Equine CRISP-3: primary structure and expression in the male genital tract

Schambony, Alexandra; Gentzel, Marc; Wolfes, Heiner; Raida, Manfred; Neumann, Ulrich; Töpfer‐Petersen, Edda

doi:10.1016/s0167-4838(98)00122-8

Cited by 68 publications

(50 citation statements)

References 40 publications

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“…In several previous studies, many genes and proteins were analysed for their association with fertility in the stallion (Schambony et al 1998;Hamann et al 2007;Zaabal and Ahmed 2010). For example, equine cysteine-rich secretory protein (CRISP) genes were found to be significantly associated with male fertility (Schambony et al 1998).…”

Section: Discussionmentioning

confidence: 99%

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Implication of transcriptome profiling of spermatozoa for stallion fertility

Suliman

Becker

Wimmers

2018

Reprod. Fertil. Dev.

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Abstract. Poor fertility of breeding stallions is a recognised problem in the equine industry. The aim of the present study was to detect molecular pathways using two groups of stallions that differed in pregnancy rates as well as in the proportion of normal and motile spermatozoa. RNA was isolated from spermatozoa of each stallion and microarray data were analysed to obtain a list of genes for which transcript abundance differed between the groups (P #0.05, fold change $1.2). In all, there were 437 differentially expressed (DE) genes between the two groups (P # 0.05, fold change $1.2). Next, the DE genes were analysed using Database for Annotation, Visualisation, and Integrated Discovery (DAVID). Finally, ingenuity pathways analysis (IPA) was used to identify top biological functions and significant canonical pathways associated with the DE genes. Analysis using the DAVID database showed significant enrichment in the gene ontology (GO) term 'RNA binding' (P ¼ 0.05) and in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway cytokinecytokine receptor interaction (P ¼ 0.02). Furthermore, IPA analysis showed interconnected biological functions and canonical pathways involved in the regulation of spermatogenesis and male fertility. In addition, significantly enriched metabolic pathways were identified. In conclusion, the present study has identified, for the first time, molecular processes in stallion spermatozoa that could be associated with stallion fertility.

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Section: Discussionmentioning

confidence: 99%

“…For example, equine cysteine-rich secretory protein (CRISP) genes were found to be significantly associated with male fertility (Schambony et al 1998). The CRISP proteins represent the major equine fraction of seminal plasma proteins and were found to be involved in sperm-oocyte fusion (Töpfer-Petersen et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Implication of transcriptome profiling of spermatozoa for stallion fertility

Suliman

Becker

Wimmers

2018

Reprod. Fertil. Dev.

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“…The function of the glandular portion of the end part of the deferent duct has not yet been fully elucidated. Its fluid adds species-specific proteins and hormone to seminal fluid, known to associate with the sperm surface and participate in the complex events of sperm-egg interaction (Schambony et al 1998). Glutathione peroxidase and superoxide dismutase enzymes, as well as antioxidant substances and secretory proteins rich in cysteine, were found in the secretion of the glandular portion of the end part of the deferent duct by Barber & Ball (2005).…”

Section: Discussionmentioning

confidence: 99%

Genital morphology of the male South American fur seal (Arctocephalus australis) and biological implications

et al. 2012

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Male capacity for spreading genes to a great number of descendents and to determine population dynamics depend directly on the genital organs. Morphological studies in pinnipeds are scarce and the functional meaning of some characteristics has never been discussed. We hypothesized that Arctocephalus australis (A. australis) shows morphophysiological adaptations in order to guarantee the perpetuation of the species in the unique annual mating season. Seven males, dead from natural causes, had their genital organs collected and fixed for morphological description. Some features differ from other described mammalian males and are closely related to the biology and reproductive cycle of this species, as the scrotal epidermis, absence of glandular portion in the ductus deferens and spermatogenic epithelium suggest a recrudescent testis period. The corona glandis exhibits a singular arrangement: its erectile border looks like a formation of petals and its association with the os penis gives a "lily-flower" form to this region. We propose the name margo petaliformis to this particular erectile border of the corona glandis because of its similarity to a flower corola. The male genital organs of A. australis show morphological features compatible with adaptation to environment requirements and reproductive efficiency.

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“…58 CRISP3 is produced by the mouse prostatic epithelium and is present in secretions within the lumen (Figure 2). 4 Similarly, CRISP3 is produced in horse seminal vesicles 59 and is present in both horse and human seminal plasma at concentrations of 0.3-1.3 mg ml 21 and 14.8 mg ml 21 , respectively. 58,60 Despite its presence in such high concentrations, the function of these CRISPs within the ejaculate remains virtually unknown.…”

Section: Crisps At the Ejaculate And In The Accessory Sex Organsmentioning

confidence: 99%

The role of cysteine-rich secretory proteins in male fertility

Koppers¹,

Reddy²,

O'Bryan³

2010

Asian J Androl

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The cysteine-rich secretory proteins (CRISPs) are a subgroup of the CRISP, antigen 5 and Pr-1 (CAP) protein superfamily, and are found only in vertebrates. They show a strong expression bias to the mammalian male reproductive tract and the venom of poisonous reptiles. Within the male reproductive tract CRISPs have been implicated in many aspects of male germ cell biology spanning haploid germ cell development, epididymal maturation, capacitation, motility and the actual processes of fertilization. At a structural level, CRISPs are composed of two domains, a CAP domain, which has been implicated in cell-cell adhesion, and a CRISP domain, which has been shown to regulate several classes of ion channels across multiple species. Herein, we will review the current literature on the role of CRISPs in male fertility, and by inference to related non-mammalian protein, infer potential biochemical functions.

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Equine CRISP-3: primary structure and expression in the male genital tract

Cited by 68 publications

References 40 publications

Implication of transcriptome profiling of spermatozoa for stallion fertility

Implication of transcriptome profiling of spermatozoa for stallion fertility

Genital morphology of the male South American fur seal (Arctocephalus australis) and biological implications

The role of cysteine-rich secretory proteins in male fertility

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