Equivalent clinical outcome after vitrified‐thawed blastocyst transfer using semi‐automated embryo vitrification system compared with manual vitrification method

Atsuko, Miwa; Noguchi, Yukiko; Hosoya, Kayo; Mori, Yasuyuki; Sato, Takuma; Kasahara, Yuta; Hidaka, Masaaki; Hayashi, Hiroshi

doi:10.1002/rmb2.12320

Cited by 13 publications

(8 citation statements)

References 21 publications

(34 reference statements)

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“…Recently, a retrospective study on human embryos compared survival rate and clinical and perinatal outcomes after vitrification and worming of single blastocysts with Gavi (398 cases) or with the Cryotop method (Kitazato Corporation; 208 cases). Gavi showed comparable clinical outcomes to the manual vitrification method resulting as an alternative vitrification procedure in IVF (103).…”

Section: Vitrificationmentioning

confidence: 87%

Are we approaching automated assisted reproductive technology? Embryo culture, metabolomics, and cryopreservation

Casciani¹,

Galliano

Franasiak

et al. 2021

F&S Reviews

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For decades, assisted reproductive technology (ART) procedures have been performed manually thanks to the meticulous work of skilled embryologists. Recently, new technologies have been developed with three main scopes: improving embryo culture conditions; making diagnostic evaluations more consistent and reliable; and allowing ART procedures to become progressively less subjective and operatordependent. This review aimed to answer the following questions: is automation likely to be successfully incorporated into the in vitro fertilization laboratory and clinical ART in the future? If so, would such automation result in improved outcomes in ART?An electronic search of PubMed was performed to identify articles in English language that addressed automation in ART. Studies were classified in decreasing categories: randomized controlled trials; prospective controlled trials; prospective noncontrolled trials; retrospective studies; and experimental studies. Research and development data from investigators were included.There are a number of separate platforms that have been developed until now to address different parts of the ART process: gradual change of embryo culture medium; noninvasive embryo monitoring with time-lapse or metabolome analysis; and automated vitrification. It is conceivable that future automation enhancements in the in vitro fertilization laboratory may improve consistency and throughput and reduce the risk of human error associated with the performance of repetitive tasks. Nevertheless, a need remains for a platform able to integrate all separate technologies, capable of successfully interconnecting them in a way that assures continued chain of custody for the gametes and embryos. Moreover, controlled trials will be fundamental to demonstrate the usefulness of automation in ART. (Fertil Steril Rev Ò 2021;2:251-64. Ó2021 by American Society for Reproductive Medicine.

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Section: Vitrificationmentioning

confidence: 87%

Are we approaching automated assisted reproductive technology? Embryo culture, metabolomics, and cryopreservation

Casciani¹,

Galliano

Franasiak

et al. 2021

F&S Reviews

View full text Add to dashboard Cite

show abstract

“…The first automated cryopreservation (vitrification) device, Gavi® (Genea Biomedx, Australia), showed equivalent clinical efficiency in embryo survival post-embryo thaw when compared to existing manual methods [86]. The automated equilibration of embryos prior to vitrification offers reduced labour intensity and improved accuracy with the maintenance of optimal conditions such as embryo positioning, cryoprotectant concentration, exposure time, cooling rates, volume, and temperature [87].…”

Section: Cryopreservation and Cryostoragementioning

confidence: 99%

Automation in ART: Paving the Way for the Future of Infertility Treatment

et al. 2022

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In vitro fertilisation (IVF) is estimated to account for the birth of more than nine million babies worldwide, perhaps making it one of the most intriguing as well as commoditised and industrialised modern medical interventions. Nevertheless, most IVF procedures are currently limited by accessibility, affordability and most importantly multistep, labour-intensive, technically challenging processes undertaken by skilled professionals. Therefore, in order to sustain the exponential demand for IVF on one hand, and streamline existing processes on the other, innovation is essential. This may not only effectively manage clinical time but also reduce cost, thereby increasing accessibility, affordability and efficiency. Recent years have seen a diverse range of technologies, some integrated with artificial intelligence, throughout the IVF pathway, which promise personalisation and, at least, partial automation in the not-so-distant future. This review aims to summarise the rapidly evolving state of these innovations in automation, with or without the integration of artificial intelligence, encompassing the patient treatment pathway, gamete/embryo selection, endometrial evaluation and cryopreservation of gametes/embryos. Additionally, it shall highlight the resulting prospective change in the role of IVF professionals and challenges of implementation of some of these technologies, thereby aiming to motivate continued research in this field. Graphical abstract

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“…At the same time, making each act of vitrification is traceable. Assessment of the efficiency of the semi-automated platform as compared with the reference method (manual) was recently made at the zygote [ 22 ], cleaved embryo [ 20 ] or blastocyst stages [ 29 ]. However, there is no comparative study on the expected oocyte survival rate using the Gavi® system.…”

Section: Introductionmentioning

confidence: 99%

Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses

et al. 2022

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Background Indications of oocyte vitrification increased substantially over the last decades for clinical and ethical reasons. A semi-automated vitrification system was recently developed making each act of vitrification reproducible. In this study, we evaluated the efficiency of the semi-automated technique of oocyte vitrification by survival rate, morphometric assessment and resistance to empty micro-injection gesture as compared with a manual method. Additionally, we intended to evaluate transcriptomic consequences of both techniques using single-cell RNA-seq technology. Results Post-warming survival rate, oocyte surfaces and resistance to empty micro-injection were comparable between semi-automated and manual vitrification groups. Both oocyte vitrification techniques showed limited differences in the resulting transcriptomic profile of sibling oocytes since only 5 differentially expressed genes were identified. Additionally, there was no difference in median transcript integrity number or percentage of mitochondrial DNA between the two groups. However, a total of 108 genes were differentially expressed between fresh and vitrified oocytes (FDR < 0.05) and showed over-represented of genes related to important cellular process. Conclusions Our results provide reassurance about the influence of semi-automation as compared with the manual vitrification method. Concerning oocyte vitrification itself, no tight common transcriptomic signature associated has been observed across studies. Trial registration NCT03570073.

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Equivalent clinical outcome after vitrified‐thawed blastocyst transfer using semi‐automated embryo vitrification system compared with manual vitrification method

Cited by 13 publications

References 21 publications

Are we approaching automated assisted reproductive technology? Embryo culture, metabolomics, and cryopreservation

Are we approaching automated assisted reproductive technology? Embryo culture, metabolomics, and cryopreservation

Automation in ART: Paving the Way for the Future of Infertility Treatment

Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses

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