Epoxidation of propene by microbial cells immobilized on inorhanic supports

Abstract

Immobilization of gas-utilizing microorganism strains (Mycobacteria, Rhodococcus, methane-utilizers) on inorganic supports based on alumina, silicates, and carbon was carried out to develop heterogeneous biocatalysts for the biotechnologic processes, including the process of propene epoxidation. Adsorption ability of these microorganisms, biocatalytic properties of resting and immobilized bacterial cells, and effect of immobilization techniques on biocatalysis were studied. An approach of double immobilization using inorganic materials (supports and gel) was proposed as simple, universal, and available method to immobilize bacterial cells, resulting in a higher retention (up to 100%) of cells' enzymatic activity and enhanced stability.

Surface Properties of Mesoporous Carbon–Silica Gel Adsorbents

“…Additionally, they are potential polymer fillers, condensation agents for various dispersed media, and biocatalyst carriers (14,15). These materials are also used as intermediates in preparation of the carbon adsorbents and can provide desirable and original properties of finished adsorbents (16)(17)(18).…”

Methanol Biosynthesis by Methanotrophic Bacterial Cells: Effects of Various Immobilization Methods on Biocatalytic Activity of Immobilized Cellsa

“…A variety of supports were used on immobilized cells to produce propylene oxide. 7–11 Methods that involved the adsorption of whole cells on inorganic supports had a high commercial potential compared with the DEAE‐cellulose covalently linked immobilization 12 or entrapment. 11 The immobilized cells adsorbed on inorganic supports were suitable for use on a packed‐bed or fluidized‐bed bioreactor due to their good mechanical strength.…”

Epoxidation of Ethylene by Whole Cell Suspension of Methylosinus trichosporium IMV 3011

“…In recent years, epoxyethane synthesis has received considerable attention because of its possible industrial application [16]. The epoxidation of ethylene by methanotrophic bacteria in whole cell systems has been reported [8,[17][18][19]. MMO plays a critical role in ethylene epoxidation, which has been found in methanotrophic bacteria.…”