Equilibrium constants for thiol-disulfide interchange reactions: a coherent, corrected set

Lees, Watson J.; Whitesides, George M.

doi:10.1021/jo00055a016

Cited by 201 publications

(170 citation statements)

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“…These results indicated that psammaplin A inhibited the growth of bacteria probably by inhibiting DNA gyrase (256). Since the significant antimicrobial activity of psammaplin A (152) was disclosed, the antibacterial activities against 40 drug- Bastadins-1 to-7 (204)(205)(206)(207)(208)(209)(210)(211) were isolated because of the potent activity against Grampositive and Gram-negative bacteria, but data were not provided (129). Bastadin-13 showed a minimum inhibitory concentration of 6 μg/mL against Bacillus subtilis, while 34-sulfatobastadin-13 showed no activity at 50 μg/mL, suggesting the free phenolic group at C-34 is required for the antimicrobial activity (141 …”

Section: A Antimicrobial Activitymentioning

confidence: 96%

“…The majority of the bastadins were isolated from the marine sponge Ianthella basta (class Demospongiae, order Verongida, family Inthellidae) with several obtained from I. flabelliformis, I. quadrangulata, Ianthella sp., and Psammaplysilla purpurea. Bastadins 1-7 (204)(205)(206)(207)(208)(209)(210)(211) were isolated from the Australian sponge I. basta, as antimicrobial agents against Gram positive organisms in 1980 (128,129). Their structures were determined by spectroscopic methods and alcoholic KOH hydrolysis.…”

Section: Oxime-histamines-ianthellinmentioning

confidence: 99%

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The Marine Bromotyrosine Derivatives

Peng¹,

Jing²,

Hamann³

2005

The Alkaloids: Chemistry and Biology

101

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Section: A Antimicrobial Activitymentioning

confidence: 96%

Section: Oxime-histamines-ianthellinmentioning

confidence: 99%

The Marine Bromotyrosine Derivatives

Peng¹,

Jing²,

Hamann³

2005

The Alkaloids: Chemistry and Biology

101

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“…Values for E m were determined by fitting titration data to the Nernst equation, E h ¼ E m þ (RT/nF)(ln([ox]/ [red]), with RT/F ¼ 25.693 mV and the value of n ¼ 2, as expected for a two-electron transfer process, using Kaleidagraph (Synergy Software) (Hutchinson and Ort, 1995;Bick et al, 2001). Calculation of the E m value is based on the E m value of DTT at pH 7.0 (À327 mV) (Lees and Whitesides, 1993).…”

Section: Redox Titrationsmentioning

confidence: 99%

Thiol-Based Regulation of Redox-Active Glutamate-Cysteine Ligase from Arabidopsis thaliana

et al. 2007

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Glutathione biosynthesis is a key component in the network of plant stress responses that counteract oxidative damage and maintain intracellular redox environment. Using a combination of mass spectrometry and site-directed mutagenesis, we examined the response of Arabidopsis thaliana glutamate-cysteine ligase (GCL) to changes in redox environment. Mass spectrometry identified two disulfide bonds (Cys186-Cys406 and Cys349-Cys364) in GCL. Mutation of either Cys-349 or Cys-364 to a Ser reduced reaction rate by twofold, but substitution of a Ser for either Cys-186 or Cys-406 decreased activity by 20-fold and abrogated the response to changes in redox environment. Redox titrations show that the regulatory disulfide bond has a midpoint potential comparable with other known redox-responsive plant proteins. Mutation of Cys-102, Cys-251, Cys-349, or Cys-364 did not alter the response to redox environment, indicating that modulation of activity depends on the Cys186-Cys406 disulfide bond. In vivo analysis of GCL in Arabidopsis root extracts revealed that multiple oxidative stresses altered the distribution of oxidized (active) and reduced (inactive) enzyme and that this change correlated with increased GCL activity. The thiol-based regulation of GCL provides a posttranslational mechanism for modulating enzyme activity in response to in vivo redox environment and suggests a role for oxidative signaling in the maintenance of glutathione homeostasis in plants.

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“…E h was calculated using the Nernst equation, E h = E m + (RT/nF) ln([DTT ox ]/[DTT red ]), with n = 2 for a twoelectron reaction, RT/F = 25.693 mV, and an E m, DTT value of 2327 mV (Lees and Whitesides, 1993). In brief, samples were incubated in 300 mL of 100 mM Tricine-KOH, pH 7.7, with 10 mM total DTT red /DTT ox for 90 min at room temperature and assayed for GLYK activity with assay 1 above.…”

Section: Redox Titrationmentioning

confidence: 99%

An Autoinhibitory Domain Confers Redox Regulation to Maize Glycerate Kinase

Bartsch

Mikkat²,

Hagemann

et al. 2010

Plant Physiology

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Glycerate 3-kinase (GLYK) is the terminal enzyme of the photorespiratory cycle in plants and many cyanobacteria. For several C 4 plants, notably grasses of the NADP-malic enzyme (ME) subtype, redox regulation of GLYK has been reported, but the responsible molecular mechanism is not known. We have analyzed the enzyme from the NADP-ME C 4 plant maize (Zea mays) and found that maize GLYK, in contrast to the enzyme from C 3 plants and a dicotyledonous NADP-ME C 4 plant, harbors a short carboxy-terminal extension. In its oxidized (night) form, a disulfide bridge is formed between the two cysteine residues present in this extra domain, and GLYK activity becomes inhibited. Cleavage of this bond by thioredoxin f produces the fully active thiol form, releasing autoinhibition. Fusion of the maize GLYK redox-regulatory domain to GLYK from C 3 plants confers redox regulation to these otherwise unregulated enzymes. It appears that redox regulation of GLYK could be an exclusive feature of monocotyledonous C 4 plants of the NADP-ME type, in which linear electron transport occurs only in the mesophyll chloroplasts. Hence, we suggest that GLYK, in addition to its function in photorespiration, provides glycerate 3-phosphate for the accelerated production of triose phosphate and its export from the mesophyll. This could facilitate the activation of redoxregulated Calvin cycle enzymes and the buildup of Calvin cycle intermediates in the bundle sheath of these particular C 4 plants during the dark/light transition.

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Equilibrium constants for thiol-disulfide interchange reactions: a coherent, corrected set

Cited by 201 publications

References 0 publications

The Marine Bromotyrosine Derivatives

The Marine Bromotyrosine Derivatives

Thiol-Based Regulation of Redox-Active Glutamate-Cysteine Ligase from Arabidopsis thaliana

An Autoinhibitory Domain Confers Redox Regulation to Maize Glycerate Kinase

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