Epithelial synthesis of tenascin at tips of growing bronchi and graded accumulation in basement membrane and mesenchyme

Koch, Manuel; Wehrle-Haller, Bernhard; Baumgärtner, Stefan; Spring, Jürg; Brubacher, Dorothee; Chiquet, Matthias

doi:10.1016/0014-4827(91)90368-5

Cited by 77 publications

(33 citation statements)

References 23 publications

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“…These findings are in concordance with the results of the present study and suggest that myofibroblasts are mainly tenascin-C-synthesizing cells in RDS and BPD. In early chicken lung bud, however, tenascin-C mRNA was found to be synthesized by the epithelium, inconsistent with the results of the present study (Koch et al 1991). The IHC expression for tenascin-C within alveolar and bronchiolar walls was stronger in patients with RDS and BPD than in controls.…”

Section: Discussioncontrasting

confidence: 99%

Tenascin-C Is Highly Expressed in Respiratory Distress Syndrome and Bronchopulmonary Dysplasia

Kaarteenaho‐Wiik

Kinnula

Soini

et al. 2002

J Histochem Cytochem.

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S U M M A R YTenascin-C is an extracellular matrix (ECM) glycoprotein expressed in human tissues during organogenesis and in fibrotic and neoplastic processes. We hypothesized that its expression would increase in human lung in neonatal disorders such as infant respiratory distress syndrome (RDS) and bronchopulmonary dysplasia (BPD). Tenascin-C expression was studied by immunohistochemistry (IHC) and mRNA in situ hybridization (ISH). The extent of tenascin-C immunoreactivity was scored as absent (0), low ( ϩ ), moderate ( ϩϩ ), strong ( ϩϩϩ ), or very strong ( ϩϩϩϩ ) separately in different types of pulmonary cells in controls (seven cases), RDS (19 cases), and BPD (12 cases). In controls, tenascin-C expression was low ( ϩ ) underneath alveolar and bronchiolar epithelium, moderate ( ϩϩ ) in intima of veins, and strong ( ϩϩϩ ) around chondrocytes. In RDS, tenascin-C expression was moderate ( ϩϩ ) or strong ( ϩϩϩ ) underneath both bronchiolar and often detached alveolar epithelium underlying hyaline membranes in the walls of dilated alveoli. In particular, the patients with RDS who survived for 1 day or more had strong expression of tenascin-C within alveolar walls. In patients with BPD, tenascin-C was very strongly ( ϩϩϩϩ ) expressed in the remodeled fibrotic alveolar walls underneath regenerative epithelium. Increased expression of tenascin-C mRNA was seen below the alveolar and bronchiolar epithelia in RDS and BPD. The cells in these locations showed ␣ -smooth muscle actin immunoreactivity, suggesting a myofibroblast phenotype. In conclusion, tenascin-C is highly expressed in the walls of alveoli and bronchioli in RDS and BPD, suggesting an association between the expression of this protein and the presence of these disorders.

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Section: Discussioncontrasting

confidence: 99%

Tenascin-C Is Highly Expressed in Respiratory Distress Syndrome and Bronchopulmonary Dysplasia

Kaarteenaho‐Wiik

Kinnula

Soini

et al. 2002

J Histochem Cytochem.

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“…This effect could correlate with tenascin functioning both as a differentiation factor (which could inhibit cell growth) and as a mitogen. A similar contrasting pattern of cellular responses has been observed in the effects of tenascin on attachment of neural cells or outgrowth of neuritcs (Wehrle-Haller et al, 1991). Of special interest is the observation that tenascin can enhance the mitogenic action of major polypeptide growth factors such as EGF, an effect which may be relevant to some of the above-mentioned processes.…”

Section: Discussionmentioning

confidence: 55%

“…Fibroncctin was prepared from conditioned medium of chick embryo fibroblasts (Wehrle-Haller et al, 1991). The protein was eluted from a gelatine-Sepharose column with 4 M urea, 20 mM Tris/HCl pH 7.4.…”

Section: Purification Of Tenascin and Other Proteinsmentioning

confidence: 99%

“…Studies focused on the involvement of tenascin in modulating cell growth have not been carried out previously. However, it has been shown that cells migrating on tcnascin Substrates or lining regions of tenascin expression (Gatchalian et al, 1989;Koch et al, 1991;Weis et al, 1991) were in a mitotic phase. As mentioned above, tenascin is re-expressed in malignant tumours and it has already been demonstrated that tenascin substrates can support growth of tumour cells (Chiquet-Ehrismann et al, 2986).…”

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confidence: 99%

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Tenascin: a modulator of cell growth

End

Panayotou

Entwistle

et al. 1992

European Journal of Biochemistry

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The large, multidomain extracellular matrix protein tenascin displays a markedly restricted tissue distribution during embryogenesis and remains present only in a few adult tissues. The protein is re‐expressed, however, during wound healing and in the stroma of malignant tumours. While a variety of studies have dealt with the important role of tenascin in the development of neural and non‐neural tissues, there is growing evidence that tenascin expression may be associated with proliferation of cells lining these tissues. The presence of repeating domains in tenascin similar to those in epidermal growth factor prompted us to investigate the ability of tenascin to modulate the growth of different cell types. Tenascin was actually found to be mitogenic for several cell types. This mitogenic activity, however, appears to be associated with a region in the fibronectin type III domains. The mitogenic mechanism is clearly distinct from pathways used by peptide growth factors such as epidermal growth factor and platelet‐derived growth factor, which activate the intrinsic tyrosine kinase activity of their cell‐surface receptors. However, we show that this large extracellular matrix molecule is efficiently internalised and may be processed by responding cells.

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“…It has been shown by in situ hybridization that in early lung development the epithelium at the tips of the growing bronchi is the source of the tenascin transcripts, but the protein is found quite distantly from the site of its synthesis (Koch et aL, 1991). Interestingly, Prieto et al (1990) have found that the embryonic lung epithelium contains tenascin mRNA, including the extra repeats, and thus should secrete the large tenascin variants.…”

Section: Discussionmentioning

confidence: 99%

Tenascin variants: differential binding to fibronectin and distinct distribution in cell cultures and tissues.

et al. 1991

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In the chicken, three tenascin variants have been characterized that are generated by alternative splicing of 3 of its 11 fibronectin type III repeats. Using monoclonal antibodies that react with common regions versus extra repeats of tenascin, we could distinguish and separate tenascin variants and investigate their interaction with fibronectin using multiple experimental procedures. Interestingly, in all assays used the smallest tenascin variant bound more strongly to fibronectin than the larger ones. These biochemical data were paralleled by the observation that in chick embryo fibroblast cultures only the smallest form of tenascin could be detected in the fibronectin-rich extracellular matrix network laid down by the cells. Furthermore, each tissue present in adult chicken gizzard contained a distinct set of tenascin variants. Those tissues particularly rich in extracellular matrix, such as the tendon, contained the smallest tenascin only. Intermediate-sized tenascin was present in smooth muscle, whereas the largest form was exclusively detectable underneath the epithelial lining of the villi. Thus it appears that cell type-specific forms of tenascin exist that are appropriate for the functional requirements of the respective extracellular matrices.

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Epithelial synthesis of tenascin at tips of growing bronchi and graded accumulation in basement membrane and mesenchyme

Cited by 77 publications

References 23 publications

Tenascin-C Is Highly Expressed in Respiratory Distress Syndrome and Bronchopulmonary Dysplasia

Tenascin-C Is Highly Expressed in Respiratory Distress Syndrome and Bronchopulmonary Dysplasia

Tenascin: a modulator of cell growth

Tenascin variants: differential binding to fibronectin and distinct distribution in cell cultures and tissues.

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