Epstein-Barr virus induces aggressive lymphoproliferative disorders of human B cell origin in SCID/hu chimeric mice.

Cannon, Martin J.; Pisa, Pavel; Fox, Robert I.; Cooper, Neil R.

doi:10.1172/jci114573

Cited by 138 publications

(69 citation statements)

References 17 publications

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“…The observed variation of human IgG levels in reconstituted mice is consistent with previous studies (13,21,22). Mice with high IgG levels (> 15 mg/ml) often developed lymphomas, which are commonly found in SCID mice reconstituted with PBL from EBV-positive donors (23). The time course ofEBV-induced lymphomas varies depending on the percentage ofEBV-positive cells in the total lymphocyte population and the total injected dose ( 12).…”

Section: Introductionsupporting

confidence: 88%

Development of pemphigus vulgaris-like lesions in severe combined immunodeficiency disease mice reconstituted with lymphocytes from patients.

Juhász¹,

Lazarus²,

Murphy³

et al. 1993

J. Clin. Invest.

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Pemphigus vulgaris is an autoimmune blistering disease that is induced by binding of antibodies to a 130/85-kD protein complex on epidermal keratinocytes. An in vivo experimental model of this disease was developed by reconstituting severe combined immunodeficiency (SCID) mice with 1-10 X 10' PBL from patients with naturally occurring pemphigus vulgaris. Of 49 reconstituted mice, 34 (69%) produced human IgG levels of > 0.1 mg/ml. Circulating anti-pemphigus antibodies were found in 20 of the 34 successfully reconstituted mice; 44% of these animals had deposits of human IgG in their own skin after it was traumatized by either heat or cold. Spontaneous pemphigus vulgaris-like blisters associated with human IgG deposits were rarely found in mouse skin. By contrast, allogeneic human skin grafted to 10 of 12 mice before reconstitution with patients' PBL developed pemphigus vulgaris-like lesions containing human IgG deposits. These results demonstrate that SCID mice can serve as a model of an antibody-mediated human autoimmune skin disease. (J. Clin. Invest. 1993.92:2401-2407

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Section: Introductionsupporting

confidence: 88%

Development of pemphigus vulgaris-like lesions in severe combined immunodeficiency disease mice reconstituted with lymphocytes from patients.

Juhász¹,

Lazarus²,

Murphy³

et al. 1993

J. Clin. Invest.

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“…In 1988, Mosier et al (7), in attempting to reconstitute these mice with human B and T cells, reported the development of EBV-positive B-cell lymphomas in mice inoculated with peripheral blood lymphocytes from EBV-seropositive individuals. Several other groups have also reported similar findings (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19). Mosier and colleagues have since extended their observations to show that the peripheral blood of some EBV-seropositive donors is more likely to form tumors in SCID mice than that of others (20).…”

supporting

confidence: 65%

Epstein-Barr Virus–Infected Marmoset Cells Do Not Form Lymphomas in Mice with Severe Combined Immunodeficiency

et al. 1994

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“…More recently, this observation has been extended by other investigators who have reported lymphomas of human type in SCID mice after i.p. transfer of hu-PBLs from EBV-seropositive donors (Bankert et al, 1989;Rowe et al, 1991) or by transfection of human lymphoid cells from EBV antibody-seronegative donors with EBV within the SCID mouse (Cannon et al, 1990;Okano et al, 1990;Purtilo et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Role of Epstein-Barr virus and interleukin 6 in the development of lymphomas of human origin in SCID mice engrafted with human tonsillar mononuclear cells

Nadal¹,

Albini²,

Schläpfer³

et al. 1992

Journal of General Virology

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Mice with severe combined immunodeficiency were inoculated intraperitoneally with 50x 106 human tonsillar mononuclear cells (hu-TMCs) from EpsteinBarr virus (EBV) antibody seropositive or seronegative human subjects. Between 5 and 11 weeks later, 29.4% (10/34) of mice injected with hu-TMCs from EBV seropositive donors, but none of 34 animals receiving hu-TMCs from EBV seronegative donors, developed intraabdominal and/or intrathoracic tumours (P, 0.002). By means of in situ hybridization using alpha satellite DNA from human chromosome 17, all tumours produced after cell transfer from EBV seropositive donors were identified to be of human origin. Histologically the tumours resembled large cell lymphomas; the EBV genome was detected by in situ hybridization and EBV nuclear antigen by immunofluorescence in these tumours. The tumours were polyor oligoclonal, and stained for human IgG and IgM, and less frequently IgA and IgD. Serum levels of human immunoglobulin in animals developing human tumours were significantly higher than in reconstituted mice without tumours and the sera exhibited polyoligo-or monoclonality in immunoelectrophoresis. Human interleukin 6 was detected in the serum of six of 10 animals with human lymphomas, but not in any animals without human lymphoma.

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Epstein-Barr virus induces aggressive lymphoproliferative disorders of human B cell origin in SCID/hu chimeric mice.

Cited by 138 publications

References 17 publications

Development of pemphigus vulgaris-like lesions in severe combined immunodeficiency disease mice reconstituted with lymphocytes from patients.

Development of pemphigus vulgaris-like lesions in severe combined immunodeficiency disease mice reconstituted with lymphocytes from patients.

Epstein-Barr Virus–Infected Marmoset Cells Do Not Form Lymphomas in Mice with Severe Combined Immunodeficiency

Role of Epstein-Barr virus and interleukin 6 in the development of lymphomas of human origin in SCID mice engrafted with human tonsillar mononuclear cells

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