM) herbs have been widely employed in OP treatment owing to their advantages, such as good tolerance, low toxicity, high efficiency, and minimal adverse reactions. Increasing evidence also reveals that many plant-based compounds (or secondary metabolites) from these TCM formulas, such as resveratrol, naringin, and ginsenoside, have demonstrated beneficial effects in reducing the risk of OP. Nonetheless, the comprehensive roles of these natural products in OP have not been thoroughly clarified, impeding the development of synergistic formulas for optimal OP treatment. In this review, we sum up the pathological mechanisms of OP based on evidence from basic and clinical research; emphasis is placed on the in vitro and preclinical in vivo evidence-based anti-OP mechanisms of TCM formulas and their chemically active plant constituents, especially their effects on imbalanced bone homeostasis regulated by osteoblasts (responsible for bone formation), osteoclasts (responsible for bone resorption), bone marrow mesenchymal stem cells as well as bone microstructure, angiogenesis, and immune system. Furthermore, we prospectively discuss the combinatory ingredients from natural products from these TCM formulas. Our goal is to improve comprehension of the pharmacological mechanisms of TCM formulas and their chemically active constituents, which could inform the development of new strategies for managing OP.

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Er-Xian decoction attenuates ovariectomy-induced osteoporosis by modulating fatty acid metabolism and IGF1/PI3K/AKT signaling pathway

Cited by 5 publications

References 45 publications

Osteoporosis guidelines on TCM drug therapies: a systematic quality evaluation and content analysis

Osteoporosis guidelines on TCM drug therapies: a systematic quality evaluation and content analysis

Simiao Wan and its ingredients alleviate type 2 diabetes mellitus via IRS1/AKT2/FOXO1/GLUT2 signaling

Mechanisms of action and synergetic formulas of plant-based natural compounds from traditional Chinese medicine for managing osteoporosis: a literature review

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