Er3+-to-dye energy transfer in DNA-coated core and core/shell/shell upconverting nanoparticles with 980 nm and 808 nm excitation of Yb3+and Nd3+

Francés‐Soriano, Laura; Peruffo, Nicola; Natile, Marta Maria; Hildebrandt, Niko

doi:10.1039/c9an02532d

Cited by 20 publications

(20 citation statements)

References 60 publications

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“…The NIR excitation and the total elimination of the autofluorescence are desirable features for bioassays also because they can simplify sample processing and allow to perform assays in highly colorful, highly absorbing, or autofluorescent samples such as whole blood or tissue. [20] Up to now, UCNPs have been exploited as donors in RET with numerous types of acceptors, such as organic dyes (e.g., Rose Bengal, [21,22] Cy3.5, [23] rhodamine B [24] a DBD-6 small organic dye, [25] and others [26] ), but also with inorganic NPs (quantum dots, [16,[27][28][29][30][31][32][33][34][35][36] gold NPs [14] or even lanthanides themselves [37] ). Wide range of possible UCNP RET applications have been also demonstrated, [15,38,39] for example in solar-energy H 2 generation, [40] photocatalysis, [33,34,[41][42][43][44] (bio) detection [22,[31][32][33]35,[45][46][47] or bioimaging.…”

Section: Introductionmentioning

confidence: 99%

“…[27,32,67] Due to inverse 6th power distance dependence (defined by dipole-dipole interaction scheme of RET mechanism) the increased Di to Aj distances can result in strongly reduced RET efficiency (Equation S1, Supporting Information). Nevertheless, the great advantage of UCNPs is the possibility to design core-(multiple) shell NPs and intentionally select most appropriate chemical composition architectures aiming to optimally satisfy the requirements of RET based biosensing, [26,[68][69][70] provide additional functionality [71][72][73][74][75][76] or pre-defined spectral properties. [60,61,73,[77][78][79] Despite numerous studies have been undertaken, the subject is far from being well understood.…”

Section: Introductionmentioning

confidence: 99%

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Engineering the Compositional Architecture of Core‐Shell Upconverting Lanthanide‐Doped Nanoparticles for Optimal Luminescent Donor in Resonance Energy Transfer: The Effects of Energy Migration and Storage

Pilch-Wróbel

Kotulska

Lahtinen

et al. 2022

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Förster Resonance Energy Transfer (FRET) between single molecule donor (D) and acceptor (A) is well understood from a fundamental perspective and is widely applied in biology, biotechnology, medical diagnostics, and bio‐imaging. Lanthanide doped upconverting nanoparticles (UCNPs) have demonstrated their suitability as alternative donor species. Nevertheless, while they solve most disadvantageous features of organic donor molecules, such as photo‐bleaching, spectral cross‐excitation, and emission bleed‐through, the fundamental understanding and practical realizations of bioassays with UCNP donors remain challenging. Among others, the interaction between many donor ions (in donor UCNP) and many acceptors anchored on the NP surface and the upconversion itself within UCNPs, complicate the decay‐based analysis of D‐A interaction. In this work, the assessment of designed virtual core‐shell NP (VNP) models leads to the new designs of UCNPs, such as …@Er, Yb@Er, Yb@YbEr, which are experimentally evaluated as donor NPs and compared to the simulations. Moreover, the luminescence rise and decay kinetics in UCNP donors upon RET is discussed in newly proposed disparity measurements. The presented studies help to understand the role of energy‐transfer and energy migration between lanthanide ion dopants and how the architecture of core‐shell UCNPs affects their performance as FRET donors to organic acceptor dyes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Engineering the Compositional Architecture of Core‐Shell Upconverting Lanthanide‐Doped Nanoparticles for Optimal Luminescent Donor in Resonance Energy Transfer: The Effects of Energy Migration and Storage

Pilch-Wróbel

Kotulska

Lahtinen

et al. 2022

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“…Moreover, the recorded steady-state photoluminescence spectrum of the UC@CB[7] upon 975-nm excitation displayed the typical sharp emission bands of the Er 3+ cations, i.e., ca. 520 nm ( 2 H 11/2 , 4 S 3/2 → 4 I 15/2 ), 540 nm ( 4 S 3/2 → 4 I 15/2 ), and 660 nm ( 4 F 9/2 → 4 I 15/2 ) [43].…”

Section: Discussionmentioning

confidence: 99%

Initial Biological Assessment of Upconversion Nanohybrids

et al. 2021

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Nanoparticles for medical use should be non-cytotoxic and free of bacterial contamination. Upconversion nanoparticles (UCNPs) coated with cucurbit[7]uril (CB[7]) made by combining UCNPs free of oleic acid, here termed bare UCNPs (UCn), and CB[7], i.e., UC@CB[7] nanohybrids, could be used as photoactive inorganic-organic hybrid scaffolds for biological applications. UCNPs, in general, are not considered to be highly toxic materials, but the release of fluorides and lanthanides upon their dissolution may cause cytotoxicity. To identify potential adverse effects of the nanoparticles, dehydrogenase activity of endothelial cells, exposed to various concentrations of the UCNPs, was determined. Data were verified by measuring lactate dehydrogenase release as the indicator of loss of plasma membrane integrity, which indicates necrotic cell death. This assay, in combination with calcein AM/Ethidium homodimer-1 staining, identified induction of apoptosis as main mode of cell death for both particles. The data showed that the UCNPs are not cytotoxic to endothelial cells, and the samples did not contain endotoxin contamination. Higher cytotoxicity, however, was seen in HeLa and RAW 264.7 cells. This may be explained by differences in lysosome content and particle uptake rate. Internalization of UCn and UC@CB[7] nanohybrids by cells was demonstrated by NIR laser scanning microscopy.

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“… 56–58 Lanthanide based Resonance Energy Transfer (RET) with lanthanide ion donor doped inorganic nanoparticles has been demonstrated with a wide range of acceptors, such as organic dyes ( e.g. rose bengal, 59,60 cyanine Cy3.5, 61 rhodamine B, 62 a DBD-6 small organic dye 63 and others 64 ) and also with inorganic nanoparticle acceptors, such as quantum dots, 9,10,65–73 gold nanoparticles 74 or even lanthanides themselves. 75 For the sake of clarity we will use the term FRET to denote resonant energy transfer between molecules, while RET acronyms will be used to discuss cases in which Ln 3+ ions are donors.…”

Section: Introductionmentioning

confidence: 99%

Enhancing FRET biosensing beyond 10 nm with photon avalanche nanoparticles

2020

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Er³⁺-to-dye energy transfer in DNA-coated core and core/shell/shell upconverting nanoparticles with 980 nm and 808 nm excitation of Yb³⁺and Nd³⁺

Abstract: FRET from upconversion nanoparticles to dyes using 980 nm and 808 nm excitation.

Cited by 20 publications

References 60 publications

Engineering the Compositional Architecture of Core‐Shell Upconverting Lanthanide‐Doped Nanoparticles for Optimal Luminescent Donor in Resonance Energy Transfer: The Effects of Energy Migration and Storage

Engineering the Compositional Architecture of Core‐Shell Upconverting Lanthanide‐Doped Nanoparticles for Optimal Luminescent Donor in Resonance Energy Transfer: The Effects of Energy Migration and Storage

Initial Biological Assessment of Upconversion Nanohybrids

Enhancing FRET biosensing beyond 10 nm with photon avalanche nanoparticles

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