Epitope interactions of monoclonal antibodies targeting CD20 and their relationship to functional properties

Abstract

Epitope interactions of monoclonal antibodies targeting CD20 and their relationship to functional properties, mAbs, 5:1, 22-33,

Delineating the functional map of the interaction between nimotuzumab and the epidermal growth factor receptor

“…31,32 The identity of the epitopes can be deciphered through either structural 33 or functional methods. 34 The latter procedures are based on the identification of energetically critical residues contributing to epitope formation.…”

Lessons for the clinic from rituximab pharmacokinetics and pharmacodynamics

“…18 Indeed, the antibody may promote tumor antigen uptake and peptide presentation by dendritic cells, REVIEW REVIEW properties are not simply dependent upon the CD20 epitopes recognized by the antibodies. 25 RTX, like other unconjugated IgG1 antibodies, presents two types of binding: (1) Specific, through binding of Fab region of antibody to the CD20 target antigen ( Fig. 2A) and (2) Nonspecific, through binding of the antibody Fc region to Fcγ receptors (FcγR) on the immune effector cells, to the neonatal Fc receptor (FcRn) on different cell types and to C1q, the first complement component (Fig.…”

Obinutuzumab for relapsed or refractory indolent non-Hodgkin’s lymphomas

“…Obinutuzumab conformational structure is different to that of rituximab. First, obinutuzumab binds CD20 in a different overlapping epitope than rituximab, and in a different orientation [Klein et al 2013;Niederfellner et al 2011]. In comparison with rituximab, obinutuzumab is rotated 90° around the Fab middle axis and tilts 70° toward the carboxy terminus of the CD20 epitope.…”