Equilibrium data and thermodynamic studies of α-amylase partition in aqueous two-phase systems

Nascimento, Manuela Barreto do; Castro, Sérgio de Sousa; Veloso, Cristiane Martins; Fontan, Rafael da Costa Ilhéu; Nascimento, Danielle Jordana Soares do; Gandolfi, Olga Reinert Ramos; Sampaio, Vanessa Santos; Veríssimo, Lizzy Ayra Alcântara; Bonomo, Renata Cristina Ferreira

doi:10.1016/j.fluid.2018.02.005

Cited by 25 publications

(12 citation statements)

References 50 publications

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“…This behavior can be explained by the higher valence of the ions at pH 5. Following Nascimento et al ., higher valence is observed when pH is increased due to less protonation of anions. Anions with a higher valence are better salting‐out agents than anions with lower valence, since they have a greater capacity for hydration.…”

Section: Results and Discussonmentioning

confidence: 94%

Thermodynamics for curcumin (Curcuma longa L.) partitioning in the alcohol–salt aqueous two‐phase system

Batista

Gandolfi

Sampaio

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: Curcumin is a polyphenol used as a natural colorant or spice with pharmacological properties including analgesic, anti-inflammatory, anticarcinogenic and antioxidant. In this work the partition of curcumin was carried out in an aqueous two-phase system (ATPS) under different conditions. RESULTS: Liquid-liquid equilibrium data for the ATPS composed of 2-propanol + sodium phosphate + water were determined experimentally by the turbidimetric method at temperatures T = 288.15, 293.15, 298.15 and 303.15 K and pH values 4.0 and 5.0. The phase-forming and salting-out capability of the mentioned salt were discussed by determination of the exclusion volume effect (EEV) and salting-out coefficient. The partition of curcumin was carried out. In order to investigate the driving forces that govern the curcumin partition we conducted a thermodynamic study by isothermal titration calorimetry (ITC). The Gibbs free energy transfer ( tr G), transfer enthalpy variation ( tr H) and transfer entropy variation (T tr S) were measured during the dye partition for the systems at 298.15 K.CONCLUSION: The increase in the pH value promoted an increase in the salting-out effect as well as in the exclusion volume value. It was observed that the dye had a strong tendency to transfer to the alcohol-rich top phase, showing K p high values (13.725-112.764). Thermodynamic analysis indicated that curcumin partition in the ATPS at 298.15 K was entropically driven to the top phase. The high values of theoretical recovery, selectivity and partition coefficient obtained in this study show that the investigated systems may be a promising alternative for curcumin pre-concentration and purification.

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Section: Results and Discussonmentioning

confidence: 94%

Thermodynamics for curcumin (Curcuma longa L.) partitioning in the alcohol–salt aqueous two‐phase system

Batista

Gandolfi

Sampaio

et al. 2019

J of Chemical Tech & Biotech

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“…The nonlinear empirical Eq. (1) was used and correlated to the experimental data of the ([C 2 min]Cl) + PEG + water binodal (Souza et al 2015;Alvarez-Guerra et al 2016;Pimentel et al 2017;Song et al 2017;Tang et al 2017;Nascimento et al 2018).…”

Section: Determination Of the Phase Diagrams And Tie-linesmentioning

confidence: 99%

“…was solved using the solver of Excel (Wang et al 2010;Guo et al 2012;Han et al 2012;Pimentel et al 2017;Nascimento et al 2018):…”

Section: Determination Of the Phase Diagrams And Tie-linesmentioning

confidence: 99%

“…α-Amylase (α-1,4-glucan-4-glucanohydrolase: EC 3.2.1.1) constitutes an important group of biocatalysts for biotechnological applications, being distributed in plants, animals and microorganisms and those of microbial origin are predominant for commercial use. This enzyme promotes α-1,4 bond cleavage between adjacent units of glucose in starch, mainly producing maltose and maltodextrins (Michelin et al 2010;Singh and Kayastha 2014;Dey and Banerjee 2015;Nascimento et al 2018;Li et al 2019). There are many 1 3 industrial and biotechnological applications for α-amylases.…”

Section: Introductionmentioning

confidence: 99%

“…Shad et al (2018) evaluated the effects of various parameters such as PEG concentration and molecular mass, concentration of sodium citrate and NaCl on purification of α-amylase from white pitaya (Hylocereus undatus) peel. Nascimento et al (2018) studied the application of ATPS formed by polyethylene glycol 1500 g.mol −1 , sodium citrate/citric acid and water, at different pH (4.0, 5.0, 6.0 and 7.0) and temperatures T = (303.15 and 313.15) K for α-amylase partitioning. Tonova et al (2016) reported the study of α-amylase extraction, stripping and purification in ATPS based on hydrophobic and polar ionic liquids.…”

Section: Introductionmentioning

confidence: 99%

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Aqueous two-phase system (polyethylene glycol + ionic liquid) for extraction of α-amylase: phase diagrams, systems characterization and partition study

Gandolfi

Gonçalves

Batista

et al. 2020

Braz. J. Chem. Eng.

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The increasing demand for safe and effective methods for the separation of biological materials combining the advantageous properties of polyethylene glycol (PEG) (1500 g mol −1 or 4000 g mol −1) and ionic liquid has led to attractive and promising applications of the new aqueous biphasic systems. In this work, the liquid-liquid extraction process with aqueous two-phase systems (ATPS) was studied for α-amylase partitioning. The phase diagrams composed of PEG (1500 or 4000) g mol −1 + 1-ethyl-3-methylimidazolium chloride + water, were determined experimentally by the turbidimetric method at different temperatures T = (293.15, 303.15, 313.15 and 323.15) K. The effect of temperature and polymer molar mass on the phase diagrams, as well as the phase densities, refractive indexes and electrical conductivity were evaluated. Subsequently, the α-amylase partitioning was performed, where the partition coefficient was determined, besides the activity and selectivity. It was found that a temperature decrease and a polymer molar mass increase favored the formation of two phases. It was also found that α-amylase showed a preference for the bottom phase composed of PEG and the systems composed of PEG 1500 presented better results. Keyword Liquid-liquid extraction • Enzyme • Partition • Amylase Abbreviations ATPS Aqueous two-phase systems PEG Polyethylene glycol [C 2 min]Cl 1-Ethyl-3-methylimidazolium chloride TLL Tie-line lengths STL Tie-line slope U One enzyme activity unit releases 1 mmol of reducing sugar per mL of extract per minute, U mL −1 ART Total reducing sugars produced in the hydrolysis step, mg mL −1 VT Total volume used in the hydrolysis, mL V c Volume of sample used in the hydrolysis, mL R t Reaction time, min BSA Bovine serum albumin K p Protein partition coefficient C t Equilibrium concentration of the total protein in the top, mg mL −1 C b Equilibrium concentration of the total protein in the bottom, mg mL −1 K e Partition coefficient for enzyme activity A t Enzyme activity in the top, mg mL −1 A b Enzyme activity in the bottom, mg mL −1 Δ tr G Gibbs free energy variation, kJ mol −1 T Absolute temperature, K R Ideal gas constant, kJ mol −1 K −1

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Aqueous Two-Phase Systems: An Alternative Process for Industrial Dye Recovery

Alencar

Passos

Soriano

et al. 2020

Fate and Transport of Subsurface Pollutants

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Equilibrium data and thermodynamic studies of α-amylase partition in aqueous two-phase systems

Cited by 25 publications

References 50 publications

Thermodynamics for curcumin (Curcuma longa L.) partitioning in the alcohol–salt aqueous two‐phase system

Thermodynamics for curcumin (Curcuma longa L.) partitioning in the alcohol–salt aqueous two‐phase system

Aqueous two-phase system (polyethylene glycol + ionic liquid) for extraction of α-amylase: phase diagrams, systems characterization and partition study

Aqueous Two-Phase Systems: An Alternative Process for Industrial Dye Recovery

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