"Equator Assessment of Nanoparticles Using the Design- Expert Software"

Abdul, Ahad Hindustan; Bala, Anusha Golla; Chintaginjala, Haranath; Manchikanti, Sai Priyanka; Kamsali, Akhil; Dasari, Rahul Raghav

doi:10.37285/ijpsn.2020.13.1.5

Cited by 14 publications

(5 citation statements)

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“…The membrane and receptor phase were in contact for an hour at a temperature of 37 ± 0.5 °C before the experiment. Samples (0.5 mL) were obtained at specified intervals throughout the receptor phase’s 8 h (0.5, 1, 2, 4, 6, and 8 h) and assessed by HPLC at 288 nm, while the cell was replenished with an equivalent quantity of freshly made PBS. ,− …”

Section: Methodsmentioning

confidence: 99%

Developing Nanosuspension Loaded with Azelastine for Potential Nasal Drug Delivery: Determination of Proinflammatory Interleukin IL-4 mRNA Expression and Industrial Scale-Up Strategy

Mehmood

Shahid

Abbas³

et al. 2023

ACS Omega

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In order to increase bioavailability and intranasal absorbance, the current work set out to create azelastine nasal spray based on nanosuspension. Chondroitin was utilized as a polymer to prepare azelastine nanosuspension through the precipitation procedure. A size of 500 nm and a polydispersity index of 0.276 with a negative potential (−20 mV) were achieved. X-ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy, thermal analysis including differential scanning calorimetry and thermogravimetric analysis, in vitro release, and diffusion studies were used to characterize the optimized nanosuspension. MTT assay was used to assess the viability of the cells, and hemolysis assay was used to assess the blood compatibility. Using RNA extraction and reverse transcription polymerase chain reaction, the levels of the anti-inflammatory cytokine IL-4, which is most closely related to cytokines in allergic rhinitis, were measured in mouse lungs. The drug dissolution and diffusion study indicated 2.0-fold increase compared to pure reference sample. Therefore, the azelastine nanosuspension could be suggested as a practical and simple nanosystem for intranasal delivery with improved permeability and bioavailability. The outcome obtained in this study indicated that azelastine nanosuspension has great potential to treat allergic rhinitis as intranasal treatment.

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Section: Methodsmentioning

confidence: 99%

Developing Nanosuspension Loaded with Azelastine for Potential Nasal Drug Delivery: Determination of Proinflammatory Interleukin IL-4 mRNA Expression and Industrial Scale-Up Strategy

Mehmood

Shahid

Abbas³

et al. 2023

ACS Omega

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“…Porosity levels are often quite high in microsponges that are made using the solvent diffusion approach. BET analysis was utilized to assess the respective surface areas, pore sizes, and volumes of the unloaded and loaded surfaces [38]. The BET surface area of the unloaded microspheres was S BET (m 2 /g) 6.31 ± 0.22, and the surface area of the loaded microspheres was 5.80 ± 0.09.…”

Section: Nitrogen Adsorption-desorption Analysismentioning

confidence: 99%

Microsponge-Based Gel Loaded with Immunosuppressant as a Simple and Valuable Strategy for Psoriasis Therapy: Determination of Pro-Inflammatory Response through Cytokine IL-2 mRNA Expression

Mehmood,

Shahid,

ul Huq

et al. 2023

Gels

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Tacrolimus (TL) is a topical calcineurin inhibitor immunosuppressive drug widely used to manage various skin disorders. Herein, we report a TL-loaded microsphere gel formulation with severe atopic dermatitis effects that are required to manage skin disorders. The current study adopted a modified emulsion solvent evaporation technique to synthesize TL-loaded microspheres, which were further converted into gels for skin use. Characterization of the synthesized formulation was performed by differential dynamic light scattering, scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray crystallography, Brunauer–Emmett–Teller (BET) analysis, differential scanning calorimetry, and drug release. A Franz diffusion cell was used to study the diffusion of TL for up to 8 h at pH 6.8 and 5.5. Evaluation of cell viability was determined by MTT assay and showed higher IC50 values compared to the plain drug. RNA extraction, real-time polymerase chain reaction (RT–PCR), and reverse transcription were also performed to determine the expression levels of the anti-inflammatory cytokine IL-2. Particle size determination was performed by a zeta sizer, and the TL microsphere size was 1745 ± 70 nm with a good polydispersity (0.337 ± 0.12). The drug entrapment efficiency was also very good at 60% ± 10, and the drug release was 93.9% ± 3.5 within 8 h. An in vitro diffusion study of the formulation also showed improved permeability at both pH values (4.5 and 5.5). The findings of the hemolytic tests demonstrated that TL-MG at concentrations of 50, 100, and 200 mg/mL did not produce any hemolysis. A dose-dependent pattern of cytotoxicity was found during the cell viability assay, with an IC50 value of 787.55 ± 12.78 µg/mL. There was a significant decrease in the IL-2 level in the TL-MG group compared to the other groups. TL-MG microspheres were nontoxic carriers for tacrolimus delivery, with greater loading capacity, a significant release profile, and enhanced cellular uptake with improved permeability.

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“…The variables and their ranges for MG screening were created using a total of 9 experimental designs. 12,13 The ingredients of copious MG are listed in Table 1.…”

Section: Experimental Designmentioning

confidence: 99%

Impact of Pistacia lentiscus Plant Gum on Particle Size and Swelling Index in Central Composite Designed Amoxycillin Trihydrate Mucoadhesive Microspheres

Mundarinti¹,

Ahad²

2023

Ind. J. Pharm. Edu. Res.

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Objectives:The ambition of this study is to find the mucoadhesive assets of Pistacia lentiscus plant gum (mastic gum) by combining it into mucoadhesive microspheres with Amoxicillin Trihydrate (ATH). Significance: Due to its short stomach residence duration, ATH is efficient against H. pylori but can be improved by creating Mucoadhesive Microspheres (MMS) that keep Amoxicillin trihydrate in the stomach. In this study, Pistacia lentiscus plant gum has been exposed to have gastroprotective and H. pylori eradication possessions in inclusion to being able to increase mucoadhesion. Methods and Results: The study was performed to find the influence of the mucilage amount on particle size and swelling index. MMS of ATH (9 batches) were made with carbopol 934P (C-934P) and changeable extents of Pistacia lentiscus plant gum commonly called Mastic Gum (MG). A central composite design to find the influence of factors (MG and C-934 P levels) on particle size and swelling index as an output. The batches were evaluated for their physical limitations, ATH level, and liberation as part of amiability research. The particle size was seen as 35.2±0.3-48.1±0.6μm. In batch B-1, the particle size was the smallest compared to the larger size in B-8. The overall formula was +47.04+0.6500A+1.93B-0.1750AB-0.6167A²-8.47B². The swelling ranged from 58.6±1.3-74.3±1.6 and it was also observed to increase with the polymer level and the formula was +54.21+1.40A+6.25B+0.4750AB-0.5667A²+2.78B². Conclusion: The search establishes that the particle size and the swelling index depend on Pistacia lentiscus plant gum levels. The investigation revealed that ATH was consistently released in a regulated pattern and that encapsulation efficiency, mucosal adhesion, ATH content, and other limitations were considered good. When prepared as an MMS, ATH can achieve good gastric-specific drug delivery when enriched with MG and C-934P. The microspheres had smooth surfaces and were found to be spherical by scanning electron microscopy.

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"Equator Assessment of Nanoparticles Using the Design- Expert Software"

Cited by 14 publications

References 0 publications

Developing Nanosuspension Loaded with Azelastine for Potential Nasal Drug Delivery: Determination of Proinflammatory Interleukin IL-4 mRNA Expression and Industrial Scale-Up Strategy

Developing Nanosuspension Loaded with Azelastine for Potential Nasal Drug Delivery: Determination of Proinflammatory Interleukin IL-4 mRNA Expression and Industrial Scale-Up Strategy

Microsponge-Based Gel Loaded with Immunosuppressant as a Simple and Valuable Strategy for Psoriasis Therapy: Determination of Pro-Inflammatory Response through Cytokine IL-2 mRNA Expression

Impact of Pistacia lentiscus Plant Gum on Particle Size and Swelling Index in Central Composite Designed Amoxycillin Trihydrate Mucoadhesive Microspheres

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