Equine endometrial vascular pattern changes during the estrous cycle examined by Narrow Band Imaging hysteroscopy

Otzen, Henning; Sieme, Harald; Oldenhof, Harriëtte; Kassens, Ana; Ertmer, Franziska; Rode, Kristina; Müller, Kristin; Klose, Kristin; Rohn, Karl; Schoon, Heinz-Adolf; Meinecke, B.

doi:10.1016/j.anireprosci.2016.01.006

Cited by 9 publications

(4 citation statements)

References 30 publications

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“…Galvão et al ( 42 , 66 ) showed that cytokines interact with nitic oxide synthases and influence luteal angiogenesis in mares as angiogenic factors themselves can also modulate luteal secretory function. Previously, Otzen et al ( 67 ) found that PGE 2 stimulates vascular endothelial growth factor (VEGF), which participates in the regeneration and expansion of the equine uterine blood vessel network. Moreover, VEGF has been reported to effectively modulate luteal secretory function of equine CL (P 4 and PGE 2 production) ( 66 ).…”

Section: Discussionmentioning

confidence: 99%

The Effects of Prostaglandin E2 Treatment on the Secretory Function of Mare Corpus Luteum Depends on the Site of Application: An in vivo Study

et al. 2022

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We examined the effect of prostaglandin (PG) E2 on the secretory function of equine corpus luteum (CL), according to the application site: intra-CL injection vs. an intrauterine (intra-U) administration. Moreover, the effect of intra-CL injection vs. intra-U administration of both luteotropic factors: PGE2 and human chorionic gonadotropin (hCG) as a positive control, on CL function was additionally compared. Mares were assigned to the groups (n = 6 per group): (1) an intra-CL saline injection (control); (2) an intra-CL injection of PGE2 (5 mg/ml); (3) an intra-CL injection of hCG (1,500 IU/ml); (4) an intra-U saline administration (control); (5) an intra-U administration of PGE2 (5 mg/5 ml); (6) an intra-U administration of hCG (1,500 IU/5 ml). Progesterone (P4) and PGE2 concentrations were measured in blood plasma samples collected at −2, −1, and 0 (pre-treatment), and at 1, 2, 3, 4, 6, 8, 10, 12, and 24 h after treatments. Moreover, effects of different doses of PGE2 application on the concentration of total PGF2α (PGF2α and its main metabolite 13,14-dihydro-15-keto-prostaglandin F2α– PGFM) was determined. The time point of PGE2, hCG, or saline administration was defined as hour “0” of the experiment. An intra-CL injection of PGE2 increased P4 and PGE2 concentrations between 3 and 4 h or at 3 and 12 h, respectively (p < 0.05). While intra-U administration of PGE2 elevated P4 concentrations between 8 and 24 h, PGE2 was upregulated at 1 h and between 3 and 4 h (p < 0.05). An intra-CL injection of hCG increased P4 concentrations at 1, 6, and 12 h (p < 0.05), while its intra-U administration enhanced P4 and PGE2 concentrations between 1 and 12 h or at 3 h and between 6 and 10 h, respectively (p < 0.05). An application of PGE2, dependently on the dose, supports equine CL function, regardless of the application site, consequently leading to differences in both P4 and PGE2 concentrations in blood plasma.

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Section: Discussionmentioning

confidence: 99%

The Effects of Prostaglandin E2 Treatment on the Secretory Function of Mare Corpus Luteum Depends on the Site of Application: An in vivo Study

et al. 2022

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“…Human gynecological studies have been used to evaluate cervical lesions, endometriosis, and endometrial lesions, serving as "optical biopsies" with criteria currently in development (Kisu et al 2012). Equine studies have shown that blood vessel numbers in the endometrium are higher in diestrus than estrus without changes in vessel diameter or differences within the uterus and differences in vascular densities in mares with improved Kenney Doig endometrial biopsy scores compared to poorer scoring mares (Otzen, Sieme, Oldenhof, Kassens, et al 2016;. The use of hysteroscopy in real time evaluation allows for the comparison of the endometrial vasculature throughout the estrus cycle as well as when compared to various hormone profiles, providing information about the functional uterine anatomy including blood and subsequently nutritional flow throughout the endometrium in healthy and degenerate tissues.…”

Section: Diagnostic and Therapeutic Uses Of Hysteroscopymentioning

confidence: 99%

The Use of Hysteroscopy to Identify and Treat Reproductive Tract Abnormalities in the Mare

Eaton

2024

Preprint

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Hysteroscopy is a complementary tool to the traditional transrectal palpation and ultrasonography commonly performed by equine practitioners. Once mastered, it is a quick and useful diagnostic tool for the general and targeted evaluation of the endometrium and provides real time information about potential causes of subfertility. Samples of intrauterine fluid and the endometrium can be taken via transendoscopic catheters or infusion pipettes or via the use of traditional endometrial biopsy forceps. In addition to being a useful diagnostic tool, treatments can often be performed at the same time the pathologies are identified, providing mare owners with information and resolution of problems. With the development of additional technologies, hysteroscopy allows for optical biopsies to be performed, which will likely increase in use as the software moves from the research laboratories to clinical practice. Hysteroscopy is an easily learned, useful clinical skill for the identification of abnormalities in the mares’ reproductive tract.

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“…Vascular density in histological sections of the equine endometrium was found to be similar in the follicular and luteal phase (da Costa et al., 2007; Ferreira‐Dias & Mateus, 2003; Ferreira‐Dias, Serrao, Durao, & Silva, 2001; Otzen et al., 2016), indicating that vascular and stromal growth are coordinated. Similar results were presented by Merkl et al.…”

Section: Angiogenesis Research In Equine Reproductionmentioning

confidence: 99%

“…Using narrow band imaging hysteroscopy, Otzen et al. (2016) found an increase in vascular density from oestrus towards diestrus, while results from histomorphology and immunohistochemistry suggested that the higher vascularity measured in vivo is not due to angiogenesis, but caused by a higher perfusion of the existing capillaries (Otzen et al., 2016).…”

Section: Angiogenesis Research In Equine Reproductionmentioning

confidence: 99%

Endothelial cells and angiogenesis in the horse in health and disease—A review

Rieger

Kaessmeyer

Masri

et al. 2020

Anat Histol Embryol

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The cardiovascular system is the first functional organ in the embryo, and its blood vessels form a widespread conductive network within the organism. Blood vessels develop de novo, by the differentiation of endothelial progenitor cells (vasculogenesis) or by angiogenesis, which is the formation of new blood vessels from existing ones. This review presents an overview of the current knowledge on physiological and pathological angiogenesis in the horse including studies on equine endothelial cells. Principal study fields in equine angiogenesis research were identified: equine endothelial progenitor cells; equine endothelial cells and angiogenesis (heterogeneity, markers and assessment); endothelial regulatory molecules in equine angiogenesis; angiogenesis research in equine reproduction (ovary, uterus, placenta and conceptus, testis); angiogenesis research in pathological conditions (tumours, ocular pathologies, equine wound healing, musculoskeletal system and laminitis). The review also includes a table that summarizes in vitro studies on equine endothelial cells, either describing the isolation procedure or using previously isolated endothelial cells. A particular challenge of the review was that results published are fragmentary and sometimes even contradictory, raising more questions than they answer. In conclusion, angiogenesis is a major factor in several diseases frequently occurring in horses, but relatively few studies focus on angiogenesis in the horse. The challenge for the future is therefore to continue exploring new therapeutic angiogenesis strategies for horses to fill in the missing pieces of the puzzle.

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Equine endometrial vascular pattern changes during the estrous cycle examined by Narrow Band Imaging hysteroscopy

Cited by 9 publications

References 30 publications

The Effects of Prostaglandin E2 Treatment on the Secretory Function of Mare Corpus Luteum Depends on the Site of Application: An in vivo Study

The Effects of Prostaglandin E2 Treatment on the Secretory Function of Mare Corpus Luteum Depends on the Site of Application: An in vivo Study

The Use of Hysteroscopy to Identify and Treat Reproductive Tract Abnormalities in the Mare

Endothelial cells and angiogenesis in the horse in health and disease—A review

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