Equivalence of Laser Scanning Cytometric and Flow Cytometric Immunophenotyping of Lymphoid Lesions in Cytologic Samples

Abstract

The immunophenotype of lymphoid cytologic samples obtained by laser scanning cytometry (LSC) and flow cytometry (FC) was compared in 72 cases composed of a series of 23 cases with simultaneous LSC and FC immunophenotyping and a second series of 49 cases in which nonsimultaneous immunophenotyping was performed. In both series, no discordance in the population immunophenotype was found that would result in changes in diagnostic classification, although minor discordance in some antigens was found, predominantly affecting FMC7, CD11c, and CD23. The immunophenotype obtained by LSC shows a high degree of concordance with that obtained by FC and generates results that are diagnostically equivalent. Potential explanations for the discordant markers include differences related to the techniques, differences in the fluorochrome-labeled antibodies, technical factors, differences in antigen expression related to anatomic sites, temporal variations, and interpretive variances.

The role of fine-needle aspiration in the diagnosis of thyroid lymphoma: a retrospective study of nine cases and review of published series

“…Needles were rinsed in saline, and the resulting specimen was used for immunophenotyping by immunocytochemistry (ICC) or laser scanning cytometry (LSC) following the Clatch protocol 20 21. LSC is an alternative method of cytometry that has been demonstrated to give equivalent surface immunophenotyping results to FC 22. Cytospin slides and cell block preparations were used for immunohistochemistry.…”

Cytomorphologic findings of B‐cell lymphomas with concurrent IGH/BCL2 and MYC rearrangements (dual‐translocation lymphomas)

“…LSC has been validated in our laboratory and has produced results equivalent to those produced by flow cytometry. 12 The antibody clones and concentrations, fluorochromes, and suppliers have been described previously.…”

Maximizing the yield of lymph node cytology

“…The antibody clones and concentrations, fluorochromes, and suppliers have been previously detailed. 4 A limited panel tailored to the morphologic differential diagnosis is performed in cases with low cellularity. Interpretation of immunophenotyping data is performed by the cytopathologists and integrated with the cytomorphological findings in the final cytologic report.…”