ER-resident proteins PDR2 and LPR1 mediate the developmental response of root meristems to phosphate availability

Ticconi, Carla A.; Lucero, Rocco D.; Sakhonwasee, Siriwat; Adamson, Aaron W.; Creff, Audrey; Nussaume, Laurent; Desnos, Thierry; Abel, Steffen

doi:10.1073/pnas.0901778106

Cited by 228 publications

(254 citation statements)

References 42 publications

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“…Current studies have suggested that this effect is, at least in part, associated with increased Fe accumulation in root meristems when plants are grown under low P (Hirsch et al, 2006;Ward et al, 2008;Zheng et al, 2009). In agreement with a possible Fe-induced repression of the PR under low P, it has been shown that PR elongation can be restored by lowering the Fe concentrations in the P-deficient medium (Ward et al, 2008;Ticconi et al, 2009). Despite being essential for plant growth and development, an overload of Fe can produce highly reactive hydroxyl radicals that have toxic effects in cells (Graf et al, 1984).…”

Section: The Effect Of Nutrient Availability On Root Lengthsupporting

confidence: 54%

“…of PR elongation under low P has been associated with reduced cell elongation and the loss of cell division activity in the PR apical meristem (Sanchez-Calderon et al, 2005;Ticconi et al, 2009). Current studies have suggested that this effect is, at least in part, associated with increased Fe accumulation in root meristems when plants are grown under low P (Hirsch et al, 2006;Ward et al, 2008;Zheng et al, 2009).…”

Section: The Effect Of Nutrient Availability On Root Lengthmentioning

confidence: 98%

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Plasticity of the Arabidopsis Root System under Nutrient Deficiencies

et al. 2013

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Section: The Effect Of Nutrient Availability On Root Lengthsupporting

confidence: 54%

Section: The Effect Of Nutrient Availability On Root Lengthmentioning

confidence: 98%

Plasticity of the Arabidopsis Root System under Nutrient Deficiencies

et al. 2013

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“…By contrast, the phosphate deficiency responses2 (pdr2) mutant displays a hypersensitive response of root meristems to Pi deficiency (Ticconi et al, 2004). The PDR2 gene encodes a P5-type ATPase that is localized in the endoplasmic reticulum and interacts with LPR1 genetically in mediating primary root growth under Pi deficiency (Ticconi et al, 2009). Auxin (López-Bucio et al, 2002;Nacry et al, 2005;Pérez-Torres et al, 2008), GAs (Jiang et al, 2007), and ethylene (Borch et al, 1999;Ma et al, 2003;Yu et al, 2012) are also involved in the control of Pi deficiency-mediated remodeling of RSA.…”

mentioning

confidence: 99%

Suppression of Photosynthetic Gene Expression in Roots Is Required for Sustained Root Growth under Phosphate Deficiency

Kang

Tian

et al. 2014

Plant Physiology

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Plants cope with inorganic phosphate (Pi) deficiencies in their environment by adjusting their developmental programs and metabolic activities. For Arabidopsis (Arabidopsis thaliana), the developmental responses include the inhibition of primary root growth and the enhanced formation of lateral roots and root hairs. Pi deficiency also inhibits photosynthesis by suppressing the expression of photosynthetic genes. Early studies showed that photosynthetic gene expression was also suppressed in Pi-deficient roots, a nonphotosynthetic organ; however, the biological relevance of this phenomenon remains unknown. In this work, we characterized an Arabidopsis mutant, hypersensitive to Pi starvation7 (hps7), that is hypersensitive to Pi deficiency; the hypersensitivity includes an increased inhibition of root growth. HPS7 encodes a tyrosylprotein sulfotransferase. Accumulation of HPS7 proteins in root tips is enhanced by Pi deficiency. Comparative RNA sequencing analyses indicated that the expression of many photosynthetic genes is activated in roots of hps7. Under Pi deficiency, the expression of photosynthetic genes in hps7 is further increased, which leads to enhanced accumulation of chlorophyll, starch, and sucrose. Pi-deficient hps7 roots also produce a high level of reactive oxygen species. Previous research showed that the overexpression of GOLDEN-like (GLK) transcription factors in transgenic Arabidopsis activates photosynthesis in roots. The GLK overexpressing (GLK OX) lines also exhibit increased inhibition of root growth under Pi deficiency. The increased inhibition of root growth in hps7 and GLK OX lines by Pi deficiency was completely reversed by growing the plants in the dark. Based on these results, we propose that suppression of photosynthetic gene expression is required for sustained root growth under Pi deficiency.

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“…PDR2 encodes an endoplasmic reticulum (ER)-localized P 5 -type ATPase (19). PDR2 and LPR1 have been proposed to orchestrate RAM exhaustion in a genetically interacting route under low-Pi conditions (19). Whereas the precise function of PDR2 has not been determined, LPR1 is essential for primary root inhibition under lowPi conditions and it has been shown to have ferroxidase activity (16,19,20).…”

mentioning

confidence: 99%

“…A mutant that is hypersensitive to low Pi, phosphate deficiency response 2 (pdr2), and triggers the root system response to low Pi availability faster that the wild type (WT) was also reported (18). PDR2 encodes an endoplasmic reticulum (ER)-localized P 5 -type ATPase (19). PDR2 and LPR1 have been proposed to orchestrate RAM exhaustion in a genetically interacting route under low-Pi conditions (19).…”

mentioning

confidence: 99%

Malate-dependent Fe accumulation is a critical checkpoint in the root developmental response to low phosphate

Mora-Macías

Ojeda-Rivera

Gutiérrez-Alanís

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

228

151

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Low phosphate (Pi) availability constrains plant development and seed production in both natural and agricultural ecosystems. When Pi is scarce, modifications of root system architecture (RSA) enhance the soil exploration ability of the plant and lead to an increase in Pi uptake. In Arabidopsis, an iron-dependent mechanism reprograms primary root growth in response to low Pi availability. This program is activated upon contact of the root tip with low-Pi media and induces premature cell differentiation and the arrest of mitotic activity in the root apical meristem, resulting in a short-root phenotype. However, the mechanisms that regulate the primary root response to Pi-limiting conditions remain largely unknown. Here we report on the isolation and characterization of two low-Pi insensitive mutants (lpi5 and lpi6), which have a long-root phenotype when grown in low-Pi media. Cellular, genomic, and transcriptomic analysis of low-Pi insensitive mutants revealed that the genes previously shown to underlie Arabidopsis Al tolerance via root malate exudation, known as SENSITIVE TO PROTON RHIZOTOXICITY (STOP1) and ALUMINUM ACTIVATED MALATE TRANSPORTER 1 (ALMT1), represent a critical checkpoint in the root developmental response to Pi starvation in Arabidopsis thaliana. Our results also show that exogenous malate can rescue the long-root phenotype of lpi5 and lpi6. Malate exudation is required for the accumulation of Fe in the apoplast of meristematic cells, triggering the differentiation of meristematic cells in response to Pi deprivation.

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ER-resident proteins PDR2 and LPR1 mediate the developmental response of root meristems to phosphate availability

Cited by 228 publications

References 42 publications

Plasticity of the Arabidopsis Root System under Nutrient Deficiencies

Plasticity of the Arabidopsis Root System under Nutrient Deficiencies

Suppression of Photosynthetic Gene Expression in Roots Is Required for Sustained Root Growth under Phosphate Deficiency

Malate-dependent Fe accumulation is a critical checkpoint in the root developmental response to low phosphate

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