2005
DOI: 10.1161/01.res.0000172568.49367.f8
|View full text |Cite
|
Sign up to set email alerts
|

Equilibrative Nucleoside Transporter 1 Expression Is Downregulated by Hypoxia in Human Umbilical Vein Endothelium

Abstract: Abstract-Reduced oxygen level (hypoxia) induces endothelial dysfunction and release of the endogenous nucleoside adenosine. Human umbilical vein endothelium (HUVEC) function in an environment with 3% to 5% O 2 and exhibit efficient adenosine membrane transport via human equilibrative nucleoside transporters 1 (hENT1). We studied whether adenosine transport and hENT1 expression are altered by hypoxia in HUVEC. Hypoxia (0 to 24 hours, 2% and 1% O 2 ) reduced maximal hENT1-adenosine transport velocity (V max ) an… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

8
107
2

Year Published

2006
2006
2018
2018

Publication Types

Select...
8
2

Relationship

3
7

Authors

Journals

citations
Cited by 72 publications
(117 citation statements)
references
References 42 publications
8
107
2
Order By: Relevance
“…These results could partially explain the decrease in dFdC uptake in Panc-1 cells. This is consistent with recent reports stating that hypoxia can repress the expression of hENT1 and/or hENT2 in different cell lines [i.e., human microvascular endothelial cells (Eltzschig et al, 2005) and human umbilical vein endothelial cells (Casanello et al, 2005)]. Clinical reports indicate that decreased expression of hENT1 can be correlated with treatment failure of dFdC in cancers, including mantle cell lymphoma (Marcé et al, 2006), pancreatic cancer (Spratlin et al, 2004;Giovannetti et al, 2006), and non-small-cell lung cancer (Achiwa et al, 2004).…”
Section: Discussionsupporting
confidence: 91%
“…These results could partially explain the decrease in dFdC uptake in Panc-1 cells. This is consistent with recent reports stating that hypoxia can repress the expression of hENT1 and/or hENT2 in different cell lines [i.e., human microvascular endothelial cells (Eltzschig et al, 2005) and human umbilical vein endothelial cells (Casanello et al, 2005)]. Clinical reports indicate that decreased expression of hENT1 can be correlated with treatment failure of dFdC in cancers, including mantle cell lymphoma (Marcé et al, 2006), pancreatic cancer (Spratlin et al, 2004;Giovannetti et al, 2006), and non-small-cell lung cancer (Achiwa et al, 2004).…”
Section: Discussionsupporting
confidence: 91%
“…Endothelial cells were isolated from the human umbilical vein by digestion with collagenase (0.25 mg/mL) and then cultured (37°C, 5 % CO 2 ) in medium 199 (M199) as previously described [26]. All experiments were performed in duplicate, after overnight serum deprivation.…”
Section: Huvec Culturementioning
confidence: 99%
“…ENTl activity and expression in human umbilical vein endothelial cells (HUVEC) have been shown to be decreased under J. Physiol. Biochem., 63 (1), 2007 hypoxia, which might contribute to increase extracellular adenosine levels (6). In the basal forebrain, ENTl has also been suggested to be implicated in the increase of extracellular adenosine levels associated with sleep deprivation, thus resulting in the inhibition of wake-active neurons and a decrease of cortical activity (34).…”
Section: Purinergic Regulationmentioning
confidence: 99%