Epstein‐Barr virus infection and expression in oral lesions

Raab‐Traub, Nancy; Webster‐Cyriaque, Jennifer

doi:10.1111/j.1601-0825.1997.tb00352.x

Cited by 29 publications

(19 citation statements)

References 31 publications

(34 reference statements)

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“…The etiopathogenesis of HL is associated with the replication of EBV in superficial epithelial cells [6,9]. It was initially described as being exclusive to AIDS patients [7,8,10].…”

Section: Introductionmentioning

confidence: 99%

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Detection of the Epstein–Barr Virus (EBV) by In situ Hybridization as Definitive Diagnosis of Hairy Leukoplakia

Braz‐Silva

Rezende

Ortega

et al. 2008

Head and Neck Pathol

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Histopathological findings in cases of hairy leukoplakia (HL) are not exclusive to this lesion. A total of 36 tissue samples from patients previously diagnosed with HL based solely on morphological aspects were used in this study. Our purpose was to confirm the presence of EpsteinBarr virus (EBV) in these tissue samples by in situ hybridization (ISH), and to compare the detection of EBV with specific histopathological findings observed in each case. Among the 36 specimens, 80.55% were EBV positive, confirming the previous clinical and histhophatological diagnosis. None of the histopathological findings analyzed correlated with the presence or absence of EBV. This shows that a definitive diagnosis of HL cannot be established based on histopathological findings alone. Because there are many important implications on the establishment of definitive diagnosis of HL, the detection of EBV by ISH is obligatory.

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“…The etiopathogenesis of HL is associated with the replication of EBV in superficial epithelial cells [6,9]. It was initially described as being exclusive to AIDS patients [7,8,10].…”

Section: Introductionmentioning

confidence: 99%

“…EBV is the causative agent of infectious mononucleosis, and is associated with hairy leukoplakia (HL) and certain lymphoid and epithelial cancers such as Burkitt's lymphoma, immunoblastic lymphoma, Hodgkin's lymphoma, and nasopharyngeal cancer [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Detection of the Epstein–Barr Virus (EBV) by In situ Hybridization as Definitive Diagnosis of Hairy Leukoplakia

Braz‐Silva

Rezende

Ortega

et al. 2008

Head and Neck Pathol

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“…EBV is the etiological agent of infectious mononucleosis and is also associated with various human malignancies, including Burkitt's lymphoma, nasopharyngeal carcinoma, non-Hodgkin's disease, AIDS immunoblastic lymphomas, and lymphoproliferative disease (3,63). Infection of the oropharyngeal epithelium is predominantly a lytic type of infection with the production of progeny virus (33,61,63,73). Infection of human primary B lymphocytes by EBV transforms them into continuously proliferating lymphoblastoid cell lines (LCLs) in vitro (11,29).…”

mentioning

confidence: 99%

Modulation of Histone Acetyltransferase Activity through Interaction of Epstein-Barr Nuclear Antigen 3C with Prothymosin Alpha

Cotter

Robertson

2000

Molecular and Cellular Biology

109

106

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The Epstein-Barr virus (EBV) nuclear antigen 3C (EBNA3C) is essential for EBV-dependent immortalization of human primary B lymphocytes. Genetic analysis indicated that amino acids 365 to 992 are important for EBV-mediated immortalization of B lymphocytes. We demonstrate that this region of EBNA3C critical for immortalization interacts with prothymosin alpha (ProT␣), a cellular protein previously identified to be important for cell division and proliferation. This interaction maps to a region downstream of amino acid 365 known to be involved in transcription regulation and critical for EBV-mediated transformation of primary B lymphocytes. Additionally, we show that EBNA3C also interacts with p300, a cellular acetyltransferase. This interaction suggests a possible role in regulation of histone acetylation and chromatin remodeling. An increase in histone acetylation was observed in EBV-transformed lymphoblastoid cell lines, which is consistent with increased cellular gene expression. These cells express the entire repertoire of latent nuclear antigens, including EBNA3C. Expression of EBNA3C in cells with increased acetyltransferase activity mediated by the EBV transactivator EBNA2 results in down-modulation of this activity in a dose-responsive manner. The interactions of EBNA3C with ProT␣ and p300 provide new evidence implicating this essential EBV protein EBNA3C in modulating the acetylation of cellular factors, including histones. Hence, EBNA3C plays a critical role in balancing cellular transcriptional events by linking the biological property of mediating inhibition of EBNA2 transcription activation and the observed histone acetyltransferase activity, thereby orchestrating immortalization of EBV-infected cells.Epstein-Barr Virus (EBV) is a human gammaherpesvirus predominantly infecting epithelial cells of the oropharynx and human primary B lymphocytes (41,63). EBV is the etiological agent of infectious mononucleosis and is also associated with various human malignancies, including Burkitt's lymphoma, nasopharyngeal carcinoma, non-Hodgkin's disease, AIDS immunoblastic lymphomas, and lymphoproliferative disease (3,63). Infection of the oropharyngeal epithelium is predominantly a lytic type of infection with the production of progeny virus (33,61,63,73). Infection of human primary B lymphocytes by EBV transforms them into continuously proliferating lymphoblastoid cell lines (LCLs) in vitro (11,29). Recent studies have demonstrated that EBV utilizes two major cellular signaling pathways for transforming B cells, the NOTCH1 signaling pathway and the TNF signaling pathway (6, 34, 57).After initial infection of B lymphocytes, EBV typically establishes a latent infection with the expression of 11 viral transcripts (41, 63). These genes are the six EBV nuclear antigens (EBNAs), three latent membrane proteins (LMPs), and the EBV early RNAs (41). Only a selected number of these genes are necessary for EBV-mediated immortalization of B lymphocytes (65). EBNA2, EBNA3A, EBNA3C, and LMP1 are essential for EBV-induced immorta...

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“…Analysis of the pattern confirms the presence of EBV, distinguishes monoclonal from oligoclonal or polyclonal EBV infection, and identifies active viral replication by the presence of linear EBV genomes (in addition to episomal genomes of latent infection). Most EBV-associated tumors demonstrate monoclonal EBV infection, whereas oral hairy leukoplakia represents high levels of virus replication and reinfection, resulting in polyclonal infections within the lesion [17].…”

Section: Dot Blotting and Southern Blottingmentioning

confidence: 99%

Virologic diagnosis, viral monitoring, and treatment of epstein-barr virus infectious mononucleosis

Jenson

2004

Curr Infect Dis Rep

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Epstein-Barr virus (EBV) is the cause of infectious mononucleosis and is associated with severe infections in immunocompromised patients. EBV is also causally linked with several human malignancies. The heterophile antibody test and EBV-specific antibody tests remain the principal means of diagnosis of initial infection in otherwise healthy patients. Enzyme-linked immunosorbent assays have replaced the traditional immunofluorescence assays for EBV-specific antibodies. Several newer molecular diagnostic tests have become available that facilitate accurate monitoring of infection. The role of these tests for patients with uncomplicated infectious mononucleosis is limited, although these tests are being increasingly used to monitor the state and level of EBV replication for severe infections and among immunocompromised patients. Antiviral therapy has a limited, short-term effect on oropharyngeal shedding but has proven ineffective for the clinical manifestations of infectious mononucleosis. Patients with selected complications frequently benefit from short-term corticosteroid therapy.

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Epstein‐Barr virus infection and expression in oral lesions

Cited by 29 publications

References 31 publications

Detection of the Epstein–Barr Virus (EBV) by In situ Hybridization as Definitive Diagnosis of Hairy Leukoplakia

Detection of the Epstein–Barr Virus (EBV) by In situ Hybridization as Definitive Diagnosis of Hairy Leukoplakia

Modulation of Histone Acetyltransferase Activity through Interaction of Epstein-Barr Nuclear Antigen 3C with Prothymosin Alpha

Virologic diagnosis, viral monitoring, and treatment of epstein-barr virus infectious mononucleosis

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