Epithelial heating rates with topical ciprofloxacin, ofloxacin, and ofloxacin with artificial tears after photorefractive keratectomy

Patel, Ghanshyam; Chuang, Alice Z.; Kiang, Elena; Ramesh, Narayan; Mitra, Shrabanee; Yee, Richard W.

doi:10.1016/s0886-3350(00)00411-9

Cited by 43 publications

(27 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The response of cellular proliferation and migration is related to the pH of topical eye drops [12,14], and the pH values of fluoroquinolones are different (Tarivid: pH 6.4, Cravit: pH 6.4, Ozex: pH 4.9-5.5, Gatiflo: pH 6.0 and Vigamox: pH 6.8). Patel et al [14] showed that ciprofloxacin is the least soluble of the commercially available fluoroquinolones due to its low pH, and they demonstrated that precipitation of the antibiotic in an epithelial defect might delay corneal re-epithelialization by blocking epithelial migration or inhibiting regeneration. Similarly, our study revealed that the relatively low pH fluoroquinolones such as tosufloxacin 0.3% induced delayed corneal epithelial migration as compared to that of ofloxacin 0.3% or levofloxacin 0.5% on the scratch assay.…”

Section: Discussionmentioning

confidence: 99%

Effects of Fluoroquinolone Eye Solutions without Preservatives on Human Corneal Epithelial Cells in vitro

et al. 2014

View full text Add to dashboard Cite

Aims: To evaluate the biologic effects of fluoroquinolone eye solutions without preservatives on cultured human corneal epithelial cells in vitro. Methods: We studied the effect of diverse generations of topical fluoroquinolones such as ofloxacin 0.3%, levofloxacin 0.5%, tosufloxacin 0.3%, moxifloxacin 0.5% and gatifloxacin 0.3% on cultured human corneal epithelial cells. MTT-based calorimetric assay, lactate dehydrogenase leakage (LDH) assay and scratch wound test were performed. Corneal epithelial cell morphologies were examined by performing inverted light microscopy and transmission electron microscopy. Results: In all topical fluoroquinolones, the metabolic activity of the corneal epithelial cells decreased in a time-dependent fashion and the LDH titer increased with longer exposure times. Especially, the LDH titers significantly increased after exposure to moxifloxacin 0.5% and gatifloxacin 0.3% compared with controls. The migration rates of the corneal epithelial cells were faster in ofloxacin 0.3% or levofloxacin 0.5% than other fluoroquinolones. Severe cellular morphological damage was observed after exposure to moxifloxacin 0.5% and gatifloxacin 0.3%. Conclusions: As moxifloxacin 0.5% and gatifloxacin 0.3% induced the toxic effect to the corneal epithelial cells, compared with other fluoroquinolones, the 4th fluoroquinolone eye solutions should be carefully used in case of the corneal epithelium is damaged by long duration of treatment or overdosage.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of Fluoroquinolone Eye Solutions without Preservatives on Human Corneal Epithelial Cells in vitro

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The release of cytokines, neuropeptides and chemokines involved in the wound-healing cascade contributes to a vicious cycle of inflammatory events and to the onset of corneal pain [2]. Twenty-four to 48 h after refractive surgery, most patients complain about painful corneal symptoms of various intensity due to the exposure of the damaged nerve endings [3]. …”

Section: Introductionmentioning

confidence: 99%

Effect of Basic Fibroblast Growth Factor in Transgenic Mice: Corneal Epithelial Healing Process after Excimer Laser Photoablation

Meduri

Scalinci²,

Morara³

et al. 2008

Ophthalmologica

View full text Add to dashboard Cite

Purpose: To evaluate the role of preparedbasic fibroblast growth factor (bFGF) eyedrops in corneal epithelial healing of transgenic mice after excimer laser photoablation. Materials and Methods: In our prospective case series, 60 eyes of 30 mice had uneventful bilateral excimer laser photoablation. In each mouse, 1 eye received the standard topical postoperative therapy with tobramicin, diclofenac and dexamethasone eyedrops, plusbFGF eyedrops 5 μg/10 μl PBS 3 times a day for 1 week, or until corneal reepithelialization was complete. The fellow eye served as the control and received the standard postoperative therapy plus placebo. The mice were monitored daily, commencing the day after surgery and for 7 days, in order to evaluate the corneal reepithelialization rate by using a video slitlamp camera with a cobalt blue light. The mean diameter of the corneal wounds was measured. Videotaped images were recorded and analyzed by computer planimetry. Results: All the eyes treated withbFGF eyedrops healed completely before day 5 after surgery, with a mean reepithelialization time of 90 ± 12 h (standard deviation); the mean reepithelialization time was 124 ± 10 h in those eyes receiving placebo. There were no statistically significant differences between the 2 groups in corneal haze presentation during the follow-up, perhaps because the time period was too brief (7 days). However, corneal clarity on slitlamp biomicroscopy was greater in the study group than in the control group. No side effects or toxic effects were documented. Conclusions: These data suggest that the bFGF significantly accelerates epithelial healing after excimer photoablation. A further clinical study should be performed to prove the results obtained in this study as well as the long-term efficacy of bFGF to prevent corneal haze.

show abstract

“…Immediately after laser photoablation, the release of cytokines, neuropeptides and chemokines involved in the wound-healing cascade contribute to a vicious cycle of inflammatory events and to the onset of corneal pain [2]. Subsequently, 24–48 h following refractive surgery, most patients complain of painful corneal symptoms of varying intensity because the damaged nerve endings are exposed [3]. …”

Section: Introductionmentioning

confidence: 99%

Effect of Cysteine in Transgenic Mice on Healing of Corneal Epithelium after Excimer Laser Photoablation

Scorolli

Meduri²,

Morara³

et al. 2008

Ophthalmologica

View full text Add to dashboard Cite

Purpose: To evaluate the role of prepared cysteine per os in corneal epithelial healing in transgenic mice (B6(A)-Rpe65rd12/J) after excimer laser photoablation. Materials and Methods: In our prospective case series, 60 eyes of 30 mice had uneventful bilateral excimer laser photoablation. A first group, composed of 15 mice, received standard topical post-operative therapy with tobramycin, diclofenac and dexamethasone eyedrops, pluscysteine 5 mg 5 μg/10 μl phosphate-buffered saline (3 times a day for 1 week) per os or until corneal re-epithelialization was complete. The control group of 15 mice received standard post-operative therapy plus placebos. The mice were monitored daily, commencing the day after surgery, for 7 days to evaluate the corneal re-epithelialization rate using a video slit-lamp camera with a cobalt blue light. The mean diameters of corneal wounds were measured. Videotaped images were recorded and analysed by computer planimetry. Results: All the eyes of the mice treated withcysteine healed completely by day 5 after surgery, with a mean re-epithelialization time of 92 ± 10 h (SD); the mean re-epithelialization time was 125 ± 8 h in eyes that received the placebo. There were no statistically significant differences between the 2 groups in corneal haze presentation during follow-up, perhaps because the time period was too brief (7 days). However, corneal clarity in the study group was greater than in the control group, as shown on slit-lamp biomicroscopy. No side effects or toxic effects were documented. Conclusions: These data suggest that cysteine significantly accelerates epithelial healing after excimer photoablation. A further clinical study should be performed to confirm the results obtained in this study, and the long-term efficacy of cysteine in preventing corneal haze.

show abstract

Epithelial heating rates with topical ciprofloxacin, ofloxacin, and ofloxacin with artificial tears after photorefractive keratectomy

Abstract: Ofloxacin with Refresh Plus and ofloxacin alone had a more positive effect on epithelial healing than ciprofloxacin. The ciprofloxacin eyes were significantly more prone to impaired or delayed wound healing and to the development of corneal haze.

Cited by 43 publications

References 13 publications

Effects of Fluoroquinolone Eye Solutions without Preservatives on Human Corneal Epithelial Cells in vitro

Effects of Fluoroquinolone Eye Solutions without Preservatives on Human Corneal Epithelial Cells in vitro

Effect of Basic Fibroblast Growth Factor in Transgenic Mice: Corneal Epithelial Healing Process after Excimer Laser Photoablation

Effect of Cysteine in Transgenic Mice on Healing of Corneal Epithelium after Excimer Laser Photoablation

Contact Info

Product

Resources

About