Epstein-Barr virus markers in a series of Burkitt's lymphomas from the West Nile District, Uganda

Geser, Anton; Lenoir, Gilbert; Anvret, Maria; Bornkamm, Georg W.; Klein, George; Williams, Edward H.; Wright, Dennis H.; de-Thé, G

doi:10.1016/0277-5379(93)90009-t

Cited by 44 publications

(28 citation statements)

References 26 publications

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“…Some Burkitt's lymphoma B cells, such as the Daudi line, are infected with Epstein-Barr virus (EBV) (11), and under certain circumstances this may alter the cell's phenotype (9,16,18,33). The consequences include increased expression of the antiapoptosis protein, Bcl-2, leading to apoptosis resistance, and decreased expression of the CD77 (Gb3-Cer) receptor on cell surfaces (14,15,33).…”

Section: Apoptogenic Activity Of Stx1 or Stx2 Holotoxins Or Their Clomentioning

confidence: 99%

Cloned Shiga Toxin 2 B Subunit Induces Apoptosis in Ramos Burkitt's Lymphoma B Cells

2002

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The Shiga toxins (Stx1 and Stx2), produced by Shigella dysenteriae type 1 and enterohemorrhagic Escherichia coli, consist of one A subunit and five B subunits. The Stx1 and Stx2 B subunits form a pentameric structure that binds to globotriaosylceramide (Gb3-Cer) receptors on eukaryotic cells and promotes endocytosis. The A subunit then inhibits protein biosynthesis, which triggers apoptosis in the affected cell. In addition to its Gb3-Cer binding activity, the data in the following report demonstrate that the Stx2 B pentamer induces apoptosis in Ramos Burkitt's lymphoma B cells independently of A subunit activity. Apoptosis was not observed in A subunit-free preparations of the Stx1 B pentamer which competitively inhibited Stx2 B pentamer-mediated apoptosis. The pancaspase inhibitor, Z-VAD-fmk, prevented apoptosis in Ramos cells exposed to the Stx2 B subunit, Stx1 or Stx2. Brefeldin A, an inhibitor of the Golgi transport system, also prevented Stx2 B subunit-mediated apoptosis. These observations suggest that the Stx2 B subunit must be internalized, via Gb3-Cer receptors, to induce Ramos cell apoptosis. Moreover, unlike the two holotoxins, Stx2 B subunitmediated apoptosis does not involve inhibition of protein biosynthesis. This study provides further insight into the pathogenic potential of this family of potent bacterial exotoxins.

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Section: Apoptogenic Activity Of Stx1 or Stx2 Holotoxins Or Their Clomentioning

confidence: 99%

Cloned Shiga Toxin 2 B Subunit Induces Apoptosis in Ramos Burkitt's Lymphoma B Cells

2002

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“…Previous studies carried out on BL biopsies from endemic regions of Africa demonstrated that more than 95% carried EBV (Geser et al, 1983). Typing of the EBV present in African BL biopsies revealed about a third to be infected with B-type EBV (Zimber et al, 1986;Young et al, 1987).…”

Section: Discussionmentioning

confidence: 95%

Heterogeneity within the Epstein-Barr virus nuclear antigen 2 gene in different strains of Epstein-Barr virus

Sengupta

Aedes

Moss

et al. 1994

Journal of General Virology

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“…These tumour types are histologically indistinguishable; however, in endemic (e) BL, which is found in equatorial Afri ca and Papua New Guinea, 97% of tumours are associat ed with EBV [3], whereas sporadic (s) BL, which occurs worldwide at a lower incidence, is only associated with EBV in around 12% of cases. In vitro, cell lines can be established from both types of BL, and these grow in sus pension culture as small, non-activated B cells.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Viral Gene Expression and Genome Persistence in CD23-Positive and -Negative Subpopulations of Epstein-Barr-Virus-lnfected BL2 Cells

Doyle¹,

Crawford²

1994

Intervirology

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Epstein-Barr virus (EBV) infection of the EBV-negative Burkitt lymphoma (BL) cell line BL2 has been studied in vitro, to assess the efficiency of stable infection and the state of the viral genome in these cells. Expression of the EBV nuclear antigen complex (EBNA 1-6) was detected by 24 h post-infection, but expression of the latent membrane protein (LMP) and of the B cell activation antigen CD23 were delayed until 8 days post-infection, and were then only detectable in a subpopulation of EBNA-positive cells. Separation of cells into CD23-positive and -negative fractions 8 days after infection showed that the CD23-positive cells expressed EBNA 1-6 and LMP and contained viral episomes, whereas the CD23-negative cells were EBNA 1-6 positive, LMP negative and had very low levels of episomal viral genome. Cloning of the CD23-positive and -negative populations showed that EBV infection in the majority of clones was lost, with only 29 out of the 164 investigated still EBNA positive after 6 months of culture. Stable infection correlated with expression of EBNA 1-6, LMP, CD23 and the presence of viral episomes. One clone stably expressed EBNA 1-6 and LMP in the absence of detectable linear or episomal viral genome; this clone was assumed to have a viral genome integrated into the cellular chromosomes. Thus integration of the viral genome following in vitro infection of BL2 cells is a rare event.

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Epstein-Barr virus markers in a series of Burkitt's lymphomas from the West Nile District, Uganda

Cited by 44 publications

References 26 publications

Cloned Shiga Toxin 2 B Subunit Induces Apoptosis in Ramos Burkitt's Lymphoma B Cells

Cloned Shiga Toxin 2 B Subunit Induces Apoptosis in Ramos Burkitt's Lymphoma B Cells

Heterogeneity within the Epstein-Barr virus nuclear antigen 2 gene in different strains of Epstein-Barr virus

Analysis of Viral Gene Expression and Genome Persistence in CD23-Positive and -Negative Subpopulations of Epstein-Barr-Virus-lnfected BL2 Cells

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