Equilibrium of phosphatidylcholine-ergosterol in monolayers at the air-water interface

Janicka, K; Szeremeta, Michał; Petelska, Aneta D.

doi:10.1016/j.jct.2019.05.020

Cited by 2 publications

(5 citation statements)

References 39 publications

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“…The formed monolayer has three basic phase states: gaseous, liquid, and crystalline. Phosphatidylcholine formed stable monolayers, and its compression isotherm revealed a phase transition in a clear liquid expanded (LE)–liquid condensed (LC) phase, as previously described [ 31 , 32 ]. Molecules in the gas phase are loosely packed on the surface of the water and behave like a two-dimensional (2D) gas.…”

Section: Resultssupporting

confidence: 58%

“…For phosphatidylcholine (lecithin), this value is 64 Å 2 molecule −1 , which is in the range of the theoretical values [2,31,32]. The formed monolayer has three basic phase states: gaseous, liquid, and crystalline.…”

Section: Molecules 2020 25 X For Peer Review 4 Of 18mentioning

confidence: 67%

“…This surface is 12 Å 2 molecule −1 . phase transition in a clear liquid expanded (LE)-liquid condensed (LC) phase, as previously described [31,32]. Molecules in the gas phase are loosely packed on the surface of the water and behave like a two-dimensional (2D) gas.…”

Section: Molecules 2020 25 X For Peer Review 4 Of 18mentioning

confidence: 99%

“…The working conditions and experimental procedure: The surface tension was measured at the air/water interface (22 • C) using a homemade computer-controlled apparatus, as described in the previous papers [29,31].…”

Section: Monolayer Measurementsmentioning

confidence: 99%

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The Equilibria in Lipid–Lipoic Acid Systems: Monolayers, Microelectrophoretic and Interfacial Tension Studies

2020

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In this examination, we investigated the effect of lipoic acid (LA) on the properties of biological membrane models (monolayers, bilayers, and liposomes) formed from phosphatidylcholine (PC) or phosphatidylserine (PS) using the Langmuir, microelectrophoresis, and interfacial tension methods. The Langmuir technique allowed us to calculate the π–A isotherms and determine the molecular surface areas of pure and mixed monolayers. Using mathematical equations, we established that LA and the lipids formed complexes at a 1:1 ratio. The interfacial tension method was based on Young and Laplace’s equation. We assumed the formation of a 1:1 complex in the PC–LA system. Using the mathematical relationships, we derived the parameters characterizing the resulting complex, i.e., the surface occupied by the complex and the interfacial tension and stability constant of the formed complex. The microelectrophoretic method was used to determine the dependence of the zeta potential of the lipid membranes as a function of the pH (pH 2 to 10) of the electrolyte solution. The results indicate that modification of PC or PS membranes with LA affects changes in the zeta potential and the isoelectric point values.

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Section: Resultssupporting

confidence: 58%

Section: Molecules 2020 25 X For Peer Review 4 Of 18mentioning

confidence: 67%

Section: Molecules 2020 25 X For Peer Review 4 Of 18mentioning

confidence: 99%

Section: Monolayer Measurementsmentioning

confidence: 99%

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The Equilibria in Lipid–Lipoic Acid Systems: Monolayers, Microelectrophoretic and Interfacial Tension Studies

2020

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“…Since no accurate data of the stability constants for forming connections exist in the literature, our team, as a first, has determined these parameters and introduced them to the world. This represents an original contribution to the knowledge of the biochemistry and biophysics of lipid membranes [30][31][32][33][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

Equilibria in DPPC-Diosgenin and DPPC-Diosgenin Acetate Bilayer Lipid Membranes: Interfacial Tension and Microelectrophoretic Studies

et al. 2020

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Interactions between components of model lipid membranes (spherical lipid bilayers and liposomes) are investigated here. Parameters characterizing equilibria in the 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)-diosgenin (Dio) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)-diosgenin acetate (DAc) membrane systems have been determined. The interfacial tension measurement of spherical lipid bilayers was based on the Young-Laplace’s equation using a homemade computer-controlled device. We assume a 1:1 complex in the DPPC-Dio and DPPC-DAc membrane systems. The parameters A 3 − 1 , the surface concentration of lipid membranes formed from these complexes, γ 3 , the interfacial tension of such membranes, and, K, the constant stability of these complexes were calculated. Microelectrophoresis was used for examinations of the surface charge density of lipid membranes. The values were obtained here from electrophoretic mobility data applying Smoluchowsky’s equation. The effect of pH (pH ranged of 2 to 10) on the electrolyte solution and the compositions of the membranes was analyzed. The obtained results indicate that the modification of DPPC membranes with both Dio and DAc causes changes in surface charge density values and shifts of the isoelectric point.

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Equilibrium of phosphatidylcholine-ergosterol in monolayers at the air-water interface

Cited by 2 publications

References 39 publications

The Equilibria in Lipid–Lipoic Acid Systems: Monolayers, Microelectrophoretic and Interfacial Tension Studies

The Equilibria in Lipid–Lipoic Acid Systems: Monolayers, Microelectrophoretic and Interfacial Tension Studies

Equilibria in DPPC-Diosgenin and DPPC-Diosgenin Acetate Bilayer Lipid Membranes: Interfacial Tension and Microelectrophoretic Studies

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