Equibiaxial cyclic stretch stimulates fibroblasts to rapidly remodel fibrin

Balestrini, Jenna L.; Billiar, Kristen L.

doi:10.1016/j.jbiomech.2005.10.025

Cited by 72 publications

(53 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, we hypothesize that the cell-derived collagen matrix in fibrin TECs at day 6 is not yet sufficient to appropriately respond to this stimulus and that delaying the onset of mechanical stimulation might prove beneficial. However, other studies have shown that peak strains for approximately 10% are required to increase collagen production and mechanical properties of fibrin-based TECs, 30,31,65 suggesting that the 2.4% peak strain used in our study may have been too low to stimulate beneficial effects. By contrast, mechanical stimulation improved tenogenic expression patterns in collagen TECs, as they retained near normal expression for approximately 14 days in culture.…”

Section: Figmentioning

confidence: 66%

“…(2) The initial protein concentrations of collagen and fibrin were not equal but selected to be consistent with numerous previous studies. 22,26,28,30,31,67 However, other studies have illustrated that varying the concentrations of these materials can affect parameters such as collagen synthesis, cell proliferation, TEC contraction, and biomechanics. 40,55,68 Systematically varying the concentration of collagen or fibrin used in future studies will be necessary to evaluate whether the effects measured here have been influenced by these differences.…”

Section: Figmentioning

confidence: 99%

“…[25][26][27] Cells in fibrin gels also appear to recapitulate aspects of native tendon development in vitro 28,29 and exhibit improved cell-derived collagen organization in response to mechanical stimulation. 30,31 However, research has not yet been conducted that directly compares how collagen and fibrin materials affect the ability of TLPs to respond to mechanical stimuli and produce an organized ECM.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fibrin Gels Exhibit Improved Biological, Structural, and Mechanical Properties Compared with Collagen Gels in Cell-Based Tendon Tissue-Engineered Constructs

Breidenbach

Dyment

et al. 2015

Tissue Engineering Part A

View full text Add to dashboard Cite

The prevalence of tendon and ligament injuries and inadequacies of current treatments is driving the need for alternative strategies such as tissue engineering. Fibrin and collagen biopolymers have been popular materials for creating tissue-engineered constructs (TECs), as they exhibit advantages of biocompatibility and flexibility in construct design. Unfortunately, a few studies have directly compared these materials for tendon and ligament applications. Therefore, this study aims at determining how collagen versus fibrin hydrogels affect the biological, structural, and mechanical properties of TECs during formation in vitro. Our findings show that tendon and ligament progenitor cells seeded in fibrin constructs exhibit improved tenogenic gene expression patterns compared with their collagen-based counterparts for approximately 14 days in culture. Fibrin-based constructs also exhibit improved cell-derived collagen alignment, increased linear modulus (2.2-fold greater) compared with collagen-based constructs. Cyclic tensile loading, which promotes the maturation of tendon constructs in a previous work, exhibits a material-dependent effect in this study. Fibrin constructs show trending reductions in mechanical, biological, and structural properties, whereas collagen constructs only show improved tenogenic expression in the presence of mechanical stimulation. These findings highlight that components of the mechanical stimulus (e.g., strain amplitude or time of initiation) need to be tailored to the material and cell type. Given the improvements in tenogenic expression, extracellular matrix organization, and material properties during static culture, in vitro findings presented here suggest that fibrin-based constructs may be a more suitable alternative to collagen-based constructs for tissue-engineered tendon/ligament repair.

show abstract

Section: Figmentioning

confidence: 66%

Section: Figmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fibrin Gels Exhibit Improved Biological, Structural, and Mechanical Properties Compared with Collagen Gels in Cell-Based Tendon Tissue-Engineered Constructs

Breidenbach

Dyment

et al. 2015

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…Cells under high stress generally assemble stiffer or stronger matrices (Isenberg and Tranquillo 2003;Balestrini and Billiar 2006). These changes appear to be due in part to effects of forces on gene expression and in part because of more direct effects on matrix assembly.…”

Section: Signaling Pathways and Gene Expressionmentioning

confidence: 99%

Integrins and Extracellular Matrix in Mechanotransduction

Schwartz¹

2010

Cold Spring Harbor Perspectives in Biology

549

432

View full text Add to dashboard Cite

Integrins bind extracellular matrix fibrils and associate with intracellular actin filaments through a variety of cytoskeletal linker proteins to mechanically connect intracellular and extracellular structures. Each component of the linkage from the cytoskeleton through the integrin-mediated adhesions to the extracellular matrix therefore transmits forces that may derive from both intracellular, myosin-generated contractile forces and forces from outside the cell. These forces activate a wide range of signaling pathways and genetic programs to control cell survival, fate, and behavior. Additionally, cells sense the physical properties of their surrounding environment through forces exerted on integrin-mediated adhesions. This article first summarizes current knowledge about regulation of cell function by mechanical forces acting through integrin-mediated adhesions and then discusses models for mechanotransduction and sensing of environmental forces.

show abstract

“…They have been widely used in the last decade for a variety of tissue engineering applications in adipose [21], cardiovascular [22,23], ocular [24,25], muscle [26,27], liver [28,29], skin [30,31], cartilage [32,33], and bone [34,35] tissues. Moreover, fibrin hydrogels are able to promote angiogenesis [36,37].…”

Section: Fibrinmentioning

confidence: 99%

Artificial Ovary

Amorim¹

2016

Gonadal Tissue Cryopreservation in Fertility Preservation

View full text Add to dashboard Cite

Survival rates of many malignant diseases are steadily improving, but for patients of childbearing age, fertility restoration often becomes a vital concern after disease remission. In women, treatments such as chemo/radiotherapy can be very harmful to the ovaries, causing loss of both endocrine and reproductive functions. When gonadotoxic treatment cannot be delayed, ovarian tissue cryobanking is the only way of preserving fertility. However, this technique is not advisable for patients with certain types of cancer, since there is a risk of reintroducing malignant cells present in the cryopreserved tissue. For these patients, a safer alternative could be transplantation of isolated preantral follicles back to their natural environment. To encapsulate and protect isolated follicles, a transplantable artificial ovary needs to be created. The main goal of the artificial ovary is to mimic the natural organ, and for this, it should be composed of a matrix that encapsulates and protects not only the isolated follicles but also autologous ovarian cells and bioactive factors, which are necessary for follicle survival and development. The aim of this chapter is to describe this new technology, its indications, advantages, and the different approaches to create it.

show abstract

Equibiaxial cyclic stretch stimulates fibroblasts to rapidly remodel fibrin

Cited by 72 publications

References 27 publications

Fibrin Gels Exhibit Improved Biological, Structural, and Mechanical Properties Compared with Collagen Gels in Cell-Based Tendon Tissue-Engineered Constructs

Fibrin Gels Exhibit Improved Biological, Structural, and Mechanical Properties Compared with Collagen Gels in Cell-Based Tendon Tissue-Engineered Constructs

Integrins and Extracellular Matrix in Mechanotransduction

Artificial Ovary

Contact Info

Product

Resources

About