Epstein–Barr virus miR-BART4-3p regulates cell proliferation, apoptosis, and migration by targeting AXL in gastric carcinoma

Zhao, Menghe; Liu, Wen; Zhang, Yan; Liu, Juanjuan; Song, Hui; Luo, Bing

doi:10.1007/s11262-021-01882-5

Cited by 4 publications

(4 citation statements)

References 33 publications

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“…In this experiment, 5 µL of the exosome/LMP1 complex from an NPC patient serum (SNPC) was used in a culture medium without FBS to test cell proliferation, as described previously [ 13 ]. The controls used were exosomes isolated from healthy individuals (EC-SNF), with or without FBS.…”

Section: Methodsmentioning

confidence: 99%

Suppression of Nasopharyngeal and Gastric Tumor Growth in a Mouse Model by Antibodies to Epstein–Barr Virus LMP1 Protein

et al. 2023

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The study aimed to investigate the antitumor efficacy of anti-LMP1 antibodies in EBV-positive nasopharyngeal and stomach cell lines and xenograft models. The study also examined the NF-κB expression and cell cycle activation of NPC-serum-exosome-associated LMP1. Anti-LMP1 antibody treatment before or during cell implantation prevented tumor growth in nude mice. A small dose of antibodies resulted in complete tumor regression for at least three months after the tumors had grown in size. The consumption of antigen–antibody complexes by tumor cells limited tumor growth. In vitro experiments showed that anti-LMP1 antibodies killed EBV-positive NPC- or GC-derived epithelial cell lines and EBV-positive human B-cell lines but not EBV-negative cell lines. Treatment with anti-LMP1 reduced NF-κB expression in cells. The animal model experiments showed that anti-LMP1 inhibited and prevented NPC- or GC-derived tumor growth. The results suggest that LMP1 antibody immunotherapy could cure nasopharyngeal cancer, EBV-positive gastric carcinoma, and EBV-associated lymphomas. However, further validation of these findings is required through human clinical trials.

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Section: Methodsmentioning

confidence: 99%

Suppression of Nasopharyngeal and Gastric Tumor Growth in a Mouse Model by Antibodies to Epstein–Barr Virus LMP1 Protein

et al. 2023

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“…Additional EBV-encoded miRNAs contribute to immune escape through diverse mechanisms. For instance, miR-BART4-3p exerts a multifaceted influence on gastric carcinoma cells by regulating proliferation, apoptosis, and migration ( 50 ). Similarly, miR-BART4-5p promotes immune evasion by downregulating proapoptotic proteins, thereby reducing apoptosis in gastric cancer cells ( 51 ).…”

Section: The Elusive Escape Artist: Unraveling Ebv’s Art Of Immune Ev...mentioning

confidence: 99%

Epstein-Barr virus: the mastermind of immune chaos

Silva,

Alves,

Pontes

2024

Front. Immunol.

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The Epstein-Barr virus (EBV) is a ubiquitous human pathogen linked to various diseases, including infectious mononucleosis and multiple types of cancer. To control and eliminate EBV, the host’s immune system deploys its most potent defenses, including pattern recognition receptors, Natural Killer cells, CD8+ and CD4+ T cells, among others. The interaction between EBV and the human immune system is complex and multifaceted. EBV employs a variety of strategies to evade detection and elimination by both the innate and adaptive immune systems. This demonstrates EBV’s mastery of navigating the complexities of the immunological landscape. Further investigation into these complex mechanisms is imperative to advance the development of enhanced therapeutic approaches with heightened efficacy. This review provides a comprehensive overview of various mechanisms known to date, employed by the EBV to elude the immune response, while establishing enduring latent infections or instigate its lytic replication.

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“…In EBVaGC cells, EBV-encoded miRNAs modulate epithelialmesenchymal transition (EMT), affecting intercellular adhesion and cell motility and deformability, leading to alterations in the migration capability of the tumor cells (32). Meng-He Zhao and colleagues revealed that miR-BART4-3P targeted the AXL gene directly to promote the apoptosis and suppress the proliferation and migration of GC cells via downstream effectors of EMT, including E-cadherin, Vimentin, Zinc Finger E-box Binding Homeobox 1 (ZEB1), and phospho-AKT (P-AKT) (33). Besides, Juanjuan Liu et al reported that miR-BART1-5p restrained GC cells proliferation and migration by targeting GCNT3 (core 2b-1, 6-acetylglucosaminyltransferase) via modulating the expressions of E-cadherin, N-cadherin, and Vimentin (34).…”

Section: Migrationmentioning

confidence: 99%

The role of EBV-encoded miRNA in EBV-associated gastric cancer

et al. 2023

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Epstein-Barr virus (human herpesvirus 4, EBV) is a linear double-stranded DNA virus that infects over 90% of the population worldwide. However, our understanding of EBV’s contribution to tumorigenesis of EBV-associated GC (EBVaGC) remains incomplete. Recent advancements in EBVaGC research have highlighted that EBV-encoded microRNAs (miRNAs) play prominent roles in critical cellular processes such as migration, cell cycle, apoptosis, cell proliferation, immune response, and autophagy. Notably, the largest group of EBV-encoded miRNAs, known as BamHI-A rightward transcripts (BARTs), exhibit bidirectional effects in EBVaGC. For instance, they present both anti-apoptotic and pro-apoptotic functions and enhance chemosensitivity while also conferring resistance to 5-fluorouracil. Despite these findings, the comprehensive mechanisms through which miRNAs contribute to EBVaGC are yet to be fully elucidated. In this work, we summarize the current evidence of the roles of miRNA in EBVaGC, particularly with the application of multi-omic techniques. Additionally, we discuss the application of miRNA in EBVaGC in retrospective analyses and provide novel perspectives on the use of miRNA in EBVaGC in translational medicine.

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Epstein–Barr virus miR-BART4-3p regulates cell proliferation, apoptosis, and migration by targeting AXL in gastric carcinoma

Cited by 4 publications

References 33 publications

Suppression of Nasopharyngeal and Gastric Tumor Growth in a Mouse Model by Antibodies to Epstein–Barr Virus LMP1 Protein

Suppression of Nasopharyngeal and Gastric Tumor Growth in a Mouse Model by Antibodies to Epstein–Barr Virus LMP1 Protein

Epstein-Barr virus: the mastermind of immune chaos

The role of EBV-encoded miRNA in EBV-associated gastric cancer

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