Equipment and preliminary results for orthogonal pressurized planar electrochromatography

Some theoretical considerations on preparative separation with orthogonal pressurised planar electrochromatography

“…In our previous papers, we had presented the principle of action, construction of equipment and preliminary results from the continuous separation of substances with different electrophoretic mobility using OPPEC technique [14][15][16]. Moreover, the last report suggests that our technique is also suitable for preparative separation of substances having the same electrophoretic mobility if these substances differ regarding retention [17].…”

“…1 -mobile phase in; 2 -electrodes; 3 -adsorbent layer; 4 -periodic feeding of the separation system with mixture solution through inlet capillary; 5 -entry mixture position; 6-8 -solute bands; 9, 11 -outlet capillaries; 10, 13-14 -reservoirs of a fraction collector; 12 -two-way valve the direction of mobile phase flow. The separated components leave the separation system by the outlet capillaries (9,11), and they are collected in the reservoirs of a fraction collector (10), (13) and (14). In addition, the solute band labelled as (7) is formed by two components showing the same electrophoretic mobility.…”

“…The solute bands labelled as (6) and (8) in contrast to the solute band labelled as (7), are presented as dashed lines because these solute bands are formed by single substances. The solution of two target components separated in the band (7) leaves the separation system by one outlet capillary (11), from among three others (9), into the reservoirs labelled as (13) and (14). As the target components of the mixture are separated, they are collected by the two-way valve (12) that is switched according to their periodic outflow from the separation system.…”

“…Finally, a continuous separation can also be performed by using orthogonal pressurised planar electrochromatography (OPPEC) . However, it should be emphasised that such a process so that, as with the FFE has a certain constraint when components have the same electrophoretic mobility.…”

TLC-Direct Bioautography as a High Throughput Method for Detection of Antimicrobials in Plants

“…A new separation technique, so-called orthogonal pressurized planar electrochromatography (OPPEC), introduced by T. Dzido and co-workers, seems to be a promising solution. This combination of OPLC and pressurized planar electrochromatography (PPEC) enables on-line application and continuous, micropreparative, orthogonal sample separation [59].…”