Equilibrium Phase Behavior of Triblock Copolymer + Salt + Water Two-Phase Systems at Different Temperatures and pH

Silva, Luís Henrique Mendes da; Silva, Maria do Carmo Hespanhol da; Mesquita, Alessandra Rezende; Nascimento, Kelany S.; Coimbra, Jane Sélia dos Reis; Minim, Luís Antônio

doi:10.1021/je050104v

Cited by 35 publications

(8 citation statements)

References 28 publications

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“…The system formed by PEG 8000 presents higher biphasic region, decreasing the region when PEG 4000 is used and even more when PEG 1500 is applied. This result is coherent with literature [19] [20].…”

Section: Partition Process Analysissupporting

confidence: 93%

Aqueous Two-Phase Systems Applied to Partition Proteins from Goat Milk Whey In-Nature

Freire¹,

Pereira²

2016

ABB

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The proteins coming from the milk whey have numerous functional properties. Among the proteins with high bioactivity, α-lactoalbumin (α-La) and β-lactoglobulin (β-Lg) are present in large quantities in the milk whey. In the separation process of proteins, it is important to choose techniques which besides ensuring purity and high yield will not affect the molecule biological activity. The aqueous two-phase systems (ATS) have been utilized with success in the partition of these proteins, however, the studies were performed using protein in its pure form. Studies using milk whey in-nature and goat milk whey have not been found yet. In this context, the objective of this study was to evaluate the liquid liquid equilibrium of aqueous two-phase systems (ATS) in the partition of α-La and β-Lg from goat milk whey in-nature. Equilibrium data were performed considering ATS comprised of polyethylene glycol, potassium phosphate and water at 25˚C and pH 7.0. The influence of the polymer molecular weight and amount of goat milk whey in-nature on the partition coefficient of these proteins were assessed. The partition coefficient, selectivity, process yield and purity of α-lactoalbumin and β-lactoglobulin proteins were determined. The results showed that the separation technique by aqueous biphasic systems is applicable indicating high efficiency in the whey proteins separation process.

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Section: Partition Process Analysissupporting

confidence: 93%

Aqueous Two-Phase Systems Applied to Partition Proteins from Goat Milk Whey In-Nature

Freire¹,

Pereira²

2016

ABB

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“…A buffer composed of K 2 HPO 4 and C 6 H 8 O 7 was selected to prepare ABS at controlled pH, within a range from 3 to 8. Although Pluronic-based ABS have already been studied at distinct pH values (e.g., K 2 HPO 4 /KH 2 PO 4 at pH 7 and K 2 HPO 4 /KOH at pH 12), , the pH window previously available was quite narrow, limiting the purification/fractionation of biomolecules. The buffer here used affords a wider pH range and also offers a more benign nature due to the presence of the natural organic acid C 6 H 8 O 7 .…”

Section: Resultsmentioning

confidence: 99%

“…The use of (EO) x -(PO) y -(EO) x copolymers in ABS is not new, and their combination with conventional salts to induce liquid–liquid demixing in aqueous solution is well documented. Their phase separation phenomena were systematically studied considering distinct salts and Pluronics (varying both the molecular weight and EO/PO ratios) at distinct temperatures and pH. − Scarcer are the studies evaluating the possibility of creating ABS composed of Pluronics and other polymers (e.g., dextran). , Foreseeing their future application, some authors unveiled the extraction aptitude of Pluronic-based ABS for metallic ions, , peptides/proteins, ,, and vitamins, as well as their use for the extractive transformation of steroids . Liu et al reported the combined use of copolymers and cholinium-based ILs, focusing on the liquid–liquid equilibrium data and the impact of the copolymer nature on the ABS formation.…”

Section: Introductionmentioning

confidence: 99%

Using Ionic Liquids To Tune the Performance of Aqueous Biphasic Systems Based on Pluronic L-35 for the Purification of Naringin and Rutin

Silva

Carmo

Fernandes

et al. 2017

ACS Sustainable Chem. Eng.

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Aqueous biphasic systems (ABS) based on Pluronic L-35, a (EO) x -(PO) y -(EO) x triblock copolymer, were determined and applied in the separation of two structurally similar flavonoids (naringin and rutin). Two sets of phase formers were paired with Pluronic L-35, one comprising conventional salts/buffer and other including cholinium-based ionic liquids (ILs). It is shown that while the conventional salts induce an unbalanced and strong salting out leading to complete extraction of flavonoids to the same phase in most of the cases (84.7 ± 0.6% ≤ R NAR ≤ 100% and 53.2 ± 0.5% ≤ R RUT ≤ 99.7 ± 0.1% with selectivities ranging from 1 to 11.8), the cholinium-based ILs provide an enhanced extractive performance. Indeed, these novel cholinium ILs/ Pluronic L-35-based ABS allowed the manipulation of the affinity of both naringin and rutin to opposite phases, thus yielding a selective separation. The best results were achieved for the system using [Ch][Bic] as phase former (R NAR = 89.6 ± 0.3 and R RUT = 32 ± 2 with a selectivity of 18.9). An integrated approach based on the sequential implementation of Na 2 SO 4 /Pluronic L-35-(step 1) and [Ch][Bic]/Pluronic L-35-based (step 2) ABS was designed to purify the flavonoids from a complex synthetic mixture simulating natural extracts. Remarkably, glucose (the main contaminant) was removed during step 1 with an extraction efficiency of 60 ± 4% to the Na 2 SO 4 -rich phase, while step 2 has enabled the efficient separation of naringin from rutin.

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“…18−24 Ease of use, low costs, shorter time for phase splitting, and recycling of the system components are advantages of the technique which makes it a suitable method to extraction. 25,26 Aqueous two phase systems are composed of two immiscible aqueous phases at a certain concentration which are in equilibrium. When a clear interfacial boundary formed between the two phases, the separation is done where one phase is rich and the other phase is poor in solute.…”

Section: ■ Introductionmentioning

confidence: 99%

“…There are different technologies to remove the phenol derivates such as advance and electrochemical oxidation, , reverse osmosis, membrane separation and filtration, , chemical coagulation, biological degradation, − and adsorption. − However, there are many problems that need to be considered before the mentioned methods can be exerted in reality, such as preserving organism activity, regenerating the adsorbents, decreasing the consuming energy, increasing the removal efficiency, and so on. Among these methods, aqueous two-phase system (ATPS) can be a noteworthy replacement to other separation methods because of its economically lasting and environmentally secure properties. − Ease of use, low costs, shorter time for phase splitting, and recycling of the system components are advantages of the technique which makes it a suitable method to extraction. , Aqueous two phase systems are composed of two immiscible aqueous phases at a certain concentration which are in equilibrium. When a clear interfacial boundary formed between the two phases, the separation is done where one phase is rich and the other phase is poor in solute. − …”

Section: Introductionmentioning

confidence: 99%

Phase Diagram Study of Polymer-Salt-Based Aqueous Two-Phase Systems for Extraction of p-Nitrophenol

2020

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The separation and extraction of a phenolic compound called p-nitrophenol were studied by aqueous two-phase systems (ATPS). Solubility data were experimentally obtained at ambient temperature using dipotassium hydrogen phosphate, diammonium hydrogen phosphate, and sodium dihydrogen phosphate and polyethylene glycol polymers with molecular weights of 1000 and 4000 g/mol. The tie-lines were also obtained for solvation data. Then, experimental results were correlated with thermodynamic model UNIQUAC. Factors affecting the binodal curves such as salt types and temperature were also investigated. The salting-out ability of salts was studied, and the results showed that dipotassium hydrogen phosphate had the best result. By examining the effect of three temperatures of 298.15, 305.15, and 313.15 K on p-nitrophenol extraction, the percentage of extraction decreased by 6.05%. The percentage of p-nitrophenol extraction decreased with increasing molecular weight of the polymer and increased with increasing the polymer concentration. The highest obtained percentage of extraction was 96.85% in ATPS including dipotassium hydrogen phosphate (30% w/w) and polyethylene glycol 1000 (40% w/w).

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Equilibrium Phase Behavior of Triblock Copolymer + Salt + Water Two-Phase Systems at Different Temperatures and pH

Cited by 35 publications

References 28 publications

Aqueous Two-Phase Systems Applied to Partition Proteins from Goat Milk Whey In-Nature

Aqueous Two-Phase Systems Applied to Partition Proteins from Goat Milk Whey In-Nature

Using Ionic Liquids To Tune the Performance of Aqueous Biphasic Systems Based on Pluronic L-35 for the Purification of Naringin and Rutin

Phase Diagram Study of Polymer-Salt-Based Aqueous Two-Phase Systems for Extraction of p-Nitrophenol

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