Epithelial Regulation of Innate Immunity to Respiratory Syncytial Virus

Garofalo, Roberto P.; Haeberle, Helene A.

doi:10.1165/ajrcmb.23.5.f204

Cited by 59 publications

(52 citation statements)

References 40 publications

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“…The sensitivity of the assay was 7.8 pg/ml. The specificity of the assay for traditionally classified cysteinyl leukotrienes (cys-LTs) LTC 4 and LTC 5 was 100%. The cross-reactivities …”

Section: Cysteinyl Leukotriene Determinationmentioning

confidence: 99%

Antioxidant Treatment Ameliorates Respiratory Syncytial Virus–induced Disease and Lung Inflammation

Castro

Guerrero-Plata

Suarez-Real

et al. 2006

Am J Respir Crit Care Med

Self Cite

145

154

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“…The sensitivity of the assay was 7.8 pg/ml. The specificity of the assay for traditionally classified cysteinyl leukotrienes (cys-LTs) LTC 4 and LTC 5 was 100%. The cross-reactivities …”

Section: Cysteinyl Leukotriene Determinationmentioning

confidence: 99%

Antioxidant Treatment Ameliorates Respiratory Syncytial Virus–induced Disease and Lung Inflammation

Castro

Guerrero-Plata

Suarez-Real

et al. 2006

Am J Respir Crit Care Med

Self Cite

145

154

View full text Add to dashboard Cite

“…Human A549 pulmonary type II epithelial cells (passages [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] were cultured in DMEM (4500 mg/l D-glucose), supplemented with 2 mM glutamine, 5% (v/v) inactivated FCS, streptomycin (100 lg/ml), and penicillin (100 IU/ml). The NHBE cells were cultured in complete BEGM medium (5 lg/ml insulin, 0Á5 lg/ml hydrocortisone, 10 lg/ml transferrin, 6Á5 ng/ml triiodothyronine, 0Á5 lg/ml epinephrine, 0Á5 ng/ml human epidermal growth factor, 0Á1 ng/ml retinoid acid, 50 lg/ml gentamicin, and 52 lg/ml bovine pituitary extract).…”

Section: Cell Culturementioning

confidence: 99%

Peroxisome proliferator‐activated receptor‐γ agonists inhibit respiratory syncytial virus‐induced expression of intercellular adhesion molecule‐1 in human lung epithelial cells

Arnold¹,

Neumann²,

König³

2007

Immunology

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SummaryRespiratory syncytial virus (RSV) is the major causative agent of severe lower respiratory tract disease and death in infants worldwide. The epithelial cells of the airways are the target cells for RSV infection and the site of the majority of the inflammation associated with the disease. However, despite five decades of intensive RSV research there exist neither an effective active vaccine nor a promising antiviral and anti-inflammatory therapy. Recently, peroxisome proliferator-activated receptor-c (PPAR-c), a member of the nuclear hormone receptor superfamily, has been shown to possess anti-inflammatory properties. Therefore, we hypothesized whether the detrimental increase of intercellular adhesion molecule-1 (ICAM-1) on RSV-infected lung epithelial cells (A549 and primary normal human bronchial epithelial cells (NHBE)) might be modulated by natural and synthetic PPAR-c agonists (15d-PGJ 2 , ciglitazone, troglitazone, Fmoc-Leu). Our data show that all PPAR-c agonists under study significantly downregulated the RSV-induced expression of ICAM-1 on A549-and NHBE cells in a dose-dependent manner resulting in a reduced b 2 integrinmediated adhesion of monocytic effector cells (U937) to RSV-infected A549 cell monolayers. In contrast, the PPAR-a agonist bezafibrate had no impact on the RSV-induced ICAM-1 expression. The reduced ICAM-1 expression was associated with a diminished ICAM-1 mRNA level and binding activity of nuclear factor-jB (p65/p50) in A549 cells. These findings suggest that PPARc agonists have beneficial effects in the suppression of the inflammatory response during RSV infection and therefore might have clinical efficacy in the course of severe RSV-infection.

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“…However, IL-8 is only one of a number of important inflammatory mediators released by respiratory epithelial cells in response to RSV infection. 33 It would be advantageous to apply studies of allele-specific expression more widely to screen for regulatory polymorphisms in other inflammatory mediators important to RSV pathogenesis.…”

Section: Upregulation Of Il-8 Disease-associated Haplotype D Hacking mentioning

confidence: 99%

Increased in vivo transcription of an IL-8 haplotype associated with respiratory syncytial virus disease-susceptibility

et al. 2004

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Epithelial Regulation of Innate Immunity to Respiratory Syncytial Virus

Cited by 59 publications

References 40 publications

Antioxidant Treatment Ameliorates Respiratory Syncytial Virus–induced Disease and Lung Inflammation

Antioxidant Treatment Ameliorates Respiratory Syncytial Virus–induced Disease and Lung Inflammation

Peroxisome proliferator‐activated receptor‐γ agonists inhibit respiratory syncytial virus‐induced expression of intercellular adhesion molecule‐1 in human lung epithelial cells

Increased in vivo transcription of an IL-8 haplotype associated with respiratory syncytial virus disease-susceptibility

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