ER-HOXB8 cell line, a new tool for the study of osteoclasts in osteoarthritis.

Abstract

obtain mice with Osx-Cre-directed S1P ablation (S1P Osx mice). S1P Osx mice and wild type (WT) littermates were studied by histology, in situ hybridization analysis (ISH), immunofluorescence (IF), real-time (RT) PCR and micro-computed tomography (mCT) techniques. Osteogenic differentiation assays analyzing for osteoblast differentiation in vitro were conducted with bone marrow harvested from 21 day old WT and mutant mice. Results: Mice with a homozygous deletion for S1P (S1P f/f ;Osx-Cre mice or S1P cko-Osx ) suffer from severe chondrodysplasia; mice heterozygous for S1P deletion (S1P þ/f-Osx ) have a size intermediate to S1P cko-Osx mice and WT littermates. Additionally, these mice are also kyphotic that gets more pronounced with age and was confirmed by mCT analysis. In situ