Epithelial lining fluid free IGF-I-to-PAPP-A ratio is associated with bronchopulmonary dysplasia in preterm infants

Capoluongo, Ettore; Ameglio, Franco; Lulli, Paola; Minucci, Angelo; Santonocito, Concetta; Concolino, Paola; Stasio, Enrico Di; Boccacci, Simona; Vendettuoli, Valentina; Giuratrabocchetta, Giuseppe; Cunto, Angela De; Tana, Milena; Romagnoli, Costantino; Zuppi, Cecilia; Vento, Giovanni

doi:10.1152/ajpendo.00251.2006

Cited by 26 publications

(26 citation statements)

References 32 publications

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“…Proteins and urea were measured, as previously described [22,23]. Serum urea values obtained during the routine clinical tests in the same day of the lavage were used to calculate the serum-to-BALF urea ratio and obtain epithelial lining fluid (ELF) concentrations.…”

Section: Methodsmentioning

confidence: 99%

Clinical and biological role of secretory phospholipase A2 in acute respiratory distress syndrome infants

et al. 2013

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IntroductionSecretory phospholipase A2 is supposed to play a role in acute lung injury but no data are available for pediatric acute respiratory distress syndrome (ARDS). It is not clear which enzyme subtypes are secreted and what the relationships are between enzyme activity, biophysical and biochemical parameters, and clinical outcomes. We aimed to measure the enzyme and identify its subtypes and to study its biochemical and biophysical effect. The secondary aim was to correlate enzyme activity with clinical outcome.MethodsBronchoalveolar lavage was performed in 24 infants with ARDS and 14 controls with no lung disease. Samples were assayed for secretory phospholipase A2 and molecules related to its activity and expression. Western blotting and captive bubble surfactometry were also performed. Clinical data were real time downloaded.ResultsTumor necrosis factor-α (814 (506-2,499) vs. 287 (111-1,315) pg/mL; P = 0.04), enzyme activity (430 (253-600) vs. 149 (61-387) IU/mL; P = 0.01), free fatty acids (4.3 (2.8-8.6) vs. 2 (0.8-4.6) mM; P = 0.026), and minimum surface tension (25.6 ± 6.1 vs. 18 ± 1.8 mN/m; P = 0.006) were higher in ARDS than in controls. Phospholipids are lower in ARDS than in controls (76.5 (54-100) vs. 1,094 (536-2,907) μg/mL; P = 0.0001). Three enzyme subtypes were identified (-IIA, -V, -X), although in lower quantities in controls; another subtype (-IB) was mainly detected in ARDS. Significant correlations exist between enzyme activity, free fatty acids (ρ = 0.823; P < 0.001), and surface tension (ρ = 0.55; P < 0.028). Correlations also exist with intensive care stay (ρ = 0.54; P = 0.001), PRISM-III24 (ρ = 0.79; P< 0.001), duration of ventilation (ρ = 0.53; P = 0.002), and oxygen therapy (ρ = 0.54; P = 0.001).ConclusionsSecretory phospholipase A2 activity is raised in pediatric ARDS and constituted of four subtypes. Enzyme correlates with some inflammatory mediators, surface tension, and major clinical outcomes. Secretory phospholipase A2 may be a clinically relevant target in pediatric ARDS.

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Section: Methodsmentioning

confidence: 99%

Clinical and biological role of secretory phospholipase A2 in acute respiratory distress syndrome infants

et al. 2013

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“…Urea was measured using a high-sensitivity colorimetric method on an automatic analyser, as previously described [25]. All laboratory assays were performed in duplicate, by investigators blind to the clinical data.…”

Section: Additional Assaysmentioning

confidence: 99%

“…Data were corrected for the serum-to-BAL urea ratio in order to transform BAL into epithelial lining fluid (ELF) concentrations [25]. Measurement results in meconial solutions were compared as raw data or as per gram of meconium (after correction for the dilution).…”

Section: Statisticsmentioning

confidence: 99%

Role of distinct phospholipases A2 and their modulators in meconium aspiration syndrome in human neonates

et al. 2011

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“…The serum/BAL urea ratio was used to correct for dilution and obtain epithelial lining fluid (ELF) concentrations [12]. Surfactant function in BAL was studied via fluorescence analysis of adsorption kinetics into the air/liquid interface, as described previously [13].…”

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Effect of whole body hypothermia on inflammation and surfactant function in asphyxiated neonates

et al. 2014

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Hypothermia has become an evidence-based treatment for neonates with hypoxic-ischaemic encephalopathy (HIE) [1]. The usefulness of hypothermia is due to several mechanisms and among these the reduction in inflammation seems to play a relevant role [2,3]. When applied as whole body hypothermia (WBH), cooling may affect other organs as well. Recent data showed better respiratory outcomes and trends towards lower inflammation in WBH-treated preterm lambs [4], suggesting its possible usefulness to reduce lung injury through the modulation of the inflammatory pathway. By contrast, experiments with hibernating animals have shown that temperature induces significant adaptive changes to the surfactant composition and structure [5]. Nevertheless, no data are currently available in humans. Two case reports have recently described an infant [6] and an adult [7] with severe lung injury, whose ventilation had been facilitated by concurrent hypothermia. Since hypothermia is an accepted therapy only for HIE, we designed a preliminary translational study to investigate the effect of WBH on inflammation and surfactant status in neonates with HIE unaffected by any pulmonary injury.

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Epithelial lining fluid free IGF-I-to-PAPP-A ratio is associated with bronchopulmonary dysplasia in preterm infants

Cited by 26 publications

References 32 publications

Clinical and biological role of secretory phospholipase A2 in acute respiratory distress syndrome infants

Clinical and biological role of secretory phospholipase A2 in acute respiratory distress syndrome infants

Role of distinct phospholipases A2 and their modulators in meconium aspiration syndrome in human neonates

Effect of whole body hypothermia on inflammation and surfactant function in asphyxiated neonates

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