EPR spectroscopy as a probe of metal centres in biological systems

Hagen, Wilfred R.

doi:10.1039/b608163k

Cited by 96 publications

(70 citation statements)

References 69 publications

(68 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…155 The use of these approaches in studies of metalloproteins should also benefit from application of the other SSNMR approaches described as well as other techniques that can provide structural information on paramagnetic centers, such as EPR. 148 …”

Section: Metal Sites In Proteins and Other Biological Macromoleculesmentioning

confidence: 97%

Chapter 2. Solid‐State NMR in Drug Discovery and Development

Vogt¹

2013

New Applications of NMR in Drug Discovery and Development

View full text Add to dashboard Cite

Section: Metal Sites In Proteins and Other Biological Macromoleculesmentioning

confidence: 97%

Chapter 2. Solid‐State NMR in Drug Discovery and Development

Vogt¹

2013

New Applications of NMR in Drug Discovery and Development

View full text Add to dashboard Cite

“…EPR has been extensively used for assessing the nature of metal ions when present in certain distinct oxidation states, their enzymatic/functional activity (Brückner, 2010), and the role of some of these species in mediating tissue injury or some pathogenesis (Bartholomaus et al 2013;Fatfat et al 2014). Despite its strength, the application of EPR spectroscopy to study biomolecular metal centres in proteins and enzymes is an evolving field, albeit with insufficient attention (reviewed in Hagen, 2006Hagen, , 2009. EPR metallomics is concerned with the detection of paramagnetic metal centres including V IV , Mn II , Fe II , Fe III , Co II , Ni III , Ni I , Cu II , Mo V and W V .…”

Section: Measuring Rosmentioning

confidence: 99%

“…). Despite its strength, the application of EPR spectroscopy to study biomolecular metal centres in proteins and enzymes is an evolving field, albeit with insufficient attention (reviewed in Hagen, , ). EPR metallomics is concerned with the detection of paramagnetic metal centres including V IV , Mn II , Fe II , Fe III , Co II , Ni III , Ni I , Cu II , Mo V and W V .…”

Section: Introductionmentioning

confidence: 99%

Physiological and pathophysiological reactive oxygen species as probed by EPR spectroscopy: the underutilized research window on muscle ageing

Abdel‐Rahman

Mahmoud

Khalifa

et al. 2016

The Journal of Physiology

View full text Add to dashboard Cite

Reactive oxygen and nitrogen species (ROS and RNS) play crucial roles in triggering, mediating and regulating physiological and pathophysiological signal transduction pathways within the cell. Within the cell, ROS efflux is firmly controlled both spatially and temporally, making the study of ROS dynamics a challenging task. Different approaches have been developed for ROS assessment; however, many of these assays are not capable of direct identification or determination of subcellular localization of different ROS. Here we highlight electron paramagnetic resonance (EPR) spectroscopy as a powerful technique that is uniquely capable of addressing questions on ROS dynamics in different biological specimens and cellular compartments. Due to their critical importance in muscle functions and dysfunction, we discuss in some detail spin trapping of various ROS and focus on EPR detection of nitric oxide before highlighting how EPR can be utilized to probe biophysical characteristics of the environment surrounding a given stable radical. Despite the demonstrated ability of EPR spectroscopy to provide unique information on the identity, quantity, dynamics and environment of radical species, its applications in the field of muscle physiology, fatiguing and ageing are disproportionately infrequent. While reviewing the limited examples of successful EPR applications in muscle biology we conclude that the field would greatly benefit from more studies exploring ROS sources and kinetics by spin trapping, protein dynamics by site‐directed spin labelling, and membrane dynamics and global redox changes by spin probing EPR approaches.

show abstract

“…For more detailed information about EPR on transition metals and also organic radicals, the reader is referred to the corresponding textbooks. Good introductions into this field can be found for example in [14] and [32] or in comprehensive review articles like that by Hagen [31]. Comprehensive and detailed textbooks about EPR on transition metal ion and pulsed EPR are the books by Weil and Bolton [96], and Schweiger and Jeschke [77].…”

Section: Epr On Transition Metals and Organic Radicalsmentioning

confidence: 99%

Biomedical applications of electron paramagnetic resonance (EPR) spectroscopy

Klare

2012

Biomedical Spectroscopy and Imaging

View full text Add to dashboard Cite

EPR spectroscopy is the most straightforward-to-use technique for studying free radicals and other paramagnetic species and has therefore established itself in biomedical research and for clinical applications. EPR found its applications in oximetry, where this technique allows repeated measurements of oxygen in tissues -also in vivo and in combination with imaging techniques. The direct detection of paramagnetic species like transition metal ions by EPR allows elucidation of their specific roles in disease, for example how copper ions might be involved in the formation of amyloid-or amyloid-like fibrils in neurodegenerative diseases. For investigations on the structures of this protein aggregates, but also of other proteins or protein complexes with medical relevance the technique of site-directed spin labeling (SDSL) -the site-specific introduction of paramagnetic reporter groups into proteins or nucleic acids -is nowadays frequently used, especially in cases where other structural techniques are not applicable. Moreover, by direct detection of free radicals -also in combination with spin trapping methods, or application of SDSL, EPR spectroscopy can be also used for diagnostic purposes, e.g. in cancer diagnostics. This review gives an up-to-date overview about how EPR spectroscopy is currently used in biomedical research and clinical applications, with special emphasis on biomedical applications of SDSL EPR.

show abstract

EPR spectroscopy as a probe of metal centres in biological systems

Cited by 96 publications

References 69 publications

Chapter 2. Solid‐State NMR in Drug Discovery and Development

Chapter 2. Solid‐State NMR in Drug Discovery and Development

Physiological and pathophysiological reactive oxygen species as probed by EPR spectroscopy: the underutilized research window on muscle ageing

Biomedical applications of electron paramagnetic resonance (EPR) spectroscopy

Contact Info

Product

Resources

About