Equations for calculation of chromatographic figures of merit for ideal and skewed peaks

Foley, Joe P.; Dorsey, John G.

doi:10.1021/ac00255a033

Cited by 606 publications

(207 citation statements)

References 20 publications

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“…In case of increased peak asymmetry values ( > 1.25) the accuracy of the calculated plate numbers will rapidly decrease [30]. Also from our data the asymmetry factors, asf, were calculated at 10% of the peak heights…”

Section: Reversed-phase Modementioning

confidence: 67%

Evaluation of mixed-mode stationary phases in liquid chromatography for the separation of charged and uncharged oligomer-like model compounds

Eleveld

Claessens

Ammerdorffer

et al. 1994

Journal of Chromatography A

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Section: Reversed-phase Modementioning

confidence: 67%

Evaluation of mixed-mode stationary phases in liquid chromatography for the separation of charged and uncharged oligomer-like model compounds

Eleveld

Claessens

Ammerdorffer

et al. 1994

Journal of Chromatography A

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“…Accurate estimations of m 1 and m 2 ¢ may be difficult under such conditions. On the basis of an exponentially modified Gaussian model, Foley and Dorsey 12 proposed some equations for calculating the chromatographic figures of merit for ideal and skewed peaks. The values of m 1 and m 2 ¢ of the peaks were calculated by applying the following equations:…”

Section: Discussionmentioning

confidence: 99%

Effect of the Type of Organic Modifiers on Mass Transfer in Reversed-Phase Liquid Chromatography

Miyabe

Takeuchi

1998

ANAL. SCI.

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Methanol/water and acetonitrile/water mixtures have generally been used as a mobile phase in reversedphase liquid chromatography (RP-LC). Chromatographic separations are significantly affected by the mobile-phase conditions, such as the type and composition of the organic modifiers. A number of studies have been made on the influence of the mobile-phase conditions on the retention behavior in RP-LC in connection with the specification of the separation mechanism and with the establishment of optimization strategies for chromatographic conditions.It is also essential to analyze the chromatographic processes from the viewpoints of mass-transfer kinetics and the thermodynamic properties as well as the retention behavior in order to understand the separation mechanism in RP-LC. However, there have not been very many kinetic studies on mass-transfer phenomena in RP packing materials [1][2][3][4] , in contrast to the extremely extensive studies on chromatographic retention. The authors have studied mass-transfer phenomena in RP-LC using mainly methanol/water and octadecylsilyl (ODS)-silica gel as mobile and stationary phases. 5-8Pulse-response experiments were made under various conditions of the solutes as well as the stationary and mobile phases. Their type, composition, and concentration were changed. Some items of information about the retention behavior, mass-transfer rates, and thermodynamic properties in RP-LC were obtained.This paper is concerned with the influence of the type of organic modifiers in the mobile phases on the chromatographic properties in RP-LC. The information about the retention behavior, mass-transfer rates, and thermodynamic properties were determined by using a mixture of acetonitrile and water (70/30, v/v) and an ODS-silica gel as a mobile and stationary phase. In a previous paper 9 , the authors reported on results of a similar study using alkylbenzenes and p-alkylphenols as solutes. In this study, the characteristics of the RP-LC system were analyzed in more detail using more solutes than before. In particular, it was attempted to correlate the surface diffusion coefficient (D s ) with various physical parameters of the solutes. The results in 70 vol% acetonitrile were compared with those in 70 vol% methanol. On the basis of the results, a few estimation procedures of D s have been proposed. In this paper, the mass-transfer and concentration phenomena of solutes from a bulk mobile phase to the surface of ODS-silica gel is represented by the word "adsorption" in a wide sense. The mechanism of the mass transfer, i.e., "partition or adsorption", is not defined. Experimental Column and reagentsSeveral properties of an ODS column (YMC) and the experimental conditions are given in Table 1. An ODS column packed with relatively large ODS-silica gel particles was used in order to make it easy to quantitatively analyze the influence of the mass-transfer rates on the peak spreading. The volumetric composition of acetonitrile was 70%, which was selected as a typical value of mobile-phase compositio...

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“…The peak asymmetry factor (F asy ) was calculated by drawing a perpendicular line from the peak apex to the baseline and measuring the front (a) and back (b) peak widths at 10% height [15]: F asy = b/a. The chromatographic efficiency was determined using the following equation: N = (41.7 × (t r /W 0.1 ) 2 )/(b/a + 1.25), where W 0.1 represents the peak width at 10% height [16].…”

Section: Calculationmentioning

confidence: 99%

HPLC and UPLC Analyses of Acetoin in Bacterial Culture Fluid

Федорова¹,

Kivero²,

Geraskina³

et al. 2015

Acta Chromatographica

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Summary.A simple and rapid ultra-performance liquid chromatographic (UPLC) method for analyzing acetoin in bacterial culture fluid was developed and validated for the first time. The samples were separated using an Acquity BEH C18 column (2.1 mm × 100 mm, 1.7 μm particle size) and isocratic elution with 30 mM phosphoric acid-1% acetonitrile as the mobile phase. A photodiode array detector (PDA) was used. The run time was 6 min, and the detection limit was 2.11 × 10 −4 mg mL −1 . The UPLC method was compared with high-pressure liquid chromatography (HPLC) for acetoin analysis. The proposed UPLC method is highly sensitive and was successfully applied to the analysis of acetoin in bacterial culture fluid.

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Equations for calculation of chromatographic figures of merit for ideal and skewed peaks

Cited by 606 publications

References 20 publications

Evaluation of mixed-mode stationary phases in liquid chromatography for the separation of charged and uncharged oligomer-like model compounds

Evaluation of mixed-mode stationary phases in liquid chromatography for the separation of charged and uncharged oligomer-like model compounds

Effect of the Type of Organic Modifiers on Mass Transfer in Reversed-Phase Liquid Chromatography

HPLC and UPLC Analyses of Acetoin in Bacterial Culture Fluid

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