Equilibrium Sedimentation of Macromolecules in Density Gradients

Meselson, Matthew; Stahl, Franklin W.; Vinograd, Jerome

doi:10.1073/pnas.43.7.581

Cited by 696 publications

(155 citation statements)

References 3 publications

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“…1) suggested the possible association of the low-molecular-weight polysaccharide and protein, although for preparation A it was apparent that this may not apply to all the protein present. Density-gradient ultracentrifugation (Meselson et al 1957) was used to examine the possible association of polysaccharide and protein. Polysaccharides have buoyant densities in the range 1 ·6-2· 0 glml, depending on the nature of the supporting solvent (Dunstone 1969), and proteins usually have buoyant densities of less than 1· 4 g/ml (Cox and Schumaker 1961;Ifft and Vinograd 1962).…”

Section: (D) Density-gradient Ultracentrifugationmentioning

confidence: 99%

A Water-soluble Arabinogalactan-Peptide From Wheat Endosperm

Fincher¹,

Stone²

1974

Aust. Jnl. Of Bio. Sci.

148

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The water-soluble polymeric components of wheat endosperm have been extracted by two different procedures and their chemical composition studied in detail. Water extracts of wheat flour that had first been treated with hot 80% ethanol contained only 2% protein, but if the ethanol treatment was omitted up to 20% of the extracted polymeric fraction was low-molecular-weight non-dialysable protein material. Density-gradient ultracentrifugation in caesium chloride solutions indicated that most of this protein was free, whereas the 2% protein in the water extract of ethanol-treated flottr was firmly bound to a polysaccharide. This bound protein (a peptide) was characterized by high levels of hydroxyproline (16-20% of the amino acids present).Fractional precipitation of the water-soluble components with ammonium sulphate enabled the separation of a high-molecular-weight arabinoxylan (xylose : arabinose = 1·6 -1 . 9) and a lowmolecular-weight arabinogalactan (galactose :. arabinose =approx. 1· 5), the latter. being associated with the hydroxyproline-rich peptide.

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Section: (D) Density-gradient Ultracentrifugationmentioning

confidence: 99%

A Water-soluble Arabinogalactan-Peptide From Wheat Endosperm

Fincher¹,

Stone²

1974

Aust. Jnl. Of Bio. Sci.

148

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“…The methods of Meselson, Stahl & Vinograd (1957) and Schildkraut, Marmur & Doty (1962) were followed. An MSE analytical ultracentrifuge was used at approx.…”

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confidence: 99%

Mitochondrial deoxyribonucleic acid from Tetrahymena pyriformis and its kinetic complexity

Flavell

Jones

1970

Biochemical Journal

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1. Mitochondrial DNA from Tetrahymena pyriformis strain T has a buoyant density (ρ) of 1.685 compared with ρ1.688 for whole cell DNA. Mitochondrial preparations from T. pyriformis strain W show an enrichment of a light satellite (ρ1.686), although this is not obtained free from nuclear DNA (ρ1.692). 2. T. pyriformis mitochondrial DNA renatures rapidly and the kinetics of this process indicate a complexity of approx. 3×107 daltons. 3. The base-pairing in the renaturation product is of a precise nature, since the ‘melting’ temperature (80.5°C) is indistinguishable from that of the native DNA (80.5°C). 4. Centrifugation of mitochondrial DNA in an alkaline caesium chloride density gradient gives two bands, implying the separation of the complementary strands.

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“…DNA-base composition. One strain was examined in the National Collection of Type Cultures by equilibrium centrifugation in a neutral caesium chloride density gradient (Meselson, Stahl and Vinograd, 1957).…”

Section: Reduction Of Nitratementioning

confidence: 99%