Equilibrium centrifugation of DNA, RNA and poliovirus in density gradients of cesium oxalate and cesium acetate

Zolotor, Laurence; Engler, Reto

doi:10.1016/0005-2787(67)90653-3

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein

1967

DOI: 10.1016/0005-2787(67)90653-3

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Equilibrium centrifugation of DNA, RNA and poliovirus in density gradients of cesium oxalate and cesium acetate

Laurence Zolotor

Reto Engler

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1969

1979

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Cited by 14 publications

(1 citation statement)

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“…The dialysis step in the fixation procedure was found to remove almost all the acid-soluble material, so that radioactivities of fractions from this and the subsequent gradient were counted directly. BIOCHEMICAL SOCIETY TRANSACTIONS strengths of the concentrated solutions of the caesium salts used as gradient solutes (see Spirin, 1969;Zolotor & Engler, 1967) dissociated the RNA and protein of unfixed particles. [Very recently, Kempf et al (1972) have shown that the least-dense ribonucleoprotein particles will specifically band in sucrose-2H20 gradients.…”

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confidence: 99%

The Use of Metrizamide for the Isopycnic Gradient Fractionation of Unfixed Ribonucleoprotein Particles

Mullock

Hinton

1973

Biochemical Society Transactions

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The Use of Metrizamide for the Isopycnic Gradient Fractionation of Unfixed Ribonucleoprotein Particles

Mullock

Hinton

1973

Biochemical Society Transactions

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Volume changes accompanying the thermal denaturation of deoxyribonucleic acid. I. Denaturation at neutral pH

Chapman

Sturtevant

1969

Biopolymers

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SynopsisDilatometric measurements were made to determine the change in apparent specific volume (p of DNA reaulting from thermal denaturation in neutral solution. (p increased continuously with temperature in the range 10-85°C. No deviations from a monotonically rising curve were observed in the (p versus temperature profile in the region of the melting temperature. The results are interpreted in terms of a partial loas of the preferentially bound DNA hydration shell. The nature of the well known buoyant density difference between native and denatured DNA was investigated by evaluating the densities in a series of cesium salt gradients at constant temperature. Extrapolation of the results to zero water activity indicates that the partial specific volumes of anhydrous native and denatured DNA are equal. The density difference at nonzero water activities is attributed to decreased hydration in the denatured state. The absence of a related change in 0 accompanyihg the denaturation in the dilatometric experiments suggests that the probable volume change associated with loss of bound water during denaturation is accompanied by other compensatory volume effects. The pmible nature of these volume effects is discussed.

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Sucrose interference in the assay of enzymes and protein

Hinton

Hartman

1969

Analytical Biochemistry

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Equilibrium centrifugation of DNA, RNA and poliovirus in density gradients of cesium oxalate and cesium acetate

Cited by 14 publications

References 12 publications

The Use of Metrizamide for the Isopycnic Gradient Fractionation of Unfixed Ribonucleoprotein Particles

The Use of Metrizamide for the Isopycnic Gradient Fractionation of Unfixed Ribonucleoprotein Particles

Volume changes accompanying the thermal denaturation of deoxyribonucleic acid. I. Denaturation at neutral pH

Sucrose interference in the assay of enzymes and protein

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