The ( 13 C) isotope separation column is a complex process with many inputs and outputs. Proper literature is scarce (or inexistent) due to the fact that only a few plants exist in the world and due to data protection issues. In order to design an advanced control strategy, implemented on a process computer in LabVIEW™, in the present work was developed a mathematical model, described by a transfer function matrix using identification methods. The validation of the model is accomplished by the comparison of plant data and simulation results using Matlab™ and LabVIEW™.