Equine gammaherpesvirus 2 (EHV2) is latent in B lymphocytes

Drummer, Heidi E.; Reubel, G. H.; Studdert, M. J.

doi:10.1007/bf01718313

Cited by 57 publications

(45 citation statements)

References 25 publications

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“…The PCR analysis of the collected PBL specimens revealed that as many as 56 to 68% of the horses in the two Swedish stables and 71% of the tested English horses carried EHV-2 DNA in the PBLs. Thus our results strongly support the previous observation that EHV-2 is latent in B lymphocytes [10].…”

Section: Discussionsupporting

confidence: 93%

Prevalence of equine herpesvirus types 2 and 5 in horse populations by using type-specific PCR assays

Nordengrahn¹,

Mérza²,

Ros

et al. 2002

Vet. Res.

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-Equine herpesvirus types 2 and 5 (EHV-2 and EHV-5) have a rather unclear pathogenicity and distribution within the equid population. In order to gain more information on the prevalence of these two viruses, type-specific PCR assays were developed to detect viral DNA in nasal specimens and in peripheral blood leukocytes (PBLs) of adult horses and foals from various regions of Europe, i.e. Sweden, Hungary and the United Kingdom. In adult horses, the prevalence of EHV-2 in PBLs was up to 68% in Sweden and 71% in the United Kingdom. EHV-2 DNA was detected in the PBLs from all the foals tested in all countries and most (93%) of the nasal specimens also yielded positive results. The prevalence of EHV-5 DNA in the PBLs of foals in Hungary was 15 and 24% in adult horses in the United Kingdom. This observation was among the very few reports of the presence of EHV-5 in horses. In summary, the specific PCR assays revealed important data on the occurrence and distribution of EHV-2 and EHV-5 in large horse populations. The findings indicated that infection with EHV-5 occurred later than EHV-2 in foals. This study may contribute to a better understanding of the etiological role of these gammaherpesviruses in equine diseases. Chez les chevaux adultes, la prévalence de EHV-2 dans les LSP atteignait 68 % en Suède et 71 % au Royaume-Uni. L'ADN de EHV-2 a été détecté dans les LSP de tous les poulains testés, et la plupart (93 %) des prélèvements nasaux étaient également positifs. La préva-lence de l'ADN de EHV-5 dans les LSP des poulains en Hongrie était de 15 % et de 24 % chez les chevaux adultes au Royaume-Uni. Cette observation fait partie des très rares signalements de la présence de EHV-5 chez les chevaux. En résumé, les tests PCR spécifiques ont révélé des données importantes sur la présence et la distribution de EHV-2 et EHV-5 dans d'importantes populations de chevaux. Les résultats ont montré que l'infection par le EHV-5 se produisait plus tard que celle par le EHV-2 chez le poulain. Cette étude apporte une meilleure compréhension du rôle étiologique de ces herpesvirus gamma dans les maladies équines.infections équines / Gammaherpesvirinae / EHV-2 / EHV-5 / PCR

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Section: Discussionsupporting

confidence: 93%

Prevalence of equine herpesvirus types 2 and 5 in horse populations by using type-specific PCR assays

Nordengrahn¹,

Mérza²,

Ros

et al. 2002

Vet. Res.

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“…4 Equine gammaherpesvirus-5 has also been implicated in the pathogenesis of a distinctive syndrome of pulmonary fibrosis of adult horses (Williams KJ, Jackson CA, Scott MA, et al 14 The equine herpesviruses are likely spread by respiratory infection, and the viruses are periodically excreted in the nasal secretions of infected horses. Equine gammaherpesvirus-2 latently infects B lymphocytes 5 and can be isolated from the peripheral blood mononuclear cells (PBMC) of approximately 90% of apparently healthy horses. 12,15 Coinfection of individual horses with EHV-2 and EHV-5 is common, although EHV-5 infection is less prevalent than EHV-2.…”

mentioning

confidence: 99%

Isolation of Equine Herpesvirus-5 from Blood Mononuclear Cells of a Gelding

et al. 2006

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Abstract. Horses are commonly infected by herpesviruses, but isolation of equine herpesvirus-5 (EHV-5) has only infrequently been reported. We describe the isolation and characterization of a strain of EHV-5 from the blood mononuclear cells of a healthy adult horse in California. The virus was initially identified by EHV-5 specific polymerase chain reaction (PCR), and it caused lytic infection of cultured rabbit kidney cells only after repeated serial passage. Virions with characteristic herpesvirus morphology were readily demonstrated in cell culture lysate by transmission electron microscopy. A portion of the glycoprotein B gene of this strain of EHV-5 had 99% identity to the published EHV-5 sequence, and it was clearly distinguishable from other EHV (1-4) by virus-specific PCR assays. Prevalence of EHV-5 infection in a group of young racehorses was estimated at 64% using the EHV-5 specific PCR on nasopharyngeal secretions.

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“…3 As with other gammaherpesviruses, latency in lymphocytes has been demonstrated for EHV2. 9 A second gammaherpesvirus of horses, EHV5, has been isolated but is less well characterized when compared with EHV2 and has not yet been associated with disease, 1,6,40 although the results of experimental inoculations of EHV5 have not been reported. Related but distinct gammaherpesviruses have recently been identified by consensus primer PCR in zebras and a Somali wild ass.…”

mentioning

confidence: 99%