Equine Fecal Microbiota Changes Associated With Anthelmintic Administration

Kunz, I.G.Z.; Reed, Kailee J.; Metcalf, Jessica L.; Hassel, Diana M.; Coleman, Robert J.; Hess, Tanja; Coleman, S. J.

doi:10.1016/j.jevs.2019.01.018

Cited by 33 publications

(46 citation statements)

References 165 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, the authors cannot rule out such an effect if the FPP was given at higher doses or for a longer period of time. Our results concur with those reported in other species, where fenbendazole did not have an effect on the hindgut microbiota of horses [20] or the gut microbiome of mice [21] and a moxidectin praziquantel combination product did not have a major effect on the fecal microbiota of horses [22]. In healthy staff-owned dogs administered fenbendazole, there was a significant effect of treatment group on the fecal abundance of Fusobacterium and E. coli.…”

Section: Bacterial Groupsupporting

confidence: 91%

“…However, most studies in other species did not find major effects of various anthelmintic agents on the intestinal microbiome. Specifically, fenbendazole did not have an effect on the hindgut microbiota of horses [20] or the gut microbiome of mice [21] and a moxidectin praziquantel combination product did not have a major effect on the fecal microbiota of horses [22]. Differences between the results of these studies may be attributable to species differences, different active ingredients studied or dosages and the different definitions of what constitutes an important change in the gastrointestinal microbiome.…”

Section: Discussionmentioning

confidence: 99%

“…To the authors' knowledge, the effect of administration of an anthelmintic agent (combination product FPP or fenbendazole) on the fecal microbiome of dogs has not yet been described. However, fenbendazole has not been shown to have a major effect on the hindgut microbiota of horses [20] or the gut microbiome of mice [21] and a moxidectin praziquantel combination product did not have a major effect on the fecal microbiota of horses [22].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of the effects of anthelmintic administration on the fecal microbiome of healthy dogs with and without subclinical Giardia spp. and Cryptosporidium canis infections

et al. 2020

View full text Add to dashboard Cite

Background The gastrointestinal microbiome plays an important role in host health and there is increasing concern regarding the deleterious effects of pharmaceuticals on the fecal microbiome. The effect of anthelmintic therapy on the fecal microbiome in dogs has not yet been evaluated. The purpose of this study was to evaluate the effect of anthelmintic administration on the fecal microbiome of dogs with and without subclinical Giardia species and Cryptosporidium canis infections. Methodology/Principal findings Part 1: 6 healthy adult research beagles with subclinical giardiasis and cryptosporidiosis were administered a commercially available preparation of febantel combined with pyrantel and praziquantel (FPP) orally daily for three days. Part 2: 19 healthy staff-owned dogs without giardiasis or cryptosporidiosis were divided into a treatment group (n = 9) that was administered fenbendazole orally daily for five days and an untreated control group (n = 10). For both parts of the study, feces were collected at multiple time points before and after anthelmintic (FPP or fenbendazole) administration. Fecal DNA was extracted for Illumina sequencing of the bacterial 16S rRNA gene and qPCR assays. Neither FPP nor fenbendazole treatment caused a significant change in alpha or beta diversity or the relative abundance of bacterial species. Upon univariate statistical analysis neither FPP or fenbendazole caused minimal changes in the fecal microbiota. Conclusion FPP administration was associated with minimal alterations of the fecal microbiome of healthy research beagles with subclinical giardiasis and cryptosporidiosis. Fenbendazole

show abstract

Section: Bacterial Groupsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of the effects of anthelmintic administration on the fecal microbiome of healthy dogs with and without subclinical Giardia spp. and Cryptosporidium canis infections

et al. 2020

View full text Add to dashboard Cite

show abstract

“…4 The correlation coefficient plot from the OPLS-DA model built with the metabolic profiles of samples from the fermentations of faeces collected 16 h after moxidectin administration (R 2 = 0.69, Q 2 Y = 0.48) taxonomic changes in our study. The sampling time points used by Kunz et al [22] differed to those in our study, our changes in faecal microbiota were seen between 40 and 160 h after moxidectin treatment. This exceeded the time points measured by Kunz et al [22] which only extended to 2 days post dosing, this is one possible explanation for the differences in results between studies.…”

Section: Discussioncontrasting

confidence: 65%

“…appears also to be influenced by time as a similar pattern is seen in the control group but at lower relative abundance. Kunz et al [22] observed no changes at all in faecal microbiota profile following moxidectin and praziquantel administration, in contrast we observed some moderate Fig. 3 The pH of the fermentation inoculum of faeces from the horses in the moxidectin and control groups over all sampling points (0-160 h).…”

Section: Discussionmentioning

confidence: 47%

Bugs and drugs: a systems biology approach to characterising the effect of moxidectin on the horse’s faecal microbiome

2020

View full text Add to dashboard Cite

Background Anthelmintic treatment is a risk factor for intestinal disease in the horse, known as colic. However the mechanisms involved in the onset of disease post anthelmintic treatment are unknown. The interaction between anthelmintic drugs and the gut microbiota may be associated with this observed increase in risk of colic. Little is known about the interaction between gut microbiota and anthelmintics and how treatment may alter microbiome function. The objectives of this study were: To characterise (1) faecal microbiota, (2) feed fermentation kinetics in vitro and (3) metabolic profiles following moxidectin administration to horses with very low (0 epg) adult strongyle burdens. Hypothesis: Moxidectin will not alter (1) faecal microbiota, (2) feed fermentation in vitro, or, (3) host metabolome. Results Moxidectin increased the relative abundance of Deferribacter spp. and Spirochaetes spp. observed after 160 h in moxidectin treated horses. Reduced in vitro fibre fermentation was observed 16 h following moxidectin administration in vivo (P = 0.001), along with lower pH in the in vitro fermentations from the moxidectin treated group. Metabolic profiles from urine samples did not differ between the treatment groups. However metabolic profiles from in vitro fermentations differed between moxidectin and control groups 16 h after treatment (R2 = 0.69, Q2Y = 0.48), and within the moxidectin group between 16 h and 160 h post moxidectin treatment (R2 = 0.79, Q2Y = 0.77). Metabolic profiles from in vitro fermentations and fermentation kinetics both indicated altered carbohydrate metabolism following in vivo treatment with moxidectin. Conclusions These data suggest that in horses with low parasite burdens moxidectin had a small but measurable effect on both the community structure and the function of the gut microbiome.

show abstract

A high-throughput DNA sequencing study of fecal bacteria of seven Mexican horse breeds

Hernández-Quiroz

Murugesan²,

Flores-Rivas³

et al. 2022

Arch Microbiol

View full text Add to dashboard Cite

Equine Fecal Microbiota Changes Associated With Anthelmintic Administration

Cited by 33 publications

References 165 publications

Evaluation of the effects of anthelmintic administration on the fecal microbiome of healthy dogs with and without subclinical Giardia spp. and Cryptosporidium canis infections

Evaluation of the effects of anthelmintic administration on the fecal microbiome of healthy dogs with and without subclinical Giardia spp. and Cryptosporidium canis infections

Bugs and drugs: a systems biology approach to characterising the effect of moxidectin on the horse’s faecal microbiome

A high-throughput DNA sequencing study of fecal bacteria of seven Mexican horse breeds

Contact Info

Product

Resources

About