Equilibrium contrast CMR for the detection of amyloidosis in mice

Campbell, Adrienne E; Price, Anthony N.; Ellmerich, Stéphan; Simons, Paul; Al‐Shawi, Raya; Hawkins, Philip N.; Ordidge, Roger J.; Pepys, Mark B.; Moon, James; Lythgoe, Mark F.

doi:10.1186/1532-429x-13-s1-o60

Cited by 3 publications

(4 citation statements)

References 7 publications

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“…We acquired ASL data using a FAIR Look-Locker sequence with a single-slice spoiled gradient echo readout 35 . Perfusion maps were calculated 36 by assuming a blood-partition constant of 0.9 and longitudinal relaxation time of blood of 1900 ms 37 .…”

Section: Methodsmentioning

confidence: 99%

In vivo imaging of glucose uptake and metabolism in tumors

Walker‐Samuel

Ramasawmy

Torrealdea

et al. 2013

Nat Med

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Tumors display a greater reliance on glycolysis for energy production than normal tissues. We have developed a non-invasive method for imaging glucose uptake in vivo, which is based on magnetic resonance imaging, and allows the uptake of non-labeled glucose to be measured via the chemical exchange of protons between hydroxyl groups and water. This method differs from existing molecular imaging methods, as it permits detection of the delivery and uptake of a metabolically active compound at physiological quantities. We show that our technique, named glucose chemical exchange saturation transfer (glucoCEST), is sensitive to tumor glucose accumulation in colorectal tumor models, and can distinguish tumor types with differing metabolic characteristics and pathophysiology. The results of this study suggest that glucoCEST has potential as a useful and cost-effective method for characterizing disease and assessing response to therapy in the clinic.

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Section: Methodsmentioning

confidence: 99%

In vivo imaging of glucose uptake and metabolism in tumors

Walker‐Samuel

Ramasawmy

Torrealdea

et al. 2013

Nat Med

Self Cite

426

492

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“…Two i.p lines were used to administer either a bolus or an infusion of Gd.DTPA (Magnevist®, Schering, Berlin). Although, the kinetics of intravenous and i.p injection is different once equilibrium is reached the steady state is the same [26]. For this purpose the i.p route is more suitable and the dosing has been based on the late Gd i.p mouse dose described by Price A.N et al 2011 [28] with the subsequent infusion half the bolus dose over 1 hour as described for eqMRI by Campbell A.E et al 2011 [26].…”

Section: Methodsmentioning

confidence: 99%

“…Although the use of gadolinium for dynamic contrast enhancement (DCE) has had limited use in lung tumor imaging [24], it is conceivable that using a primed infusion, an equilibrium of Gd concentration between the vasculature and the interstitial spaces can be reached and sustained. This technique has been termed eqMRI and has been utilised to image myocardial fibrosis in humans [25], as well as systemic amyloidosis in preclinical murine models [26]. We hypothesised this technique may therefore improve the imaging of small lung tumors where equilibrium can provide continuous high concentrations of Gd enhancement.…”

Section: Introductionmentioning

confidence: 99%

Primed Infusion with Delayed Equilibrium of Gd.DTPA for Enhanced Imaging of Small Pulmonary Metastases

et al. 2013

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ObjectivesTo use primed infusions of the magnetic resonance imaging (MRI) contrast agent Gd.DTPA (Magnevist), to achieve an equilibrium between blood and tissue (eqMRI). This may increase tumor Gd concentrations as a novel cancer imaging methodology for the enhancement of small tumor nodules within the low signal-to-noise background of the lung.MethodsA primed infusion with a delay before equilibrium (eqMRI) of the Gd(III) chelator Gd.DTPA, via the intraperitoneal route, was used to evaluate gadolinium tumor enhancement as a function of a bolus injection, which is applied routinely in the clinic, compared to gadolinium maintained at equilibrium. A double gated (respiration and cardiac) spin-echo sequence at 9.4T was used to evaluate whole lungs pre contrast and then at 15 (representative of bolus enhancement), 25 and 35 minutes (representative of eqMRI). This was carried out in two lung metastasis models representative of high and low tumor cell seeding. Lungs containing discrete tumor nodes where inflation fixed and taken for haematoxylin and eosin staining as well as CD34 staining for correlation to MRI.ResultsWe demonstrate that sustained Gd enhancement, afforded by Gd equilibrium, increases the detection of pulmonary metastases compared to bolus enhancement and those tumors which enhance at equilibrium are sub-millimetre in size (<0.7 mm2) with a similar morphology to early bronchoalveolar cell carcinomas.ConclusionAs Gd-chelates are routinely used in the clinic for detecting tumors by MRI, this methodology is readily transferable to the clinic and advances MRI as a methodology for the detection of small pulmonary tumors.

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“…Moreover, combining pre‐ and post‐contrast agent injection T 1 measurements enables quantification of local contrast agent concentrations . Finally, non‐contrast‐enhanced cardiac T 1 mapping has proven useful in distinguishing infarcted from healthy myocardium in both mice and humans , as well as in detection of human myocardial fibrosis and murine cardiac amyloidosis .…”

Section: Imaging Strategiesmentioning

confidence: 99%

Contrast‐enhanced MRI of murine myocardial infarction – Part II

et al. 2012

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Mouse models are increasingly used to study the pathophysiology of myocardial infarction in vivo. In this area, MRI has become the gold standard imaging modality, because it combines high spatial and temporal resolution functional imaging with a large variety of methods to generate soft tissue contrast. In addition, (target-specific) MRI contrast agents can be employed to visualize different processes in the cascade of events following myocardial infarction. Here, the MRI sequence has a decisive role in the detection sensitivity of a contrast agent. However, a straightforward translation of clinically available protocols for human cardiac imaging to mice is not feasible, because of the small size of the mouse heart and its extremely high heart rate. This has stimulated intense research in the development of cardiac MRI protocols specifically tuned to the mouse with regard to timing parameters, acquisition strategies, and ECG- and respiratory-triggering methods to find an optimal trade-off between sensitivity, scan time, and image quality. In this review, a detailed analysis is given of the pros and cons of different mouse cardiac MR imaging methodologies and their application in contrast-enhanced MRI of myocardial infarction.

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Equilibrium contrast CMR for the detection of amyloidosis in mice

Cited by 3 publications

References 7 publications

In vivo imaging of glucose uptake and metabolism in tumors

In vivo imaging of glucose uptake and metabolism in tumors

Primed Infusion with Delayed Equilibrium of Gd.DTPA for Enhanced Imaging of Small Pulmonary Metastases

Contrast‐enhanced MRI of murine myocardial infarction – Part II

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