Equilibrium phase behavior of the poly(ethylene glycol)/potassium phosphate/water two-phase system at 4.degree.C

Features of the acid protease partition in aqueous two-phase systems of polyethylene glycol–phosphate: Chymosin and pepsin

“…A partition coefficient of 2-4 has been reported for chymotrysinogen-A (MW 23,000) [10]. Also, the increase of [PEG] induced a significant increase in the K value, in constrast with previous reports which showed a partitioning decrease at increased [PEG] values [8].…”

“…The binodial diagram for PEG-potasium phosphaste systems were obtained at pH 7 from the method of turbidimetric titration as it was described in detail in [7,8].…”

Aspergillus oryzae alpha-amylase partition in potassium phosphate-polyethylene glycol aqueous two-phase systems

“…The system compositions, which are indicated in Table 1, were taken from the literature according to the binodial diagrams [5,9] for PEG 4000 and 8000, and those for PEG 2000 and 6000 were obtained in our laboratory (data not shown). The systems were prepared with PEG of four different molecular masses and four concentrations that correspond to the chosen tie lines.…”

Influence of the temperature and type of salt on the phase equilibrium of peg 1500 + potassium phosphate and peg 1500 + sodium citrate aqueous two-phase systems

“…Polymer + salt ATPS are particularly useful, since they present relatively low cost and low viscosity, which provide an efficient phase separation [11][12][13][14][15][16] . Equilibrium data for aqueous systems formed by PEG + sodium citrate and particularly for those composed of PEG + potassium phosphate are quite available [17][18][19][20][21][22][23][24][25][26][27][28] . However, there is nowadays a considerable demand for comprehensive studies with reference to the effect of operational conditions on the behavior of ATPS 29 .…”