Epstein-barr virus replication studies and their application to vector design

Abstract

Vectors containing elements of the Epstein-Barr virus genome are used primarily to maintain cloned DNA inserts as plasmids in mammalian cells. However, Epstein-Bar-virus-based vectors have also been valuable tools in the hands of those studying the life cycle of Epstein-Barr virus. In this article, we discuss those characteristics of Epstein-Barr virus and its life cycle that have been used in vector construction and describe methods that are particularly applicable to the use of Epstein-Barr-virus-based vectors.

Stabilization of transgenes delivered by recombinant adenovirus vectors through extrachromosomal replication

“…Since adenovirus-based vectors cannot replicate in non-permissive cells, the number of viral genomes per cell declines as the cells divide. In contrast, EBV-based episomes that are capable of replicating in human cells maintain an approximately steady number of episomal genomes per cell [20,21]. As reported previously [13], in the absence of selection, cells lose EBV-based episomes at a rate of approximately 5% per cell division, whereas for non-replicating vectors, this rate is theoretically at least ten-fold higher.…”

Characterization of Epstein-Barr virus type 1 nuclear antigen 3C sequence patterns of nasopharyngeal and gastric carcinomas in northern China

“…Epstein-Barr virus (EBV) is a human herpesvirus that infects over 90% of the human population and has been linked with numerous human cancers, including Burkitt's lymphoma, nasopharyngeal carcinoma (NPC), gastric carcinoma (GC), and breast carcinoma [1][2][3][4].…”