Equine odontoclastic tooth resorption and hypercementosis (EOTRH): microspatial distribution of trace elements in hypercementosis-affected and unaffected hard dental tissues

Wright, Alexandra L; Earley, Edward T.; Austin, Christine; Arora, Manish

doi:10.1038/s41598-023-32016-6

Cited by 2 publications

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“…Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) is a well-established technique used to map elements in teeth, as well as other biological tissues, at a micrometer spatial resolution ( 28 – 30 ). Thus, the distribution of trace elements along histologic layers in teeth has been applied mainly to rodent, human, primate, and most recently equine teeth ( 16 , 31 – 35 ). This study aimed to establish the feasibility of the use of LA-ICP-MS to measure trace element levels in feline hard dental tissues (enamel, dentin, and cementum) and to characterize the microspatial distribution of tooth-seeking trace elements (lead, strontium, barium, and zinc) in feline teeth.…”

Section: Introductionmentioning

confidence: 99%

Microspatial distribution of trace elements in feline dental hard tissues: early life exposure to essential and toxic elements

et al. 2023

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IntroductionTrace elements play a key role in dental tissue development, as dental hard tissues accumulate both essential and toxic trace elements during mineralization. Characterization of the spatial accumulation pattern of trace elements may provide insight into exposure to toxic elements over time and to the nature of disease processes affecting the hard dental tissues. Here, we present the first report of the use of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) to map the microspatial distribution of multiple trace elements, essential and toxic, across feline dental hard tissues.MethodsEleven teeth were extracted from 8 cats. Nine teeth were from 7 cats diagnosed with idiopathic tooth resorption on intraoral radiographs prior to extraction. Two teeth were included from a cadaver that had no signs of tooth resorption on intraoral radiographs. The normal dental tissue was analyzed from each sample using LA-ICP-MS to map the microspatial distribution of essential and toxic trace elements across feline enamel, dentin, and cementum.ResultsResults showed a higher accumulation of barium and strontium in coronal dentin as compared to root dentin. The timing of the accumulation mirrors nursing timelines seen in teeth from human and non-human primates, consistent with barium and strontium being sourced from maternal milk. Results also showed a higher uptake of lead in the coronal dentin, suggesting this lead exposure was likely passed from mother to offspring.DiscussionThis work characterizes a baseline for elemental distribution in feline teeth linked to early life exposure to toxic elements such as lead and provides a framework for future studies investigating long-term environmental exposures to trace elements, essential and toxic, and their involvement in feline systemic and dental diseases.

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