Equilibrium adsorption of proteins

“…Problems relevant to the reversibility of protein adsorption at the gas-liquid interfaces were discussed in [46,47]. In particular, it was disclosed that, at low surface pressures (below 15 mN/m), proteins almost do not desorb from a deposited monolayer into the pure solvent (subphase), and, at higher surface pressures, the desorption rate is low and it is not controlled by diffusion.…”

Section: Kinetics Of Desorptionmentioning

confidence: 99%

Equilibrium and Dynamic Characteristics of Protein Adsorption Layers at Gas-Liquid Interfaces: Theoretical and Experimental Data

Fainerman

Miller

2005

Colloid J

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Within the framework of a nonideal two-dimensional solution model, equations are derived for the state of a surface layer, adsorption isotherms, and the distribution function of adsorbed protein molecules with respect to their states characterized by different molar surface areas. The derived equations satisfactorily describe the known experimental dependences obtained for equilibrium adsorption layers of some proteins (serum albumin, β -casein, and β -lactoglobulin): the dependences of the surface pressure on concentration and adsorption, the surface layer thickness on adsorption, and the limiting high-frequency elastic modulus of an adsorption layer on the surface pressure. All dependences for a given protein are described by the same set of parameters of the theoretical model. It is shown that the kinetics of protein adsorption studied by dynamic tensiometry, ellipsometry, and the radiotracer technique is consistent with the diffusion model comprising the Ward-Tordai equation and the set of equations describing the equilibrium. The kinetics of protein desorption from the adsorption layer at a liquid-fluid interface is analyzed. The kinetics of β -lactoglobulin desorption is shown to be described by the barrier mechanism.

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“…Gonzalez and Mac-solutions at pH 7 and 5.5 were prepared with mono-and Ritchie measured the rate of desorption of BSA from an disodium phosphate and were used within 2 weeks after air-water interface and found that the energy barrier to de-preparation. Throughout the experiments, ultrapure Milli-Q sorption is very high (17). Hunter et al (18) observed that water was used.…”

Section: Introductionmentioning

confidence: 99%